Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Lukas, Thomas J.; Du, Pan; Miao, Haixi; Lin, Simon

doi:10.5772/18700

Cited by 1 publication

(1 citation statement)

References 71 publications

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“…In contrast to the genes in clusters A and B, the genes in cluster C and D were all downregulated in CAβ3 cells relative to WTβ3 cells. Of the three genes found in cluster C, only ARHGAP24 (cluster C, FC = −2.04), which encodes a protein that participates in cell-ECM adhesion complexes in cultured HTM cells [88], has any relevance to TM function or glaucoma as it has also been identified in cultured ONH astrocytes as being phosphorylated in response to increases in hydrostatic pressure [89]. LPXN (FC = −1.51; cluster D) encodes a focal adhesion protein that can also function as a transcription factor [90].…”

Section: Hierarchical Clustering Reveals Differences In Genes Encoding Gtp-binding and Cytoskeleton-associated Proteinsmentioning

confidence: 99%

Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

Filla

Meyer

Faralli

et al. 2021

Cells

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Studies from our laboratory have suggested that activation of αvβ3 integrin-mediated signaling could contribute to the fibrotic-like changes observed in primary open angle glaucoma (POAG) and glucocorticoid-induced glaucoma. To determine how αvβ3 integrin signaling could be involved in this process, RNA-Seq analysis was used to analyze the transcriptomes of immortalized trabecular meshwork (TM) cell lines overexpressing either a control vector or a wild type (WT) or a constitutively active (CA) αvβ3 integrin. Compared to control cells, hierarchical clustering, PANTHER pathway and protein-protein interaction (PPI) analysis of cells overexpressing WT-αvβ3 integrin or CA-αvβ3 integrin resulted in a significant differential expression of genes encoding for transcription factors, adhesion and cytoskeleton proteins, extracellular matrix (ECM) proteins, cytokines and GTPases. Cells overexpressing a CA-αvβ3 integrin also demonstrated an enrichment for genes encoding proteins found in TGFβ2, Wnt and cadherin signaling pathways all of which have been implicated in POAG pathogenesis. These changes were not observed in cells overexpressing WT-αvβ3 integrin. Our results suggest that activation of αvβ3 integrin signaling in TM cells could have significant impacts on TM function and POAG pathogenesis.

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Section: Hierarchical Clustering Reveals Differences In Genes Encoding Gtp-binding and Cytoskeleton-associated Proteinsmentioning

confidence: 99%

Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

Filla

Meyer

Faralli

et al. 2021

Cells

View full text Add to dashboard Cite

show abstract

Differential Effects of Elevated Hydrostatic Pressure on Gene Expression and Protein Phosphorylation in Optic Nerve Head Astrocytes

Cited by 1 publication

References 71 publications

Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

Overexpression and Activation of αvβ3 Integrin Differentially Affects TGFβ2 Signaling in Human Trabecular Meshwork Cells

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