Collagen synthesis and collagenase activity of cryopreserved heart valves

Abstract

Although the collagen synthesis of cryopreserved valves was relatively maintained, the protein synthesis was highly diminished, and the collagenolysis ability was activated immediately after the thawing process. These results imply that the cryopreservation procedure itself may cause the collagen metabolism to be on the degradable side, which will lead to valve failure.

Potential of Human Bone Marrow Stromal Cells to Accelerate Wound Healing in Vitro

“…Although there has recently been much interest in the use of topical growth factors for the treatment of chronic wounds such as diabetic foot ulcers, the effects have not generally been very dramatic. [1][2][3][4] In other attempts to deliver growth factors to wounds, fibroblast implants, which are able to adjust to a wound's environment and provide the desired growth factors and other substances that maybe be lacking in a chronic wound, have been developed as biologic wound dressings. [5][6][7][8] Bone marrow stroma is the source of mesenchymal stem cells that may serve as long-lasting precursors for bone, cartilage, muscle, and connective tissues.…”

Functional restoration of endothelial cells of the cryopreserved heart valve

“…Details of regenerated cells of a cryopreserved heart valve were not verifi ed, but endothelial cells of porcine aortic cusps are likely to sustain more serious damage from the current cryopreservation techniques and management than do fi broblasts. 4,5 Although ex vivo static environments with several growth factor-rich culture solutions are able to restore the cryopreserved heart valve according to the level of the damage, endothelial cells seem to have less regenerative capacity than other cells in cryopreserved heart valves.…”

“…[1][2][3] Our previous studies demonstrated that cryopreserved heart valves before implantation displayed inevitable cell injury, collagenolytic activation, and latent degradation of collagen synthesis, which would lead to valve deterioration. 4,5 Other investigators also revealed that cryopreserved heart valves rapidly lost their cellular components and normal tissue architecture after implantation. 6,7 Current understanding relevant to the procurement of optimal allograft heart valves is that an allograft with less-viable cellular elements and wellpreserved matrices may be ideal for provoking the least donor-specifi c immune response.…”

Functional role of scaffold geometries as a template for physiological ECM formation: evaluation of collagen 3D assembly

“…Scanning electron microscopy (SEM) imaging further confirmed images obtained through CLM analysis (Jones et al ., ). Collagen production and organization were analysed using CLM after direct red 80 staining (Dolber and Spach, ; Kano et al ., ; Sweat et al ., ; Taatjes et al ., ).…”