Collagen grafted 3D polycaprolactone scaffolds for enhanced cartilage regeneration

Cai, Yanli; Li, Jinlan; Poh, Chye Khoon; Tan, Hark Chuan; Thian, Eng San; Fuh, Jerry Ying Hsi; Sun, Junying; Tay, B.Y.; Wang, Wilson

doi:10.1039/c3tb20680g

Cited by 55 publications

(33 citation statements)

References 27 publications

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“…After surface functionalization with polydopamine and collagen, aggregates of polydopamine particles were observed to distribute on the filaments, and the presence of N 1s in the X‐ray spectroscopy scan suggested the grafting of collagen on the scaffold. In addition, the PCL scaffold became more hydrophilic …”

Section: Methodsmentioning

confidence: 99%

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Fabrication of three‐dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E‐jetting technique

Cai

Guo

et al. 2013

J Biomed Mater Res

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Biodegradable polymeric scaffolds have been widely used in tissue engineering as a platform for cell proliferation and subsequent tissue regeneration. Conventional microextrusion methods for three-dimensional (3D) scaffold fabrication were limited by their low resolution. Electrospinning, a form of electrohydrodynamic (EHD) printing, is an attractive method due to its capability of fabricating high-resolution scaffolds at the nanometer/micrometer scale level. However, the scaffold was composed of randomly orientated filaments which could not guide the cells in a specific direction. Furthermore, the pores of the electrospun scaffold were small, thus preventing cell infiltration. In this study, an alternative EHD jet printing (E-jetting) technique has been developed and employed to fabricate 3D polycaprolactone (PCL) scaffolds with desired filament orientation and pore size. The effect of PCL solution concentration was evaluated. Results showed that solidified filaments were achieved at concentration >70% (w/v). Uniform filaments of diameter 20 μm were produced via the E-jetting technique, and X-ray diffraction and attenuated total reflectance Fourier transform infrared spectroscopic analyses revealed that there was no physicochemical changes toward PCL. Scaffold with a pore size of 450 μm and porosity level of 92%, was achieved. A preliminary in vitro study illustrated that live chondrocytes were attaching on the outer and inner surfaces of collagen-coated E-jetted PCL scaffolds. E-jetted scaffolds increased chondrocytes extracellular matrix secretion, and newly formed matrices from chondrocytes contributed significantly to the mechanical strength of the scaffolds. All these results suggested that E-jetting is an alternative scaffold fabrication technique, which has the capability to construct 3D scaffolds with aligned filaments and large pore sizes for tissue engineering applications.

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Section: Methodsmentioning

confidence: 99%

“…In addition, the PCL scaffold became more hydrophilic. 23 Scaffolds were then placed into a 24-well plate, and 50 ml of cell aliquot was seeded at the density of 4 3 10 5 cells cm 22 . Cells seeded on 24-well polystyrene culture dishes (1 3 10 5 cells cm 22 ) were used as controls.…”

Section: Scaffold Characterizationmentioning

confidence: 99%

Fabrication of three‐dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E‐jetting technique

Cai

Guo

et al. 2013

J Biomed Mater Res

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“…Electrohydrodynamic Jetting, which is also known as EHD-Jetting or E-jetting is one type of bioprinting technology. E-jetting has the same working principle as Electrospinning technique which is widely used to fabricate controlled porous scaffolds for tissue engineering applications [21][22][23][24][25][26] . Various studies were made on the effect of Electrospinning parameters on the electrospun fibres [27][28] .…”

Section: Introductionmentioning

confidence: 99%

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Wang¹,

Vijayavenkataraman²,

Wu³

et al. 2016

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Abstract:Tissue engineering is a promising technology in the field of regenerative medicine with its potential to create tissues de novo. Though there has been a good progress in this field so far, there still exists the challenge of providing a 3D micro-architecture to the artificial tissue construct, to mimic the native cell or tissue environment. Both 3D printing and 3D bioprinting are looked upon as an excellent solution due to their capabilities of mimicking the native tissue architecture layer-by-layer with high precision and appreciable resolution. Electrohydrodynamic jetting (E-jetting) is one type of 3D printing, in which, a high electric voltage is applied between the extruding nozzle and the substrate in order to print highly controlled fibres. In this study, an E-jetting system was developed in-house for the purpose of 3D printing of fibrous scaffolds. The effect of various E-jetting parameters, namely the supply voltage, solution concentration, nozzle-to-substrate distance, stage (printing) speed and solution dispensing feed rate on the diameter of printed fibres were studied at the first stage. Optimized parameters were then used to print Polycaprolactone (PCL) scaffolds of highly complex geometries, i.e., semi-lunar and spiral geometries, with the aim of demonstrating the flexibility and capability of the system to fabricate complex geometry scaffolds and biomimic the complex 3D micro-architecture of native tissue environment. The spiral geometry is expected to result in better cell migration during cell culture and tissue maturation.

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“…Reproduced with permission. [ 214 ] Copyright 2013, The Royal Society of Chemistry. e) High resolution 3D scaffold fabrication by electrohydrodynamic jet printing at elevated temperatures from melted PCL.…”

Section: Directing Cell-substrate Interactionsmentioning

confidence: 98%

Mechanisms, Capabilities, and Applications of High‐Resolution Electrohydrodynamic Jet Printing

Önses

Sutanto

Ferreira

et al. 2015

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This review gives an overview of techniques used for high-resolution jet printing that rely on electrohydrodynamically induced flows. Such methods enable the direct, additive patterning of materials with a resolution that can extend below 100 nm to provide unique opportunities not only in scientific studies but also in a range of applications that includes printed electronics, tissue engineering, and photonic and plasmonic devices. Following a brief historical perspective, this review presents descriptions of the underlying processes involved in the formation of liquid cones and jets to establish critical factors in the printing process. Different printing systems that share similar principles are then described, along with key advances that have been made in the last decade. Capabilities in terms of printable materials and levels of resolution are reviewed, with a strong emphasis on areas of potential application.

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Collagen grafted 3D polycaprolactone scaffolds for enhanced cartilage regeneration

Cited by 55 publications

References 27 publications

Fabrication of three‐dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E‐jetting technique

Fabrication of three‐dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E‐jetting technique

Investigation of process parameters of electrohydrodynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries

Mechanisms, Capabilities, and Applications of High‐Resolution Electrohydrodynamic Jet Printing

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