Collagen/hydroxyapatite composite nanofibers by electrospinning

Teng, Sheng; Lee, Eunjung; Wang, Peng; Kim, Hyoun‐Ee

doi:10.1016/j.matlet.2008.01.104

Cited by 91 publications

(67 citation statements)

References 12 publications

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“…At least five possible biological responses have been suggested after implantation of extra cellular matrixes including (i) ECM non-incorporating responses: (a) encapsulation; (b) rejection; and (ii) ECM-incorporating responses: (a) resorption; (b) integration with progressive degradation; (c) adoption and adaptation 7,8,37,44 . Several attempts have been made to improve the biological behaviour of the scaffolds with the aim of improving their biocompatibility, biodegradability and bioefficacy and decreasing the rejection rate 7,39,44,51,59 . Acellularization or decellularization is the first step in this issue, especially in those biologically based tissue-engineered grafts obtained from allo-or xenografts 7,9,33,51 .…”

mentioning

confidence: 99%

“…By removing all cellular and microbiological structures, the immune response could be reduced in order to increase the chance of graft incorporation in the healing process 15,33,34 . Increasing the number of specified molecules in the architecture of the scaffold could reduce the limitation of each molecule 15,59 . The best-suited scaffold for tendon tissue engineering should have the following biological behaviour 44 : it should not acutely or chronically be rejected by the host immune response and should not be encapsulated by fibrous connective tissue 44 .…”

mentioning

confidence: 99%

See 1 more Smart Citation

Role of tissue engineering in tendon reconstructive surgery and regenerative medicine: Current concepts, approaches and concerns

Moshiri¹,

Oryan²

2012

Hard Tissue

193

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mentioning

confidence: 99%

mentioning

confidence: 99%

Role of tissue engineering in tendon reconstructive surgery and regenerative medicine: Current concepts, approaches and concerns

Moshiri¹,

Oryan²

2012

Hard Tissue

193

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“…This technique can be used to fabricate an interconnected porous structure, which can ensure a suitable local environment for artificial bone applications [11,14]. The interconnected porous structure of the ZrO 2 /BCP scaffold was fabricated using the sponge replica method.…”

Section: Discussionmentioning

confidence: 99%

“…The sponge replica method has been widely used for bone scaffolds because it can produce a highly porous interconnected structure that mimics the cancellous bone [11,12]. Electrospinning is an alternative technique that can be used to produce fibrous scaffolds with a structure similar to that of the extracellular matrix [13,14]. These scaffolds have a porous structure and the diameter of the constituting fibers can be controlled from the nano-to micrometer scale.…”

Section: Introductionmentioning

confidence: 99%

Novel approach to the fabrication of an artificial small bone using a combination of sponge replica and electrospinning methods

Kim

Lee

2011

Science and Technology of Advanced Materials

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In this study, a novel artificial small bone consisting of ZrO 2 -biphasic calcium phosphate/polymethylmethacrylate-polycaprolactone-hydroxyapatite (ZrO 2 -BCP/PMMA-PCL-HAp) was fabricated using a combination of sponge replica and electrospinning methods. To mimic the cancellous bone, the ZrO 2 /BCP scaffold was composed of three layers, ZrO 2 , ZrO 2 /BCP and BCP, fabricated by the sponge replica method. The PMMA-PCL fibers loaded with HAp powder were wrapped around the ZrO 2 /BCP scaffold using the electrospinning process. To imitate the Haversian canal region of the bone, HAp-loaded PMMA-PCL fibers were wrapped around a steel wire of 0.3 mm diameter. As a result, the bundles of fiber wrapped around the wires imitated the osteon structure of the cortical bone. Finally, the ZrO 2 /BCP scaffold was surrounded by HAp-loaded PMMA-PCL composite bundles. After removal of the steel wires, the ZrO 2 /BCP scaffold and bundles of HAp-loaded PMMA-PCL formed an interconnected structure resembling the human bone. Its diameter, compressive strength and porosity were approximately 12 mm, 5 MPa and 70%, respectively, and the viability of MG-63 osteoblast-like cells was determined to be over 90% by the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. This artificial bone shows excellent cytocompatibility and is a promising bone regeneration material.

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“…54,55 In addition, most of the electrospinning solutions (or dispersions) were prepared by a conventional mixing method in which HAP was normally obtained firstly in powders and followed by mixing it with collagen solutions. [40][41][42]56 Consequently, in the prepared collagen/HAP composites, the particle size of HAP was not uniform and the HAP aggregation was often randomly distributed within the collagen matrix. Such a composite was obviously different from the natural bone microstructure, and only had a compositional similarity to that of natural bone.…”

Section: Zhou Et Almentioning

confidence: 99%

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

Zhou¹,

Yao²,

Wang³

et al. 2015

IJN

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In bone tissue engineering, collagen/hydroxyapatite (HAP) fibrous composite obtained via electrospinning method has been demonstrated to support the cells’ adhesion and bone regeneration. However, electrospinning of natural collagen often requires the use of cytotoxic organic solvents, and the HAP crystals were usually aggregated and randomly distributed within a fibrous matrix of collagen, limiting their clinical potential. Here, an effective and greener method for the preparation of collagen/HAP composite fibers was developed for the first time, and this green product not only had 40 times higher mechanical properties than that previously reported, but also had an excellent microstructure similar to that of natural bone. By dissolving type I collagen in environmentally friendly phosphate buffered saline/ethanol solution instead of the frequently-used cytotoxic organic solvents, followed with the key step of desalination, co-electrospinning the collagen solution with the HAP sol, generates a collagen/HAP composite with a uniform and continuous fibrous morphology. Interestingly, the nano-HAP needles were found to preferentially orient along the longitudinal direction of the collagen fibers, which mimicked the nanostructure of natural bones. Based on the characterization of the related products, the formation mechanism for this novel phenomenon was proposed. After cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/ N -hydroxysuccinimide, the obtained composite exhibited a significant enhancement in mechanical properties. In addition, the biocompatibility of the obtained composite fibers was evaluated by in vitro culture of the human myeloma cells (U2-OS). Taken together, the process outlined herein provides an effective, non-toxic approach for the fabrication of collagen/HAP composite nanofibers that could be good candidates for bone tissue engineering.

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Collagen/hydroxyapatite composite nanofibers by electrospinning

Cited by 91 publications

References 12 publications

Role of tissue engineering in tendon reconstructive surgery and regenerative medicine: Current concepts, approaches and concerns

Role of tissue engineering in tendon reconstructive surgery and regenerative medicine: Current concepts, approaches and concerns

Novel approach to the fabrication of an artificial small bone using a combination of sponge replica and electrospinning methods

Greener synthesis of electrospun collagen/hydroxyapatite composite fibers with an excellent microstructure for bone tissue engineering

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