Collagen precipitation from solutions

Vasil'ev, M.P.; Kotetskii, V. V.; Pukhova, Z. I.; Vol'f, L. A.; Истранов, Л. П.

doi:10.1007/bf00548873

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“…We have observed that other brands of collagen (even those labeled as Rat tail, Type I) have different viscosities or may come at a different stock concentration. In the latter case, diluting the collagen to a more workable concentration may be difficult requiring even mixing to generate a homogenous solution and having a non-precise pH may also result in the collagen crashing out of solution ( Vasilev et al., 1973 ).…”

Section: Troubleshootingmentioning

confidence: 99%

Mass production of human airway-like neutrophils via transmigration in an organotypic model of human airways

et al. 2021

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Summary Neutrophils are difficult to study, particularly in tissues, due to their short half-life and propensity for activation. We describe an organotypic airway model that uses patient airway fluid to enable the transmigration of blood neutrophils to acquire an airway-like phenotype in order to better understand their contribution to airway diseases. In particular, we showcase how conditioned neutrophils modulate their bacteria-killing abilities. For complete details on the use and execution of this protocol, please refer to Margaroli et al. (2021) .

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Section: Troubleshootingmentioning

confidence: 99%