A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering

Yow, Soh-Zeom; Lim, Tze Han; Yim, Evelyn K.F.; Lim, Chwee Teck; Leong, Kam W.

doi:10.3390/polym3010527

Cited by 57 publications

(47 citation statements)

References 34 publications

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“…β‐tubulin III and nestin, which are cytoskeleton proteins, are the main marker of neural stem cells (NSCs) and neurons . Researchers found that neural gene markers including β‐tubulin and nestin had significant increases after the human bone marrow MSCs were cultivated on polypyrrole‐incorporated collagen fibrous scaffold and stimulated with 1.2‐V voltage, 200‐Hz frequency with 5‐ms pulse duration . In this study, the low‐level expression of GFAP (major intermediate filament proteins of mature astrocytes) was reported after electrical stimulus in both AC (EFs).…”

Section: Discussionmentioning

confidence: 75%

“…In the same study, researchers used an electrical stimulus with the 0.1‐Hz frequency which expressed the decreases of cell viability of this current . In another study, MSCs were stimulated on a PPy (Poly‐pyrrole) based substrate and it was reported that on the 5th day the cell proliferation increased, while on the 10th day it decreased compared to the nonstimulated group . In the present study, CJMSCs were stimulated on SF‐rGo conductive scaffold and the cell proliferation was examined on the third, fifth and seventh days after electrical stimulation (0.1 and 100 Hz).…”

Section: Discussionmentioning

confidence: 78%

“…75 In another study, MSCs were stimulated on a PPy (Poly-pyrrole) based substrate and it was reported that on the 5th day the cell proliferation increased, while on the 10th day it decreased compared to the nonstimulated group. 70 In the present study, CJMSCs were stimulated on SF-rGo conductive scaffold and the cell proliferation was examined on the third, fifth and seventh days after electrical stimulation (0.1 and 100 Hz). There were no differences in toxicity on the fifth day but on the seventh day, it decreased compared to 100 Hz.…”

Section: Discussionmentioning

confidence: 99%

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Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115 V/m, 100‐Hz frequency and current 2:115 v/m voltages, 0.1‐Hz frequency) were applied for 7 days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220 ± 20 nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of β‐tubulin (2.4‐fold P ≤ 0.026), MAP‐2 (1.48‐fold; P ≤ 0.03), and nestin (1.5‐fold; P ≤ 0.03) genes were higher in CJMSCs on conductive scaffold with 100‐Hz frequency compared to 0.1‐Hz frequency. Collectively, we proposed that SF‐rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

et al. 2019

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“…In spite of this, the number of studies devoted to fabricate bioactive platforms for tissue engineering based on the combination of collagen with CPs is, unfortunately, very scarce yet. [19][20][21][22][23] Thus, such studies are limited to the encapsulation of polypyrrole into collagen fibers, 19 the preparation of patterned platforms by inkjet printing polypyrrole and collagen lines on polyarate films, 20 the dispersion of polyaniline nanofibers in a collagen matrix, 21 and the preparation of CP-collagen composites using polypyrrole and polythiophene derivatives. [21][22][23] Poly (3,4-ethylenedioxythiophene), hereafter abbreviated PEDOT (Scheme 1), is one of the most important CPs due to its high electrical conductivity (up to 500 S/cm), excellent electrochemical properties and biocompatibility, and fast doping-dedoping processes.…”

Section: Introductionmentioning

confidence: 99%

Examining the compatibility of collagen and a polythiophene derivative for the preparation of bioactive platforms

et al. 2015

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Fundamental characteristics of bioactive platforms based on biocomposites of poly(3,4-ethylenedioxythiophene) (PEDOT) and collagen, named P(EDOT:CLG), have been examined using a experimental-computational approach. The protein affects both the morphology and electrochemical activity of PEDOT. Specifically, P(EDOT:CLG) show spherical-like nodules that has been attributed to the collagen rods aggregates organized in phases separated from that of PEDOT. This phase separation results in a reduction of the ability to exchange charge reversibly, even though collagen stabilizes the PEDOT matrix from electrochemical degradation.On the other hand, viability assays indicate that the bioactivity of P(EDOT:CLG) is significantly higher than that of PEDOT in terms of cellular adhesion and proliferation. Thus, the biocomposite promotes the formation of 3D biostructures formed by the superposition of cellular monolayers, mimiking the growing of biological tissues. In order to gain microscopic information about the formation of specific interactions between PEDOT and collagen molecules in the biocomposite, quantum mechanical calculations on complexes formed by their building blocks have been performed in different environments (i.e. vacuum, chloroform and aqueous solution). Results evidence the important role played by non-conventional C-H···O hydrogen bonds, which is consistent with previous findings on complexes involving DNA and dopamine.The environment affects considerably the binding energy, which decreases with increasing polarity of the environment. However, in all environment the repeat units of PEDOT form stronger interactions with L-hydroxyproline than with L-proline. On the other hand, intermolecular interaction patterns predicted using implicit and explicit solvation models present a very remarkable agreement and have been identified by visualizing the reduced electron density gradient.3

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“…The natural polyelectrolytes chitosan and alginate were used due to its high charge density and similarity to various ECM carbohydrates found in animal tissues. Yet methylated collagen and terpolymer 8,19 or chitin and alginate 20 may also be used for IPC fiber formation to varying effects.…”

mentioning

confidence: 99%

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Cutiongco

Teo

Yim

2015

JoVE

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Various scaffolds used in tissue engineering require a controlled biochemical environment to mimic the physiological cell niche. Interfacial polyelectrolyte complexation (IPC) fibers can be used for controlled delivery of various biological agents such as small molecule drugs, cells, proteins and growth factors. The simplicity of the methodology in making IPC fibers gives flexibility in its application for controlled biomolecule delivery. Here, we describe a method of incorporating IPC fibers into two different polymeric scaffolds, hydrophilic polysaccharide and hydrophobic polycaprolactone, to create a multi-component composite scaffold. We showed that IPC fibers can be easily embedded into these polymeric structures, enhancing the capability for sustained release and improved preservation of biomolecules. We also created a composite polymeric scaffold with topographical cues and sustained biochemical release that can have synergistic effects on cell behavior. Composite polymeric scaffolds with IPC fibers represent a novel and simple method of recreating the cellular niche. Video LinkThe video component of this article can be found at

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A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering

Cited by 57 publications

References 34 publications

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

Examining the compatibility of collagen and a polythiophene derivative for the preparation of bioactive platforms

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

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