Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells

Yow, Soh-Zeom; Quek, Chai Hoon; Yim, Evelyn K.F.; Lim, Chwee Teck; Leong, Kam W.

doi:10.1016/j.biomaterials.2008.11.003

Cited by 58 publications

(56 citation statements)

References 36 publications

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“…However, current biomaterials used for scaffolding do not present a hierarchical structure as that found in natural materials and this could affect the cell behavior and differentiation. A rigorous understanding of collagen's scale and strain dependent stiffness may help in designing biomaterials with appropriate mechanical characteristics and thus addresses an immediate need for optimized matrix elasticity to foster differentiation and regeneration for regenerative medicine applications based on stem cell therapies such as cardiomyoplasty, muscular dystrophy, and neuroplasty [61][62][63]. To highlight the variation of Young's modulus and bending rigidity for a variety of biological and synthetic fibers we present a comparative analysis as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical Structure and Nanomechanics of Collagen Microfibrils from the Atomistic Scale Up

Gautieri

Vesentini²,

Redaelli³

et al. 2011

Nano Lett.

590

488

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Abstract:Collagen constitutes one third of the human proteome, providing mechanical stability, elasticity and strength to connective tissues. Collagen is also the dominating material in the extracellular matrix (ECM) and is thus crucial for cell differentiation, growth and pathology. However, fundamental questions remain with respect to the origin of the unique mechanical properties of collagenous tissues, and in particular its stiffness, extensibility and nonlinear mechanical response. By using x-ray diffraction data of a collagen fibril reported by Orgel et al.(Proceedings of the National Academy of Sciences USA, 2006) in combination with protein structure identification methods, here we present an experimentally validated model of the nanomechanics of a collagen microfibril that incorporates the full biochemical details of the amino acid sequence of the constituting molecules. We report the analysis of its mechanical properties under different levels of stress and solvent conditions, using a full-atomistic force field including explicit water solvent. Mechanical testing of hydrated collagen microfibrils yields a Young's modulus of ≈300 MPa at small and ≈1.2 GPa at larger deformation in excess of 10% strain, in excellent agreement with experimental data. Dehydrated, dry collagen microfibrils show a significantly increased Young's modulus of ≈1.8 to 2.25 GPa (or ≈6.75 times the modulus in the wet state) owing to a much tighter molecular packing, in good agreement with experimental measurements (where an increase of the modulus by ≈9 times was found). Our model demonstrates that the unique mechanical properties of collagen microfibrils can be explained based on their hierarchical structure, where deformation is mediated through mechanisms that operate at different hierarchical levels. Key mechanisms involve straightening of initially disordered and helically twisted molecules at small strains, followed by axial stretching of molecules, and eventual molecular uncoiling at extreme deformation. These mechanisms explain the striking difference of the modulus of collagen fibrils compared with single molecules, which is found in the range of 4.8±2 GPa or ≈10-20 times greater. These findings corroborate the notion that collagen tissue properties are highly scale dependent and nonlinear elastic, an issue that must be considered in the development of models that describe the interaction of cells with collagen in the extracellular matrix. A key impact the atomistic model of collagen microfibril mechanics reported here is that it enables the bottom-up elucidation of structure-property relationships in the broader class of collagen materials such as tendon or bone, including studies in the context of genetic disease where the incorporation of biochemical, genetic details in material models of connective tissue is essential.

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Section: Resultsmentioning

confidence: 99%

Hierarchical Structure and Nanomechanics of Collagen Microfibrils from the Atomistic Scale Up

Gautieri

Vesentini²,

Redaelli³

et al. 2011

Nano Lett.

