Collagen scaffolds derived from bovine skin loaded with<scp>MSC</scp>optimized<scp>M1</scp>macrophages remodeling and chronic diabetic wounds healing

Liu, Hengdeng; Yang, Ronghua; Zhao, Shixin; Zhou, Fei; Liu, Yiling; Zhou, Ziheng; Chen, Lei; Xie, Julin

doi:10.1002/btm2.10467

Cited by 7 publications

(6 citation statements)

References 63 publications

(109 reference statements)

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“…MSCs can promote the basic functions of macrophages and mediate the polarization of macrophages phenotypes. Macrophages can secrete different active factors to regulate the immune microenvironment and affect the biological functions of MSCs through their own polarization (Figure 1 )[ 4 , 21 - 43 ].…”

Section: Bidirectional Interaction Between Mscs and Macrophagesmentioning

confidence: 99%

“…In addition, macrophages also play a certain regulatory role in the proliferation ability of MSCs. IL-1β, IL-6, TNF-α, and IFN-γ secreted by M1 macrophages can inhibit the proliferation of MSCs, while IL-10, TGF-β1, TGF-β3, and VEGF secreted by M2 macrophages can promote the proliferation of MSCs[ 4 , 5 ].…”

Section: The Effects Of Macrophages On Mscsmentioning

confidence: 99%

“…On the one hand, as pluripotent stem cells, MSCs have the abilities to self-renew and differentiate into multiple lineages. Under the influence of injury signals and chemokines, MSCs migrate to the site of injury, differentiate directionally, and secrete various growth factors, such as transforming growth factor-beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor, stromal cell-derived factor-1 (SDF-1), and fibroblast growth factor, to promote cell homing and stimulate tissue regeneration[ 4 , 5 ]. Factors in the injured environment, such as interferon gamma (IFN-γ) and tumor necrosis factor-α (TNF-α), stimulate the multilineage differentiation potential of MSCs, including osteogenic, adipogenic, and angiogenic differentiation, forming the various cells required for tissue repair[ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, as the first line of defense of the innate immune system, MSCs maintain the stability of the tissue microenvironment by controlling the inflammatory cascade[ 6 ]. MSCs can be activated by biomarkers in the inflammatory environment and respond to these signals through homing or paracrine effects, secreting soluble factors, such as prostaglandin E2 (PGE2), TGF-β1, indoleamine 2,3-dioxygenase (IDO), interleukin (IL)-6, and IL-10, as well as proteins and RNAs that control macrophages differentiation and promote the anti-inflammatory phenotypic transformation of macrophages to inhibit uncontrolled immune responses[ 4 , 5 , 7 - 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Interplay between mesenchymal stem cells and macrophages: Promoting bone tissue repair

Zhang,

Hao,

Zhang

et al. 2024

World J Stem Cells

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The repair of bone tissue damage is a complex process that is well-orchestrated in time and space, a focus and difficulty in orthopedic treatment. In recent years, the success of mesenchymal stem cells (MSCs)-mediated bone repair in clinical trials of large-area bone defects and bone necrosis has made it a candidate in bone tissue repair engineering and regenerative medicine. MSCs are closely related to macrophages. On one hand, MSCs regulate the immune regulatory function by influencing macrophages proliferation, infiltration, and phenotype polarization, while also affecting the osteoclasts differentiation of macrophages. On the other hand, macrophages activate MSCs and mediate the multilineage differentiation of MSCs by regulating the immune microenvironment. The cross-talk between MSCs and macrophages plays a crucial role in regulating the immune system and in promoting tissue regeneration. Making full use of the relationship between MSCs and macrophages will enhance the efficacy of MSCs therapy in bone tissue repair, and will also provide a reference for further application of MSCs in other diseases.

