Collagen‐based matrices with axially oriented pores

Madaghiele, Marta; Sannino, Alessandro; Yannas, Ioannis V.; Spector, Myron

doi:10.1002/jbm.a.31517

Cited by 119 publications

(99 citation statements)

References 27 publications

(48 reference statements)

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“…A previous study reported that collagen concentration could influence the pore size and porosity, and that higher concentrations resulted in smaller pore sizes and lower porosity [24]. In our study, we observed the same results, both for the aligned collagen/silk scaffold and randomly aligned sponge collagen/silk scaffold, with the pore size and the porosity decreasing with increasing collagen concentration.…”

Section: Discussionsupporting

confidence: 88%

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Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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a b s t r a c tRotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, while commercially-available synthetic scaffolds are often inadequate at inducing tenogenic differentiation. The aim of this study is to develop an advanced 3D aligned collagen/silk scaffold (ACS) and investigate its efficacy in a rabbit massive rotator cuff tear model. ACS has similar 3D alignment of collagen fibers as natural tendon with superior mechanical characteristics. Based on ectopic transplantation studies, the optimal collagen concentration (10 mg/ml), pore diameter (108.43 ± 7.25 lm) and porosity (97.94 ± 0.08%) required for sustaining a stable macro-structure conducive for cellular infiltration was determined. Within in vitro culture, tendon stem/progenitor cells (TSPCs) displayed spindle-shaped morphology, and were well-aligned on ACS as early as 24 h. TSPCs formed intercellular contacts and deposited extracellular matrix after 7 days. With the in vivo rotator cuff repair model, the regenerative tendon of the ACS group displayed more conspicuous native microstructures with larger diameter collagen fibrils (48.72 ± 3.75 vs. 44.26 ± 5.03 nm) that had better alignment and mechanical properties (139.85 ± 49.36 vs. 99.09 ± 33.98 N) at 12 weeks post-implantation. In conclusion, these findings demonstrate the positive efficacy of the macroporous 3D aligned scaffold in facilitating rotator cuff tendon regeneration, and its practical applications for rotator cuff tendon tissue engineering. Statement of SignificanceMassive rotator cuff tear is one of the most common shoulder injuries, and poses a formidable clinical challenge to the orthopedic surgeon. Tissue engineering of tendon can potentially overcome the problem. However, more efficacious scaffolds with good biocompatibility, appropriate pore size, favorable inductivity and sufficient mechanical strength for repairing massive rotator cuff tendon injuries need to be developed. In this study, we developed a novel macroporous 3D aligned collagen/silk scaffold, and demonstrated that this novel scaffold enhanced the efficacy of rotator cuff tendon regeneration by inducing aligned supracellular structures similar to natural tendon, which in turn enhanced http://dx

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Section: Discussionsupporting

confidence: 88%

“…where V c is the volume occupied by the collagen, V t is the total volume of the scaffold, m is the mass of the scaffold, q is the density of anhydrous collagen, assumed to be 1.3 g/ml [24].…”

Section: Scaffold Fabricationmentioning

confidence: 99%

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Zheng¹,

Ran

Chen

et al. 2017

Acta Biomaterialia

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“…Type I microfibrillar collagen from bovine tendon (Collagen Matrix Inc., Oakland, NJ, USA) and chondroitin sulphate from shark cartilage were homogenised in 0.05 M acetic acid at a ratio of collagen to glycosaminoglycan of 11.25 : 1 (Madaghiele et al, 2008;Yannas et al, 1989). Anisotropic scaffolds were fabricated through lyophilisation, using a previously described directional solidification approach (Caliari and Harley, 2011;O'Brien et al, 2004).…”

Section: Fabrication Of Collagen-gag Scaffoldsmentioning

confidence: 99%

Cyclic tensile strain enhances human mesenchymal stem cell Smad 2/3 activation and tenogenic differentiation in anisotropic collagen-glycosaminoglycan scaffolds

Grier

Moy

Harley³

2017

eCM

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Orthopaedic injuries, particularly those involving ligaments and tendons, are some of the most commonly treated ailments in the United States and are associated with both high costs and poor outcomes. Regenerative medicine strategies for tendon injuries could be enhanced by three-dimensional biomaterials that can promote cell alignment and pro-tenogenic differentiation of patientderived MSCs. We have previously described a collagenglycosaminoglycan (CG) scaffold possessing aligned structural features able to promote bone marrow MSC differentiation towards a tenogenic lineage, in the absence of growth factor supplementation. We aimed to employ a bioreactor to enhance MSC tenogenic differentiation within the aligned CG scaffold via cyclic tensile strain (CTS), and further to evaluate the relative effects of strain cycle duration and extended application of repeated cycles of CTS on MSC response. Human MSCs were cultured in CG scaffolds for up to 6 d under static (unloaded) or cyclic tensile strain (1 Hz) for 10 min every 6 h. Time-dependent activation of ERK 1/2 and p38 mechanotransduction pathways was observed within each 6 h strain cycle. MSCs remained viable throughout the experiment and application of CTS robustly upregulated the expression of tendon-specific extracellular matrix proteins and phenotypic markers. Simultaneously, CTS promoted increased phosphorylation of Smad 2/3, suggesting a link between tensile stimulation and TGF-β family growth factor production. Together, we demonstrated the design, fabrication and validation of a high-throughput tensile stimulation bioreactor to increase MSC tenogenic differentiation in porous CG scaffolds.

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“…Furthermore, bovine as well as avian collagen show very low immunogenicity in vitro and can be safely used in vivo (Peng et al 2010). Therefore, collagen sponges and gels www.intechopen.com have been studied in vitro (Madaghiele et al 2008) and in vivo (Kroehne et al 2008). However, the rapid shrinkage of hydrogels in vitro as well as the low stability of collagen I are important disadvantages ) and limit its use in long-term experiments.…”

Section: Fig 1 Skeletal Muscle Precursor Cells (Myoblasts) In Vitromentioning

confidence: 99%

“…Therefore, several techniques for aligned scaffold architecture have been developed including selective laser sintering or three-dimensional printing reviewed by Karande et al (Karande et al 2004). Another method to gain spatially orientated pores in sponge-like matrices is unidirectional freeze-drying of materials like collagen (Madaghiele et al 2008) or silk fibroin ). Hydrogels are gradually frozen leading to controlled formation of ice crystals which result in controlled porosity after evaporation of the aqueous part of the hydrogel.…”

Section: Matricesmentioning

confidence: 99%