Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells

Fahimipour, Farahnaz; Dashtimoghadam, Erfan; Rasoulianboroujeni, Morteza; Yazdimamaghani, Mostafa; Khoshroo, Kimia; Tahriri, Mohammadreza; Yadegari, Amir; González, José A.; Vashaee, Daryoosh; Lobner, Doug; Kashi, Tahereh Sadat Jafarzadeh; Tayebi, Lobat

doi:10.1016/j.dental.2017.10.001

Cited by 28 publications

(20 citation statements)

References 48 publications

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“…All surgical procedures were implemented according to an experimental protocol approved by the Institutional Animal Care and Use Committee (IACUC) of Marquette University 20 . All experiments were performed in accordance with relevant guidelines and regulations.…”

Section: Methodsmentioning

confidence: 99%

“…However, the long-term survival and functional state of the cells to actively proliferate and differentiate still require further adjustments. Manipulation of the extracellular matrix (ECM) microenvironment to mimic the cell niche is a promising approach to enhance stem cell based clinical regenerative outcomes 19 , 20 . The niche highly dynamic key components comprise direct interactions between stem cells and secreted signaling molecules—ECM physical and chemical cues, along with environmental changes such as hypoxia 19 – 21 .…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

Dashtimoghadam

Fahimipour

Tongas

et al. 2020

Sci Rep

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Wound instability and poor functional vascularization in bone tissue engineering lead to lack of tissue integration and ultimate failure of engineered grafts. In order to harness the regenerative potential of growth factors and stimulate bone healing, present study aims to design multifunctional cell therapy microcarriers with the capability of sequential delivery of essential growth factors, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF). An on-chip double emulsion method was implemented to generate monodisperse VEGF encapsulated microcarriers. Bio-inspired poly(3,4-dihydroxyphenethylamine) (PDA) was then functionalized to the microcarriers surface for BMP-2 conjugation. The microcarriers were seeded with mesenchymal stem cells (MSCs) using a dynamic culture technique for cells expansion. Finally, the microcarriers were incorporated into an injectable alginate-RGD hydrogel laden with endothelial cells (ECs) for further analysis. The DNA and calcium content, as well as ALP activity of the construct were analyzed. The confocal fluorescent microscopy was employed to monitor the MSCs and tunneling structure of ECs. Eventually, the capability of developed microcarriers for bone tissue formation was examined in vivo. Microfluidic platform generated monodisperse VEGF-loaded PLGA microcarriers with size-dependent release patterns. Microcarriers generated with the on-chip technique showed more sustained VEGF release profiles compared to the conventional bulk mixing method. The PDA functionalization of microcarriers surface not only provided immobilization of BMP-2 with prolonged bioavailability, but also enhanced the attachment and proliferation of MSCs. Dynamic culturing of microcarriers showcased their great potential to boost MSCs population required for stem cell therapy of bone defects. ALP activity and calcium content analysis of MSCs-laden microcarriers loaded into injectable hydrogels revealed their capability of tunneling formation, vascular cell growth and osteogenic differentiation. The in vivo histology and real-time polymerase chain reaction analysis revealed that transplantation of MSCladen microcarriers supports ectopic bone formation in the rat model. The presented approach to design bioactive microcarriers offer sustained sequential delivery of bone ECM chemical cues and offer an ideal stabilized 3D microenvironment for patient-specific cell therapy applications. The proposed methodology is readily expandable to integrate other cells and cytokines in a tuned spatiotemporal manner for personalized regenerative medicine. Bone healing is a multifaceted developmental process which involves multiple signaling molecules in a specific localization and orchestration to induce vascularization and osteogenesis 1-3. Vascularization plays a leading role in tissue repair and regeneration 4,5. However, conventional constructs for bone tissue engineering are poorly vascularized, which typically leads to their weak integration and survival 6. On the other hand, slow penetration of the...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

Dashtimoghadam

Fahimipour

Tongas

et al. 2020

Sci Rep

Self Cite

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“…[63,64] Rather, some printing materials could be advantageously formed owing to the inordinate flowability and reduction of shear viscosity with increasing printing temperature. [65] Type 2 surfaces possessed proper widths and heights in terms of uniformity with; however, some fatal defects presented in specimens printed between 190 and 200 C. The specimen printed at 210 C had no critical defects, but its uniformity and flatness were relatively poor ( Figure S4). Thus, in the type 2 printing temperature range, 220 C was the most appropriate to ensure the best surface quality of the 3D-printed PLA structure in terms of uniformity.…”