590

488

View full text Add to dashboard Cite

show abstract

“…Additionally, poor mechanical properties necessitated post-fabrication treatment in the form of a sol-gel procedure. PEC fibers derived from methylated collagen and deprotonated terpolymer (2:1:1) of methyl methacrylate (MMA), 2-hydroxyethyl methacrylate (HEMA) and methacrylic acid (MAA) were prepared by Yow et al [7] to address the shortfalls of the earlier scaffolds. Integrin-rich collagen enhanced cellular interactions while terpolymer components afforded requisite physical properties.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously synthesized polyelectrolyte complexation (PEC) fibers from methylated collagen and synthetic terpolymer polyelectrolytes via interfacial polyelectrolyte complexation (IPC) for hMSC culture [7]. The ambient fiber drawing conditions avoid the denaturing of collagen and permit the formation of a bioactive PEC fiber.…”

Section: Introductionmentioning

confidence: 99%

“…Although numerous studies involving collagen-based scaffolds have been reported [7,[26][27][28], there is scant information on the application of collagen in conjunction with conducting materials in tissue engineering. This discrepancy could be ascribed to the lack of viable methods for the preparation of the required hybrids of collagen and conducting polymers.…”

Section: Introductionmentioning

confidence: 99%

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A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering

et al. 2011

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Fibers that can provide topographical, biochemical and electrical cues would be attractive for directing the differentiation of stem cells into electro-responsive cells such as neuronal or muscular cells. Here we report on the fabrication of polypyrrole-incorporated collagen-based fibers via interfacial polyelectrolyte complexation (IPC). The mean ultimate tensile strength of the fibers is 304.0 ± 61.0 MPa and the Young's Modulus is 10.4 ± 4.3 GPa. Human bone marrow-derived mesenchymal stem cells (hMSCs) are cultured on the fibers in a proliferating medium and stimulated with an external electrical pulse generator for 5 and 10 days. The effects of polypyrrole in the fiber system can be OPEN ACCESSPolymers 2011, 3 528 observed, with hMSCs adopting a neuronal-like morphology at day 10, and through the upregulation of neural markers, such as noggin, MAP2, neurofilament, β tubulin III and nestin. This study demonstrates the potential of this fiber system as an attractive 3D scaffold for tissue engineering, where collagen is present on the fiber surface for cellular adhesion, and polypyrrole is encapsulated within the fiber for enhanced electrical communication in cell-substrate and cell-cell interactions.

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“…Matthews et al demonstrated that collagen may be used to produce a nearly ideal scaffold for tissue engineering [59], whereas Yow et al showed that collagen can also be electrospun with other types of polymers, allowing efficient proliferation of human MSCs and maintenance of their multipotency for up to 7 days [60].…”

Section: Natural Polymersmentioning

confidence: 99%

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

et al. 2017

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Please cite this article as: Kitsara, M., Agbulut, O., Kontziampasis, D., Chen, Y., Menasché, P., Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering, Acta Biomaterialia (2016), doi: http://dx.doi.org/ 10. 1016/j.actbio.2016.11.014 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractCardiac cell therapy holds a real promise for improving heart function and especially of the chronically failing myocardium. Embedding cells into 3D biodegradable scaffolds may better preserve cell survival and enhance cell engraftment after transplantation, consequently improving cardiac cell therapy compared with direct intramyocardial injection of isolated cells.The primary objective of a scaffold used in tissue engineering is the recreation of the natural 3D environment most suitable for an adequate tissue growth. An important aspect of this commitment is to mimic the fibrillar structure of the extracellular matrix, which provides essential guidance for cell organization, survival, and function. Recent advances in nanotechnology have significantly improved our capacities to mimic the extracellular matrix. Among them, electrospinning is well known for being easy to process and cost effective. Consequently, it is becoming increasingly popular for biomedical applications and it is most definitely the cutting edge technique to make scaffolds that mimic the extracellular matrix for industrial applications.Here, the desirable physico-chemical properties of the electrospun scaffolds for cardiac therapy are described, and polymers are categorized to natural and synthetic. Moreover, the methods used for improving functionalities by providing cells with the necessary chemical cues and a more in vivo-like environment are reported.

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Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells

Cited by 58 publications

References 36 publications

Hierarchical Structure and Nanomechanics of Collagen Microfibrils from the Atomistic Scale Up

Hierarchical Structure and Nanomechanics of Collagen Microfibrils from the Atomistic Scale Up

A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering

Fibers for hearts: A critical review on electrospinning for cardiac tissue engineering

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