Collagen IV of basement membranes: IV. Adaptive mechanism of collagen IV scaffold assembly in Drosophila

Summers, Jacob A.; Yarbrough, Madison; Liu, Min; McDonald, W. Hayes; Hudson, Billy G.; Pastor-Pareja, José C.; Boudko, Sergei P.

doi:10.1016/j.jbc.2023.105394

Journal of Biological Chemistry

2023

DOI: 10.1016/j.jbc.2023.105394

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Collagen IV of basement membranes: IV. Adaptive mechanism of collagen IV scaffold assembly in Drosophila

Jacob A. Summers,

Madison Yarbrough,

Min Liu

et al.

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2024

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Cited by 2 publications

References 100 publications

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Peroxidasin is required for full viability in development and for maintenance of tissue mechanics in adults

Peebles,

LaFever,

Page-McCaw

et al. 2024

Matrix Biology

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Peroxidasin is required for full viability in development and for maintenance of tissue mechanics in adults

Peebles,

LaFever,

Page-McCaw

et al. 2024

Matrix Biology

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Linking Basement Membrane and Slit Diaphragm in Drosophila Nephrocytes

Leroy,

Lang,

Spitz

et al. 2024

JASN

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Background: The glomerular basement membrane and the slit diaphragm are essential parts of the filtration barrier. How these layers collaborate remains unclear. The podocyte-like nephrocytes in Drosophila harbor both a slit diaphragm and a basement membrane, serving as a model to address this critical question. Methods: Basement membrane components and matrix receptors were silenced using RNAi in nephrocytes. Slit diaphragms were analyzed by immunofluorescence followed by automated quantification. Tracer endocytosis was applied for functional readouts. Results: Immunofluorescence indicated a significant reduction of slit diaphragm density upon loss of laminin and collagen IV components. This was accompanied by reduced expression of fly nephrin and shallower membrane invaginations. Tracer studies revealed that the basement membrane defines properties of the nephrocyte filtration barrier. Acute enzymatic disruption of the basement membrane via collagenase rapidly caused slit diaphragm mislocalization and disintegration, which was independent of cell death. Loss of matrix-interacting receptors, particularly integrins mys and mew, phenocopied basement membrane disruption. Integrins and nephrin co-localized at the slit diaphragm in nephrocytes in a mutually dependent manner, interacting genetically. Human integrin α3 interacted physically with nephrin. Conclusions: The glomerular basement membrane model in Drosophila nephrocytes reveals that matrix receptor-mediated cues ensure correct positioning of the slit diaphragm and the overall filtration barrier architecture.

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Collagen IV of basement membranes: IV. Adaptive mechanism of collagen IV scaffold assembly in Drosophila

Cited by 2 publications

References 100 publications

Peroxidasin is required for full viability in development and for maintenance of tissue mechanics in adults

Peroxidasin is required for full viability in development and for maintenance of tissue mechanics in adults

Linking Basement Membrane and Slit Diaphragm in Drosophila Nephrocytes

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