Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy

Chevallay, B.; Herbage, D.

doi:10.1007/bf02344779

Cited by 334 publications

(200 citation statements)

References 76 publications

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“…Roeder et al showed, in agreement with the conclusions from Wood et al, that the fibril diameter decreases and the fibril length increases by increasing the pH, conjecturing that this change could explain the improvement in the mechanical properties [21,23]. While the mechanical properties increased as expected by increasing collagen concentration, collagen gels prepared at 2 mg/mL and different pH (6)(7)(8)(9) were stiffer at higher pH. The values of the linear modulus reported by these authors are lower than those presented in this work most probably for three reasons: a lower concentration of collagen in the gels, a lower pH during gel preparation and a lower testing rate.…”

Section: (Kpa)supporting

confidence: 68%

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Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

2010

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Collagen gels have been widely studied for applications in tissue engineering because of their biological implications. Considering their use as scaffolds for vascular tissue engineering, the main limitation has always been related to their low mechanical properties. During the process of in vitro self-assembly, which leads to collagen gelation, the size of the fibrils, their chemical interactions, as well as the resulting microstructure are regulated by three main experimental conditions: pH, ionic strength and temperature. In this work, these three parameters were modulated in order to increase the mechanical properties of collagen gels. The effects on the gelation process were assessed by turbidimetric and scanning electron microscopy analyses. Turbidity measurements showed that gelation was affected by all three factors and scanning electron images confirmed that major changes occurred at the microstructural level. Mechanical tests showed that the compressive and tensile moduli increased by four-and three-fold, respectively, compared to the control. Finally, viability tests confirmed that these gels are suitable as scaffolds for cellular adhesion and proliferation.

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Section: (Kpa)supporting

confidence: 68%

“…Scaffold-based vascular tissue engineering (VTE) proposes to combine cells and scaffolding systems in order to obtain vascular constructs [4]. Concerning the choice of the material for the scaffolds, collagen in particular is of significant interest because of its biological properties [6,7].…”

Section: Introductionmentioning

confidence: 99%

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

2010

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“…However, the material containing the cells must have sufficient flexibility for repetitive compressive stimulation. Collagen is a major component of extracellular matrix and is used as an in vitro organization model (Chevallay and Herbage 2000;Fox et al 2006;Stone et al 1992Stone et al , 1997Vernon and Gooden 2002). Contracted collagen gels (spheroids), in particular, are flexible under mechanical stress.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic acid secretion by synoviocytes alters under cyclic compressive load in contracted collagen gels

et al. 2013

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Knee osteoarthritis is a degenerative disease of diarthrodial joints. Biomechanical factors are considered as risk factors for the disease, the knee joint being normally subject to pressure. Some studies have examined the biomechanical environment of the knee joint in vitro. The aim of this study was to establish a culture model to mimic the knee joint environment. As a first step, synoviocytes induced contraction of threedimensional collagen gels. Next, contracted collagen gels containing synoviocytes underwent cyclical compression ranging from 0 to 40 kPa at a frequency of 1.0 Hz for 1.5, 3, 6 and 12 h using the FX-4000C TM Flexercell Ò Compression Plus TM System. RNA in collagen gels was extracted immediately after compression and mRNA expression levels of HAS genes were analyzed by quantitative RT-PCR. Culture medium was collected 48 h after compression and analyzed by agarose gel electrophoresis and cellulose acetate electrophoresis. Synoviocytes in contracted collagen gels were stimulated by cyclic compressive load. Long-term compressive stimulation led to the production of higher molecular weight hyaluronic acid, whereas, short-term, compressive stimulation increased the total amount of hyaluronic acid. Furthermore, mRNA expression levels of both HAS-1 and HAS-2 were significantly higher than without compression. Taken together, using this gel culture system, synoviocytes synthesized higher molecular weight hyaluronic acid and produced large quantities of hyaluronic acid through up-regulation of HAS gene expression. Therefore, the contracted collagen gel model will be a useful in vitro three-dimensional model of the knee joint.

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“…Such tunability or matrix remodeling has been implicated in diverse aspects of physiology including control of interstitial fluid pressure [2], aging [3], repair [4][5][6], fibrosis [7,8] and tumorigenesis [9]. Not surprisingly, matrix remodeling also is an important consideration for tissue engineering [10][11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Fibroblast mechanics in 3D collagen matrices☆

Rhee

Grinnell²

2007

Advanced Drug Delivery Reviews

163

115

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Connective tissues provide mechanical support and frameworks for the other tissues of the body. Type 1 collagen is the major protein component of ordinary connective tissue, and fibroblasts are the cell type primarily responsible for its biosynthesis and remodeling. Research on fibroblasts interacting with collagen matrices explores all four quadrants of cell mechanics: pro-migratory vs. pro-contractile growth factor environments on one axis; high tension vs. low tension cell-matrix interactions on the other. The dendritic fibroblast -probably equivalent to the resting tissue fibroblast -can be observed only in the low tension quadrant and generally has not been appreciated from research on cells incubated with planar culture surfaces. Fibroblasts in the low tension quadrant require microtubules for formation of dendritic extensions, whereas fibroblasts in the high tension quadrant require microtubules for polarization but not for spreading. Ruffling of dendritic extensions rather than their overall protrusion or retraction provides the mechanism for remodeling of floating collagen matrices, and floating matrix remodeling likely reflects a model of tissue mechanical homeostasis.

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Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy

Cited by 334 publications

References 76 publications

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

Hyaluronic acid secretion by synoviocytes alters under cyclic compressive load in contracted collagen gels

Fibroblast mechanics in 3D collagen matrices☆

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