Collagen Type I mRNA Levels in Cultured Human Lamina Cribrosa Cells: Effects of Elevated Hydrostatic Pressure

Yang, Jian; Neufeld, Arthur H.; Zorn, M. B.; Hernandez, M. Rosario

doi:10.1006/exer.1993.1070

Cited by 42 publications

(27 citation statements)

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“…As we will demonstrate in subsequent sections, there is overlap between the pressure-induced phenotype and glaucomatous cellular profiles that have been described previously (Hernandez et al, 2002;Nickells, 2007). Similarly, we found that extended HP treatment of ONH astrocytes (72-120 hr) induces further changes in cellular phenotype with respect to the expression of genes that affect cell motility (Miao et al, 2010) and cell morphology (Yang et al, 1993). …”

Section: Microarray Data Analysissupporting

confidence: 84%

“…Cluster 3 genes are found in pathways leading to cytoskeleton remodeling, transport, and other signaling processes. ONH astrocytes undergo changes in morphology after being subjected to elevated HP (Yang et al, 1993) and up-regulation of cytoskeletal signaling processes is consistent with these properties. Moreover, altered cytoskeletal , transport, and cell adhesion gene expression is also found in populations of glaucomatous astrocytes .…”

Section: Hp-induced Cellular Processes In Onh Astrocytessupporting

confidence: 60%

“…Thus, our in vitro IOP model employs elevated hydrostatic pressure in an environmental chamber (Salvador-Silva et al, 2001) that compensates for gas composition and changes in media over time Ricard et al, 2000). This model system has been used in a number of studies to study changes in bimolecular and cellular properties associated with this induced stress Salvador-Silva et al, 2004;Salvador-Silva et al, 2001;Hernandez et al, 2000;Malone et al, 2007;Yang et al, 1993;Chen et al, 2009;Miao et al, 2010).…”

Section: Model Systems For Glaucomatous Astrocytesmentioning

confidence: 99%

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Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Lukas¹,

Du²,

Miao³

et al. 2011

The Mystery of Glaucoma

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Section: Microarray Data Analysissupporting

confidence: 84%

Section: Hp-induced Cellular Processes In Onh Astrocytessupporting

confidence: 60%

Section: Model Systems For Glaucomatous Astrocytesmentioning

confidence: 99%

See 1 more Smart Citation

Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Lukas¹,

Du²,

Miao³

et al. 2011

The Mystery of Glaucoma

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“…192,196,197 The increased interstitial hydrostatic pressure can, in turn, influence the function of the resident cells and the composition of ECM itself. In vitro studies have shown that exposure to elevated hydrostatic pressure increased production of collagen type I and aggrecan, 198 decreased MMP2 and MMP3 expression, 199,200 decreased collagen I, and increased sulfated glycosaminoglycans production. 201 These changes further alter ECM composition influencing intercellular transportation.…”

Section: Matrix As a Hydrostatic Environmentmentioning

confidence: 99%

Matrix as an Interstitial Transport System

Fan

Creemers²,

Kassiri³

2014

Circulation Research

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T he extracellular space, or the interstitium, can be broadly divided into a fluid phase (water, electrolytes, nutrients, and some plasma proteins) and the extracellular matrix (ECM). In the heart, the ECM comprises structural proteins (primarily collagen types I and III) that form the fibrillar structure of the matrix; the basement membrane that forms an interface between the cardiomyocytes and the interstitial space as well as the molecules that mediate the cell-ECM connection; and the nonstructural proteins such as proteoglycans. Molecules traveling in the extracellular space come in contact with different ECM components, and these interactions determine the direction, speed, and distance of their transport, whereas the ECM can also provide a reservoir for several growth factors and cytokines. Moreover, the cell-ECM connection allows for transmission of signals across the cell membrane. Therefore, in addition to the classical role of ECM in providing structural support, it contributes to a multitude of intra-and extracellular events. Several thorough reviews have been published recently on the structural properties of the ECM and its remodeling in heart disease. [1][2][3] In this review, we will discuss less explored aspects of ECM function, such as its conductivity in extracellular transportation, its role in cell-cell interactions, and different modes of cell-cell communication through the extracellular space.

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“…Concerning the study of glaucoma, EHP has been used to mimic ocular hypertension. Several studies have reported the effect of EHP on cells of the trabecular meshwork [18][19][20] and Schlemm's canal [21] and other cell types [22] . The mechanisms whereby retinal cells sense mechanical pressure have not yet been completely elucidated, yet some possibilities have been considered [for a review, see 23 ].…”

Section: Advantages Of Using In Vitro Models To Study Glaucomamentioning

confidence: 99%

Modeling Human Glaucoma: Lessons from the in vitro Models

Aires

Ambrósio²,

Santiago³

2016

Ophthalmic Res

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Glaucoma, a leading cause of blindness worldwide, is a degenerative disease characterized by retinal ganglion cell (RGC) loss and optic nerve atrophy. Elevated intraocular pressure (IOP) is a main risk factor for onset and progression of the disease. Since increased IOP is the only modifiable risk factor, relevant models for glaucoma would comprise RGC and optic nerve damage triggered by ocular hypertension. Animal models of glaucoma have greatly contributed to the understanding of the molecular mechanisms of this pathology, and they have also facilitated the development of new pharmacological interventions. Although animal models of glaucoma have provided valuable information about the disease, there is still no ideal model for studying glaucoma due to its complexity. There is a recognized demand for in vitro models that can replace or reduce the need for animal experiments. Several in vitro models have emerged as a great opportunity in the field of glaucoma research, helping to clarify the mechanisms involved in disease progression. Several types of equipment have been developed to expose cells and tissue cultures to elevated pressures. Herein, we discuss the methodology used to increase pressure, the main findings, and the relevance of in vitro models for the study of the pathophysiology of glaucoma.

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Collagen Type I mRNA Levels in Cultured Human Lamina Cribrosa Cells: Effects of Elevated Hydrostatic Pressure

Cited by 42 publications

References 0 publications

Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Matrix as an Interstitial Transport System

Modeling Human Glaucoma: Lessons from the in vitro Models

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