Collagen hydrolysate intake improves the loss of epidermal barrier function and skin elasticity induced by <scp>UVB</scp> irradiation in hairless mice

Oba, Chisato; Ohara, Hiroki; Morifuji, Masashi; Ito, Kyoko; Ichikawa, Satomi; Kawahata, Keiko; Koga, Jinichiro

doi:10.1111/phpp.12051

Cited by 58 publications

(51 citation statements)

References 29 publications

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“…On the other hand, several reports have shown that treatment with CP ingestion only significantly improves epidermal thickening and impairment of skin elasticity in UVB-irradiated hairless mice. [26][27][28] These data indicate that treatment with CP only discriminates rescue effects between hairless Sod1 −/− and UVB-irradiated hairless wild-type mice. In fact, Sod1 −/− fibroblasts exhibit marked loss of cell viability and a significant increase in apoptotic cell death associated with a 10-fold increase in intracellular reactive oxygen species (ROS) generation.…”

Section: Discussionmentioning

confidence: 78%

Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice

Shibuya

Ozawa

Toda

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Age-related skin thinning is correlated with a decrease in the content of collagen in the skin. Accumulating evidence suggests that collagen peptide (CP) and vitamin C (VC) transcriptionally upregulate type I collagen in vivo. However, the additive effects of CP and VC on age-related skin changes remain unclear. We herein demonstrate that CP and a VC derivative additively corrected age-related skin thinning via reduced oxidative damage in superoxide dismutase 1 (Sod1)-deficient mice. Co-treatment with these compounds significantly normalized the altered gene expression of Col1a1, Has2, and Ci1, a proton-coupled oligopeptide transporter, in Sod1−/− skin. The in vitro analyses further revealed that collagen oligopeptide, a digestive product of ingested CP, significantly promoted the bioactivity of the VC derivative with respect to the migration and proliferation of Sod1−/− fibroblasts. These findings suggest that combined treatment with CP and VC is effective in cases of age-related skin pathology.

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Section: Discussionmentioning

confidence: 78%

Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice

Shibuya

Ozawa

Toda

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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“…Fish scale collagen hydrolysate was purchased from Nitta Gelatin (Osaka, Japan). This amount of collagen hydrolysate is approximately equivalent to oral ingestion of 2.0 g collagen hydrolysate/kg body wt/day (32). The composition of the experimental diets is shown in Table 1.…”

Section: Experiments 1 (Skin Evaluation In An Aging Mouse Model)mentioning

confidence: 99%

“…Stratum corneum water content and skin elasticity were assessed under standardized conditions (external temperature 24 Ϯ 1°C and humidity 50 Ϯ 10%). Stratum corneum water content and skin elasticity were measured with a SKICON 200-EX skin surface hygrometer (I.B.S., Shizuoka, Japan) and a Cutometer SEM575 (Courage and Khazaka Electronic, Cologne, Germany), as described previously (32). The kinetics of skin displacement (2 mm diameter probe) was measured over a 2 s interval in response to a 300 mbar suction, followed by a 2 s relaxation period after termination of the suction.…”

Section: Experiments 1 (Skin Evaluation In An Aging Mouse Model)mentioning

confidence: 99%

“…Dietary supplementation with food ingredients, such as vitamins and polyphenols, helps modulate skin function (4,18,47). We have also reported that repetitive collagen hydrolysate administration improved the loss of epidermal barrier function and skin elasticity in hairless mice following a single dose or repeated doses of ultraviolet B (UV-B) irradiation (32). DNA microarray technology makes it possible to analyze comprehensively the expression of many genes and to find several novel markers associated with physiological functions.…”

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confidence: 99%

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Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis

