Collagen Fibers and Inflammatory Cells in Healthy and Diseased Human Gingival Tissues: A Comparative and Quantitative Study by Immunohistochemistry and Automated Image Analysis

Séguier, Sylvie; Godeau, Gaston; Brousse, Nicole

doi:10.1902/jop.2000.71.7.1079

Cited by 60 publications

(45 citation statements)

References 33 publications

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“…Ramfjord [27] reported that collagen formation is a gradual process during the first 3-4 weeks after surgery and starts with a decline in initial vascularization, with the tissue already being practically mature at 35 days. In the present study, the number of collagen fibers was high in both groups throughout the period analyzed, occupying 80% of the connective tissue volume on average, a value relatively higher than the 50%-63% reported in the literature [32,33]. This difference might be due to the thickness of the histological sections used here (5 mm).…”

Section: Discussioncontrasting

confidence: 68%

Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low‐level laser therapy

et al. 2004

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Section: Discussioncontrasting

confidence: 68%

Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low‐level laser therapy

et al. 2004

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“…42,43 A representative photomicrograph of gingival tissue stained with Giemsa, demonstrating the presence of immune cells in the tissue, is presented in Figure 1A. The leukocyte marker CD45 (LCA, leukocyte common antigen) was used to assess the histological grade of inflammation, shown in double staining with H&E ( Figure 1B).…”

Section: Presence Of Cd45 and Il-1␤ In Gingival Tissue Of Patients Wimentioning

confidence: 99%

Expression of Prostaglandin E Synthases in Periodontitis

Båge

Kats

Lopez

et al. 2011

The American Journal of Pathology

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The inflammatory mediator prostaglandin E 2 (PGE 2 ) is implicated in the pathogenesis of chronic inflammatory diseases including periodontitis; it is synthesized by cyclooxygenases (COX) and the prostaglandin E synthases mPGES-1, mPGES-2, and cPGES. The distribution of PGES in gingival tissue of patients with periodontitis and the contribution of these enzymes to inflammationinduced PGE 2 synthesis in different cell types was investigated. In gingival biopsies, positive staining for PGES was observed in fibroblasts and endothelial, smooth muscle, epithelial, and immune cells. To further explore the contribution of PGES to inflammation-induced PGE 2 production, in vitro cell culture experiments were performed using fibroblasts and endothelial, smooth muscle, and mast cells. All cell types expressed PGES and COX-2, resulting in basal levels of PGE 2 synthesis. In response to tumor necrosis factor (TNF-␣), IL-1␤, and cocultured lymphocytes, however, mPGES-1 and COX-2 protein expression increased in fibroblasts and smooth muscle cells, accompanied by increased PGE 2 , whereas mPGES-2 and cPGES were unaffected. In endothelial cells, TNF-␣ increased PGE 2 production only via COX-2 expression, whereas in mast cells the cytokines did not affect PGE 2 enzyme expression or PGE 2 production. Furthermore, PGE 2 production was diminished in gingival fibroblasts derived from mPGES-1 knockout mice, compared with wild-type fibroblasts. These results suggest that fibroblasts and smooth muscle cells are important sources of mPGES-1, which may contribute to increased PGE 2 production in the inflammatory condition periodontitis.

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“…Sections (5 μm) were stained with hematoxylin and eosin and with sirius red F3Ba (Junqueira et al, 1979). Fibroblast numbers and mean area fraction of collagen were determined from 6 different fields in each individual, as described previously (Séguier et al, 2000;Ejeil et al, 2003). Sections observed with polarized light were imaged and analyzed with ImageLab software (Diracom, Vargem Grande do Sul, SP, Brazil), which performed transformations of mathematical morphology and calculation of the area fraction occupied by collagen.…”

Section: Histological and Quantitative Determination Of Collagen Areamentioning

confidence: 99%

Characterization of Fibroblasts with Son of Sevenless-1 Mutation

et al. 2006

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Although non-syndromic hereditary gingival fibromatosis (HGF) is genetically heterogeneous, etiologic mutations have been identified only in the Son of Sevenless-1 gene (SOS1). To test evidence of increased cell proliferation, we studied histological, morphological, and proliferation characteristics in monolayer and three-dimensional cultures of fibroblasts with the SOS1 g. 126,142-126,143insC mutation. Histological assessment of HGF gingiva indicated increased numbers of fibroblasts (30%) and increased collagen (10%). Cell proliferation studies demonstrated increased growth rates and 5-bromo-2-deoxyuridine incorporation for HGF fibroblasts. Flow cytometry showed greater proportions of HGF fibroblasts in the G2/M phase. Attachment of HGF fibroblasts to different extracellular matrix surfaces demonstrated increased formation of protrusions with lamellipodia. HGF fibroblasts in three-dimensional culture showed greater cell proliferation, higher cell density, and alteration of surrounding collagen matrix. These findings revealed that increased fibroblast numbers and collagen matrix changes are associated with mutation of the SOS1 gene in vitro and in vivo.

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Collagen Fibers and Inflammatory Cells in Healthy and Diseased Human Gingival Tissues: A Comparative and Quantitative Study by Immunohistochemistry and Automated Image Analysis

Cited by 60 publications

References 33 publications

Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low‐level laser therapy

Histomorphometric study of the healing of human oral mucosa after gingivoplasty and low‐level laser therapy

Expression of Prostaglandin E Synthases in Periodontitis

Characterization of Fibroblasts with Son of Sevenless-1 Mutation

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