Collagen-based silver nanoparticles for biological applications: synthesis and characterization

Abstract

BackgroundType I collagen is an abundant natural polymer with several applications in medicine as matrix to regenerate tissues. Silver nanoparticles is an important nanotechnology material with many utilities in some areas such as medicine, biology and chemistry. The present study focused on the synthesis of silver nanoparticles (AgNPs) stabilized with type I collagen (AgNPcol) to build a nanomaterial with biological utility. Three formulations of AgNPcol were physicochemical characterized, antibacterial activity in vitro and cell viability assays were analyzed. AgNPcol was characterized by means of the following: ultraviolet–visible spectroscopy, dynamic light scattering analysis, Fourier transform infrared spectroscopy, atomic absorption analysis, transmission electron microscopy and of X-ray diffraction analysis.ResultsAll AgNPcol showed spherical and positive zeta potential. The AgNPcol at a molar ratio of 1:6 showed better characteristics, smaller hydrodynamic diameter (64.34 ± 16.05) and polydispersity index (0.40 ± 0.05), and higher absorbance and silver reduction efficiency (0.645 mM), when compared with the particles prepared in other mixing ratios. Furthermore, these particles showed antimicrobial activity against both Staphylococcus aureus and Escherichia coli and no toxicity to the cells at the examined concentrations.ConclusionsThe resulted particles exhibited favorable characteristics, including the spherical shape, diameter between 64.34 nm and 81.76 nm, positive zeta potential, antibacterial activity, and non-toxicity to the tested cells (OSCC).Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-014-0036-6) contains supplementary material, which is available to authorized users.

Collagen-based silver nanoparticles: Study on cell viability, skin permeation, and swelling inhibition

“…4). This result corroborated with that obtained in previous studies [19], which demonstrated that AgNPcol is not cytotoxic.…”

“…The NaBH 4 solution was added later and the solution was homogenized for 10 min. Subsequently, the reaction mixture was centrifuged at 3600 rpm for 15 min (temperature 25°C) and finally separated from the supernatants of the final solution present in the container [19].…”

“…Alarcon et al [18] and Cardoso et al [19] used collagen type I, to stabilize the AgNPs starting from a silver nitrate solution. The authors conducted a study to test the biocompatibility and the antibacterial properties of AgNPs stabilized with collagen.…”

Collagen-Nanoparticles Composites for Wound Healing and Infection Control

“…It was reported that this material showed antimicrobial activity against both S. aureus and E. coli with no observed cellular toxicity at the tested concentrations (1:1, 1:6 and 1:15) [58]. In another study, an antiseptic collagen sponge was obtained by chemical reduction of Ag + in the presence of glucose and by plasma sputtering of Ag NPs onto the collagen sponge.…”

“…It was reported that this hybrid material retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels. In addition, it was observed that these materials present remarkable anti-infective properties against S. aureus, E. coli, S. epidermidis and P. aeruginosa at considerably lower concentrations than silver nitrate [57,58].…”