Potential of Human Bone Marrow Stromal Cells to Accelerate Wound Healing in Vitro

Han, Seung Kyu; Yoon, Taekyung; Lee, Dong Geun; Lee, Min Ah; Kim, Woo Kyung

doi:10.1097/01.sap.0000178809.01289.10

Cited by 72 publications

(58 citation statements)

References 36 publications

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“…In addition to the acceleration of wound closure, the MSCs treatment strongly enhances the scar quality, which was associated to a greater quantity of collagen within the healed tissue increasing the tensile strength [13]. These cells' synthesis of larger amounts of collagen and growth factors, than native dermal fibroblasts, proves their therapeutic efficiency in cutaneous repair [14]. Together, MSC differentiation helps to regenerate damaged tissue, while their paracrine signaling accelerates reepithelialization and fibroproliferation during wound repair [15].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

Linard

Tissedre

Busson

et al. 2015

Stem Cells and Development

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Mesenchymal stem cell (MSC) therapy has recently been investigated as a potential treatment for cutaneous radiation burns. We tested the hypothesis that injection of local gingival fibroblasts (GFs) would promote healing of radiation burn lesions and compared results with those for MSC transplantation. Human clinicalgrade GFs or bone marrow-derived MSCs were intradermally injected into mice 21 days after local leg irradiation. Immunostaining and real-time PCR analysis were used to assess the effects of each treatment on extracellular matrix remodeling and inflammation in skin on days 28 and 50 postirradiation. GFs induced the early development of thick, fully regenerated epidermis, skin appendages, and hair follicles, earlier than MSCs did. The acceleration of wound healing by GFs involved rearrangement of the deposited collagen, modification of the Col/MMP/TIMP balance, and modulation of the expression and localization of tenascin-C and of the expression of growth factors (VEGF, EGF, and FGF7). As MSC treatment did, GF injection decreased the irradiation-induced inflammatory response and switched the differentiation of macrophages toward an M2-like phenotype, characterized by CD163 + macrophage infiltration and strong expression of arginase-1. These findings indicate that GFs are an attractive target for regenerative medicine, for easier to collect, can grow in culture, and promote cutaneous wound healing in irradiation burn lesions.

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Section: Introductionmentioning

confidence: 99%

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

Linard

Tissedre

Busson

et al. 2015

Stem Cells and Development

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show abstract

“…In in vitro studies, MSCs have demonstrated a number of properties, which can facilitate tissue regeneration and even accelerate this process, including synthesis of some growth factors, cytokines, collagen and matrix metalloproteinases, angiogenic factors [26,28,29]. Besides, they are capable of providing migration of other skin cells such as keratinocytes [30].…”

Section: A Cellular Componentmentioning

confidence: 99%

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

Meleshina¹,

Bystrova²,

Rogovaya³

et al. 2017

Sovrem Tehnol Med

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Development and introduction of new biotechnological analogues (equivalents) of tissues and organs into clinical practice, such as human skin equivalents (SE), designed for temporal or permanent replacement of damaged or destroyed tissue, remains an urgent problem of regenerative medicine. Currently, full-thickness SE as well as separate skin layers, which include living cells of different types, are being created and investigated. Since the ideal skin substitutes have not been created, the efforts of researchers in many countries are aimed at solving this problem.In our review, we present a comparative analysis of existing SE, both commercial and those being at the stage of preclinical study, analyze their structure and feasibility of application for solving experimental and clinical tasks. Characteristics of the three main variants of SE have also been considered. Examples of stem cell application for creation of skin equivalents have been given. The main advantages of using stem cells as a cell component of skin equivalents have been described.

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“…Recently bone marrow, peripheral blood and umbilical cord blood have all been used as topical therapies to modulate the healing response in dermal wounds and change the nature of chronic wounds to acute wounds [29][30][31]. It is possible that fibrocytes and KLC, which are stem cells derivatives, are involved.…”

Section: Application In Plastic Surgerymentioning

confidence: 99%

Fibrocyte and Keratinocyte-Like Cells and Their Role in Burn Wound Healing

Curran¹,

Ghahary²

2012

Surgery

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Burn injuries affect millions of people every year and dermal fibrosis is a common complication for the victims. This disfigurement has functional and cosmetic consequences and many research groups have made it the pivot of their work to understand the mechanisms that underlie its development. Although a significant amount of progress has been made in wound healing processes, the complexity of events involved makes it very difficult to come up with a single strategy to prevent this devastating fibrotic condition. Inflammation is considered one pre-disposing factor although this phase is a necessary aspect of the wound healing process. Inflammation, driven by infiltrated immune cells begins minutes after the burn injury and is the prevalent phase of wound healing in the early stages. Accompanying the inflammatory infiltrate there is evidence that sub-populations of bone marrow derived cells are also present. These populations include fibrocytes and keratinocyte-like cells, derivatives of CD14+ monocytes a component of the peripheral blood mononuclear cell infiltrate. There is evidence that these cells contribute to regeneration and repair of the wound site but interestingly there are also reports that these cells can have adverse effects and may contribute to the development of dermal fibrosis. We present here a review of the transdifferentiation and origin of these cells from bone marrow stem cells, the environments that direct this transdifferentiation and evidence to support their role in fibrosis as well as potential avenues for therapeutics to control their fibrotic effects.

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Potential of Human Bone Marrow Stromal Cells to Accelerate Wound Healing in Vitro

Cited by 72 publications

References 36 publications

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

Therapeutic Potential of Gingival Fibroblasts for Cutaneous Radiation Syndrome: Comparison to Bone Marrow-Mesenchymal Stem Cell Grafts

Skin Tissue-Engineering Constructs and Stem Cells Application for the Skin Equivalents Creation (Review)

Fibrocyte and Keratinocyte-Like Cells and Their Role in Burn Wound Healing

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