Collagen fibers as a temporary scaffold for replacement of ACL in goats

Chvapil, Miloš; Speer, Donald P.; Holubec, Hana; Chvapil, Thomas A.; King, D. H.

doi:10.1002/jbm.820270305

Cited by 79 publications

(36 citation statements)

References 15 publications

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“…3,5,8,9 When these scaffolds were implanted in animal models, they demonstrated some success in promoting neotendon and neoligament regeneration. 5,10,11 However, this success has been limited both by insufficient tissue ingrowth from the wound margin and by initial mechanical properties that do not adequately match the native tissue properties regardless of the chemical or physical crosslinking treatment used to stabilize the scaffold. 8,10,11 While these crosslinking strategies improve the strength and stiffness of the material, they may alter the scaffold surface biochemistry in a manner that impedes cellular infiltration and the rate of new tissue ingrowth.…”

Section: Introductionmentioning

confidence: 99%

“…5,10,11 However, this success has been limited both by insufficient tissue ingrowth from the wound margin and by initial mechanical properties that do not adequately match the native tissue properties regardless of the chemical or physical crosslinking treatment used to stabilize the scaffold. 8,10,11 While these crosslinking strategies improve the strength and stiffness of the material, they may alter the scaffold surface biochemistry in a manner that impedes cellular infiltration and the rate of new tissue ingrowth. 8 Consequently, there remains a need to create collagen thread scaffolds that enhance the rate of cell migration onto tissue analogs to increase the rate of new tissue ingrowth and maintain the mechanical stability of the scaffold.…”

Section: Introductionmentioning

confidence: 99%

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Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Cornwell

Lei

Andreadis

et al. 2006

J Biomedical Materials Res

133

111

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Bundles of threads extruded from type I collagen have been researched extensively as scaffolds to promote the repair and regeneration of torn tendons and ligaments. The success of these scaffolds has been limited by insufficient tissue ingrowth from the wound margin, which may be inhibited by the chemical or physical crosslinking treatment used to increase the mechanical properties and decrease the degradation rate of these scaffolds. Recently, self-assembled collagen threads extruded from solutions of type I collagen molecules were shown to possess ultimate tensile strengths and structural properties comparable to native tendon fibers; however the tissue response to these threads has yet to be determined. The goal of this study was to investigate the effects of various crosslinking techniques on the mechanical properties as well as the in vitro rate of new tissue ingrowth on these threads. Our findings indicate that the physical crosslinking techniques, dehydrothermal (DHT) or ultraviolet light (UV), most significantly improve the mechanical strengths of the threads, but most significantly decrease the rate of cell migration. In contrast, carbodiimide (EDC) crosslinking achieved sub-optimal strength generation, but demonstrated improved cell migration rates. Future studies will investigate the design of threads with surface biochemistries that maximize tissue ingrowth while maintaining the mechanical stability of the scaffold.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Crosslinking of discrete self‐assembled collagen threads: Effects on mechanical strength and cell–matrix interactions

Cornwell

Lei

Andreadis

et al. 2006

J Biomedical Materials Res

133

111

View full text Add to dashboard Cite

show abstract

“…Several groups have reported the potential construction of an ACL scaffold using a carbon copolymer, collagen, silk, and biodegradable polymers, such as polyglycolic acid (PGA) and polylactic acid (PLA). [9][10][11][12][13][14][15] The use of composite ACL prostheses, the union of a collagen inductor and a synthetic fiber (Dacron 1 ), was investigated as a possible means of ACL reconstruction. Composite ACL prostheses were implanted in 10 sheep models for 6 months.…”

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confidence: 99%

“…11 Therefore, a natural polymer, collagen, was used as a scaffold material in an attempt to reduce the inflammatory reaction. Chvapil et al 12 attempted ACL reconstruction with tendon collagen. After the removal of noncollagenous proteins and glycosaminoglycan from the tendon, collagen fibers were implanted in the ruptured ACLs of goats.…”

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confidence: 99%

Increase in cell migration and angiogenesis in a composite silk scaffold for tissue‐engineered ligaments

Seo

Yoon

Song

et al. 2009

Journal Orthopaedic Research

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The purpose of this study was to evaluate the biocompatibility of silk and collagen-hyaluronan (HA) in vitro by assessing anterior cruciate ligament (ACL) cell and T-lymphocyte cultures on scaffolds. The use of composite scaffolds as artificial ligaments in ACL reconstruction and their effects on angiogenesis were evaluated in vivo. The silk scaffold was knitted by hand and dry coated with collagen-HA, whereas the composite silk scaffold was made by covering a silk scaffold with a lyophilized collagen-HA substrate. The initial attachment and proliferation of human ACL cells on the composite silk scaffold was superior to the attachment and proliferation observed on the silk scaffold. The immune response was higher in both scaffolds after 72 h ( p < 0.05) compared with the control culture condition without scaffolding, as assessed by T-lymphocyte cultures in vitro. There was no significant difference in the immune response in vitro between the silk and composite silk scaffolds. Silk and composite silk scaffolds were implanted as artificial ligaments in ACLs removed from the knees of dogs, and they were harvested 6 weeks after implantation. On gross examination, the onset of an inflammatory tissue reaction, such as synovitis, was seen in both the silk scaffold and the composite silk scaffold groups. An histological evaluation of the artificial ligament implants revealed the presence of monocytes in the silk composite scaffold and the absence of giant cells in all cases. MT staining in the composite silk scaffold-grafted group showed granulation tissue consisting of fibroblasts, lymphocytes, monocytes, and collagen fibers. In addition, CD31 staining revealed the formation of new blood vessels. On the other hand, no reparative tissues, such as blood vessels, collagen, and cells, were observed in the silk scaffold-grafted group. These results suggest that the lyophilized collagen-HA substrate is biocompatible in vitro and enhances new blood vessel and cell migration in vivo. ß

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“…Implanted into goats, analysed over 11 months post implantation, concluded they were loosing strength, therefore not suitable as ACL substitute. (Chvapil, Speer et al 1993) Table 5. Some of the various different materials previously used as scaffolds (for ligament tissue engineering) their physical forms, and their suitability for cell cultures…”

Section: Tissue Engineering For Tissue and Organ Regeneration 144mentioning

confidence: 99%