Oba¹,

Ito²,

Ichikawa³

et al. 2015

Physiological Genomics

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Oba C, Ito K, Ichikawa S, Morifuji M, Nakai Y, Ishijima T, Abe K, Kawahata K. Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis. Physiol Genomics 47: [355][356][357][358][359][360][361][362][363] 2015. First published June 9, 2015; doi:10.1152/physiolgenomics.00009.2015.-Dietary collagen hydrolysate has been hypothesized to improve skin barrier function. To investigate the effect of long-term collagen hydrolysate administration on the skin, we evaluated stratum corneum water content and skin elasticity in intrinsically aged mice. Female hairless mice were fed a control diet or a collagen hydrolysate-containing diet for 12 wk. Stratum corneum water content and skin elasticity were gradually decreased in chronologically aged control mice. Intake of collagen hydrolysate significantly suppressed such changes. Moreover, we used DNA microarrays to analyze gene expression in the skin of mice that had been administered collagen hydrolysate. Twelve weeks after the start of collagen intake, no significant differences appeared in the gene expression profile compared with the control group. However, 1 wk after administration, 135 genes were upregulated and 448 genes were downregulated in the collagen group. This suggests that gene changes preceded changes of barrier function and elasticity. We focused on several genes correlated with functional changes in the skin. Gene Ontology terms related to epidermal cell development were significantly enriched in upregulated genes. These skin function-related genes had properties that facilitate epidermal production and differentiation while suppressing dermal degradation. In conclusion, our results suggest that altered gene expression at the early stages after collagen administration affects skin barrier function and mechanical properties. Long-term oral intake of collagen hydrolysate improves skin dysfunction by regulating genes related to production and maintenance of skin tissue. collagen hydrolysate; skin aging; epidermal barrier function; skin elasticity; DNA microarray SKIN AGING IS MAINLY CLASSIFIED into two categories: chronological aging and photoaging. Chronological aging, or so-called "physiological aging," indicates natural age-induced intrinsic changes. Chronologically aged skin is characterized by dry skin, atrophy, laxity, and wrinkling. As skin ages, the rate of cell division of keratinocytes slows down and the reduced cell number induces epidermal thinning and flattening of the epidermal-dermal junction (7, 31) and results in decreased epidermal thickness (17). Loss of natural moisture factors and intercellular lipids such as ceramide induce dry skin, and there is a reduction of epidermal barrier function (13, 39). Additionally, fibroblast dysfunction and extracellular matrix (ECM) alterations appear in the dermis with aging. Decreases in the content of glycosaminoglycan, proteoglycans, and degeneration of collagenous and elastic fibers induce a decline in skin elasticity, wrinkle formation, and sa...

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“…6) Collagen hydrolysate (CH) also improves the skin moisture content 7) and skin elasticity. 8) In this study, we describe the pharmacological effects of a dietary herb extract (HE) from E. ulmoides leaves and Korean ginseng, and a combination of HE and CH, on hairless mouse skin under repeated UV irradiation.…”

mentioning

confidence: 99%

Herb extracts and collagen hydrolysate improve skin damage resulting from ultraviolet-induced aging in hairless mice

Jimbo

Kawada

Nomura

2015

Bioscience, Biotechnology, and Biochemistry

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We examined the effect of the daily ingestion of herb extract from Eucommia ulmoides leaves and Korean ginseng on skin damage induced by repeated UV irradiation of hairless mice. The herb extract was orally administered to mice at a dose of 1000 mg/kg/day. The hydration of mice dorsal skin decreased significantly with repeated UV irradiation, but did not decrease when the herb extract was administered for seven weeks. Transepidermal water loss (TEWL) increased with UV irradiation, but decreased with the administration of dietary herb extract. These effects were more pronounced when combined with the administration of collagen hydrolysate. Geniposidic acid from E. ulmoides leaves and ginsenoside Rg1 from Korean ginseng reduced TEWL and increased the skin moisture content of UV-damaged skin on hairless mice, respectively. We concluded that this dietary herb extract reduced the skin damage caused by UV-induced aging, with geniposidic acid and ginsenoside Rg1 detected in the blood.

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Collagen hydrolysate intake improves the loss of epidermal barrier function and skin elasticity induced by UVB irradiation in hairless mice

Abstract: Collagen hydrolysate administration affects epidermal barrier function and dermal skin elasticity.

Cited by 58 publications

References 29 publications

Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice

Collagen peptide and vitamin C additively attenuate age-related skin atrophy in Sod1-deficient mice

Effect of orally administered collagen hydrolysate on gene expression profiles in mouse skin: a DNA microarray analysis

Herb extracts and collagen hydrolysate improve skin damage resulting from ultraviolet-induced aging in hairless mice

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