Section: Printing-temperature-dependent Defective Surface and Mechamentioning

confidence: 99%

3D‐printed polymer packing structures: Uniformity of morphology and mechanical properties via microprocessing conditions

Kim

Korolovych

Muhlbauer

et al. 2020

J of Applied Polymer Sci

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Three‐dimensional (3D) printing is an attractive approach to fabricate highly porous extremely lightweight structures for architecture antivibrational packaging. We report 3D printing processing of model packaging structures using biodegradable poly(lactic acid) (PLA) as a source material, with acrylonitrile butadiene styrene (ABS) utilized as a common 3D printing source material as a traditional benchmarked material. The effects of printing temperature, speed, and layer morphology on the layer‐by‐layer 3D‐printed structures and their mechanical properties were considered. Three different characteristic morphologies were identified based on printing temperature; the microscopic surface roughness was dependent on the printing speed and layer height. We demonstrate that the mechanical performances and surface properties of 3D‐printed PLA structures could be improved by optimization of printing conditions. Specifically, we evaluate that these PLA‐based 3D structures printed exhibited better surface qualities and enhanced mechanical performance than traditional ABS‐based structures. Results showed that the PLA‐based 3D structures possessed the favorable mechanical performance with 34% higher Young's modulus and 23% higher tensile strength in comparison to the ABS‐based 3D structures. This study provides guidelines for achieving high‐quality 3D‐printed lightweight structures, including smooth surfaces and durable mechanical properties, and serves as a framework to create biodegradable 3D‐printed parts for human use.

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“…Also, bone defects resulting from accidents, infection, and tumors require large amount of bone grafts, which are relatively in shortage in clinical practice. 2,3 As a result, bone tissue engineering is developing as an alternative approach to solve these problems. 4 Electrospinning has emerged as a promising method to fabricate scaffolds in tissue engineering in recent decades.…”

Section: Introductionmentioning

confidence: 99%

Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells

Chen

Zhou

et al. 2018

IJN

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IntroductionScaffold structure plays a vital role in cell behaviors. Compared with two-dimensional structure, 3D scaffolds can mimic natural extracellular matrix (ECM) and promote cell–cell and cell–matrix interactions. The combination of osteoconductive scaffolds and osteoinductive growth factors is considered to have synergistic effects on bone regeneration.Materials and methodsIn this study, core–shell poly(lactide-co-glycolide) (PLGA)/polycaprolactone (PCL)–BMP-2 (PP–B) fibrous scaffolds were prepared through coaxial electrospinning. Next, we fabricated 3D scaffolds based on PP–B fibers with thermally induced self-agglomeration (TISA) method and compared with conventional PLGA/PCL scaffolds in terms of scaffold morphology and BMP-2 release behaviors. Then, rat adipose-derived stem cells (rADSCs) were seeded on the scaffolds, and the effects on cell proliferation, cell morphology, and osteogenic differentiation of rADSCs were detected.ResultsThe results demonstrated that 3D scaffold incorporated with BMP-2 significantly increased proliferation and osteogenic differentiation of rADSCs, followed by PP–B group.ConclusionOur findings indicate that scaffolds with 3D structure and osteoinductive growth factors have great potential in bone tissue engineering.

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Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells

Abstract: The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.

Cited by 28 publications

References 48 publications

Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

3D‐printed polymer packing structures: Uniformity of morphology and mechanical properties via microprocessing conditions

Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells

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Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells

Abstract: The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.

Cited by 28 publications

References 48 publications

Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

Microfluidic fabrication of microcarriers with sequential delivery of VEGF and BMP-2 for bone regeneration

3D‐printed polymer packing structures: Uniformity of morphology and mechanical properties via microprocessing conditions

Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core&ndash;shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells

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Thermally induced self-agglomeration 3D scaffolds with BMP-2-loaded core–shell fibers for enhanced osteogenic differentiation of rat adipose-derived stem cells