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Section: Bidirectional Interaction Between Mscs and Macrophagesmentioning

confidence: 99%

Section: The Effects Of Macrophages On Mscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interplay between mesenchymal stem cells and macrophages: Promoting bone tissue repair

Zhang,

Hao,

Zhang

et al. 2024

World J Stem Cells

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“…It is generally extracted and purified from various animal sources for use in biological applications [5]. Several gelatinous scaffold biomaterials derived from animal-derived collagen have been developed for wound healing, tissue homeostasis and bioengineering of various tissues including bone, cartilage, skin, cardiac tissue and others [6][7][8]. While there are several animal and fish sources utilized to extract collagen, the most common species employed is cattle (bovine) [9].…”

Section: Introductionmentioning

confidence: 99%

Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study

Goncalves,

Heise,

Banerjee

2023

Biomimetics

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Development of biocomposite scaffolds has gained tremendous attention due to their potential for tissue regeneration. However, most scaffolds often contain animal-derived collagen that may elicit an immunological response, necessitating the development of new biomaterials. Herein, we developed a new collagen-like peptide,(Pro-Ala-His)10 (PAH)10, and explored its ability to be utilized as a functional biomaterial by incorporating it with a newly synthesized peptide-based self-assembled gel. The gel was prepared by conjugating a pectin derivative, galataric acid, with a pro-angiogenic peptide (LHYQDLLQLQY) and further functionalized with a cortistatin-derived peptide, (Phe-Trp-Lys-Thr)4 (FWKT)4, and the bio-ionic liquid choline acetate. The self-assembly of (PAH)10 and its interactions with the galactarate-peptide conjugates were examined using replica exchange molecular dynamics (REMD) simulations. Results revealed the formation of a multi-layered scaffold, with enhanced stability at higher temperatures. We then synthesized the scaffold and examined its physicochemical properties and its ability to integrate with aortic smooth muscle cells. The scaffold was further utilized as a bioink for bioprinting to form three-dimensional cell-scaffold matrices. Furthermore, the formation of actin filaments and elongated cell morphology was observed. These results indicate that the (PAH)10 hybrid scaffold provides a suitable environment for cell adhesion, proliferation and growth, making it a potentially valuable biomaterial for tissue engineering.

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Multifunctional Bioactive Nanozyme Systems for Enhanced Diabetic Wound Healing

Gao,

He,

Liu

et al. 2024

Adv Healthcare Materials

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The protracted transition from inflammation to proliferation in diabetic wound healing poses significant challenges, exacerbated by persistent inflammatory responses and inadequate vascularization. To address these issues, a novel nanozymatic therapeutic approach utilizing asymmetrically structured MnO₂–Au–mSiO₂@aFGF Janus nanoparticles is engineered. Nanozymes featuring a mSiO₂ head and MnO₂ extensions, into which acidic fibroblast growth factor (aFGF) is encapsulated, resulting in MnO₂–Au–mSiO₂@aFGF Janus nanoparticles (mSAM@aFGF), are synthesized. This nanozyme system effectively emulates enzymatic activities of catalase (CAT) and superoxide dismutase (SOD), catalyzing degradation of reactive oxygen species (ROS) and generating oxygen. In addition, controlled release of aFGF fosters tissue regeneration and vascularization. In vitro studies demonstrate that mSAM@aFGF significantly alleviates oxidative stress in cells, and enhances cell proliferation, migration, and angiogenesis. An injectable hydrogel based on photocrosslinked hyaluronic acid (HAMA), incorporating the nanozymatic ROS‐scavenging and growth factor‐releasing system, is developed. The HAMA‐mSAM@aFGF hydrogel exhibits multifaceted benefits in a diabetic wound model, including injectability, wound adhesion, hemostasis, anti‐inflammatory effects, macrophage polarization from M1 to M2 phenotype, and promotion of vascularization. These attributes underscore the potential of this system to facilitate transition from chronic inflammation to the proliferative phase of wound repair, offering a promising therapeutic strategy for diabetic wound management.

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Collagen scaffolds derived from bovine skin loaded withMSCoptimizedM1macrophages remodeling and chronic diabetic wounds healing

Cited by 7 publications

References 63 publications

Interplay between mesenchymal stem cells and macrophages: Promoting bone tissue repair

Interplay between mesenchymal stem cells and macrophages: Promoting bone tissue repair

Development of Self-Assembled Biomimetic Nanoscale Collagen-like Peptide-Based Scaffolds for Tissue Engineering: An In Silico and Laboratory Study

Multifunctional Bioactive Nanozyme Systems for Enhanced Diabetic Wound Healing

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