Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Tada, Yasuhiro; Takezawa, Toshiaki; Tani, Akiko; Nakamura, Tatsuo; Omori, Koichi

doi:10.3109/00016489.2012.654851

Cited by 15 publications

(14 citation statements)

References 19 publications

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“…Collagen vitrigel membrane is a transparent biomaterial characterized by a highly organized fibrillar nanostructure (Takezawa, Nitani, Shimo‐Oka, & Takayama, ; Takezawa, Ozaki, Nitani, Takabayashi, & Shimo‐Oka, ). Tracheal reconstruction studies have indicated the feasibility of using collagen vitrigel membrane for accelerating epithelial regeneration (Tada et al, ; Tada, Takezawa, Tani, Nakamura, & Omori, ). Therefore, collagen vitrigel membrane is considered as a candidate scaffold for organizing iPSC‐derived airway epithelial sheet graft.…”

Section: Introductionmentioning

confidence: 99%

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Okuyama

Ohnishi

Nakamura

et al. 2019

J Tissue Eng Regen Med

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Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There is no consensus on the best approach for the restoration of tracheal functionality in patients with tracheal defects. Artificial grafts made of polypropylene and collagen sponge have been clinically used by our group. However, 2 months are required to achieve adequate epithelialization of the grafts in humans. This study aimed to investigate the feasibility of transplantation therapy using an artificial trachea with human‐induced pluripotent stem cell (hiPSC)‐derived multiciliated airway cells (hiPSC‐MCACs). Collagen vitrigel membrane, a biocompatible and absorbable material, was used as a scaffold to cover the artificial trachea with hiPSC‐MCACs. Analyses of hiPSC‐MCACs on collagen vitrigel membrane were performed by immunocytochemistry and electron microscopy and by assessing ciliary beat frequency. Along with the artificial trachea, hiPSC‐MCACs were transplanted into surgically created tracheal defects of immunodeficient rats. The survival of transplanted cells was histologically evaluated at 1 and 2 weeks after the transplantation. The hiPSC‐MCACs exhibited motile cilia on collagen vitrigel membrane. The surviving hiPSC‐MCACs were observed in the endotracheal epithelium of the tracheal defect at 1 and 2 weeks after transplantation. These results suggest that hiPSC‐MCAC is a useful candidate for tracheal reconstruction.

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Section: Introductionmentioning

confidence: 99%

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Okuyama

Ohnishi

Nakamura

et al. 2019

J Tissue Eng Regen Med

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“…To promote regeneration of the tracheal epithelium without a cell source, gel scaffold or growth factor was impregnated into gelatin hydrogel for tracheal defect repair [14,28,29]. Recently, collagen has been used for osteochondral regeneration [30][31][32], and in the present study, PC-NF with or without hUCS showed good cartilage regeneration.…”

Section: Discussionmentioning

confidence: 78%

Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection

Jang¹,

Jang

Cho

et al. 2014

International Journal of Pediatric Otorhinolaryngology

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“…The possible reasons for the different findings include differences in the environment between in vivo and in vitro experimentation or differences in the defect position and size. Collagen vitrigel is a carrier with good tissue‐engraftment capacity and whose implantation has been reported to induce tissue repair . This implies that, compared with other carriers, the sustained, slow release of TGF‐β into articular cartilage produces more effective results.…”

Section: Discussionmentioning

confidence: 99%

“…Various studies of tissue regeneration have made use of the advantages of collagen vitrigel membrane . Collagen xerogel membranes, defined as dried collagen vitrigel membranes without free water, can be prepared by simply revitrifying collagen vitrigel membranes on a separable sheet.…”

Section: Methodsmentioning

confidence: 99%

Effects of a cell‐free method using collagen vitrigel incorporating TGF‐β1 on articular cartilage repair in a rabbit osteochondral defect model

et al. 2016

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We studied the ability of collagen vitrigel material to repair cartilage in vivo when used alone or with transforming growth factor-β (TGF-β). We measured the time course and quantity of TGF-β1 released from the collagen vitrigel in vitro to quantify the controlled release of TGF-β1. Over 14 days, 0.91 ng of TGF-β was released from the collagen vitrigel. Osteochondral defects were made in the femoral trochlear groove in 36 Japanese white rabbits, which were divided into three groups: untreated group (group A), collagen vitrigel-implanted group (group B), and TGF-β1-incorporated collagen vitrigel-implanted group (group C). The weight distribution ratio between the affected and unaffected limbs served as an indicator of pain. Animals were sacrificed at 4 and 12 weeks after surgery, and their tissues were assessed histologically. The weight distribution ratio increased in all groups and did not differ significantly between groups at 12 weeks. Group A needed 6 weeks to attain maximum improvement, and groups B and C showed near-maximum improvement at 4 and 2 weeks, respectively. The International Cartilage Repair Society II score improved significantly in group C relative to the other groups. These findings suggest that sustained, slow release of TGF-β caused early pain mitigation and cartilage repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2592-2602, 2017.

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Collagen vitrigel scaffold for regenerative medicine of the trachea: Experimental study and quantitative evaluation

Cited by 15 publications

References 19 publications

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Tracheal regeneration using polycaprolactone/collagen-nanofiber coated with umbilical cord serum after partial resection

Effects of a cell‐free method using collagen vitrigel incorporating TGF‐β1 on articular cartilage repair in a rabbit osteochondral defect model

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