Collaborative validation study of the in vivo micronucleus test using mouse colonic epithelial cells

Ohyama, Wakako; Gonda, Maki; Miyajima, Hirofumi; Kono, Koji; Noguchi, Tadashi; Yoshida, Junichi; Hatakeyama, Sigeki; Watabe, Etsuko; Ueno, Yuka; Hayashi, Makoto; Tokumitsu, Takashi

doi:10.1016/s1383-5718(02)00061-x

Cited by 31 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Short-term tests such as the mouse bone marrow micronucleus assay as well as assessment of ROS-induced lipid peroxidation and the status of antioxidants are widely used in the detection and evaluation not only of environmental carcinogens, but also of presumptive antimutagens and anticarcinogens (6,7). The present study was designed to evaluate the effect of oral pretreatment with tomato and garlic alone and in combination on DMBA-induced genotoxicity and oxidative stress.…”

Section: Bhuvaneswari Et Almentioning

confidence: 99%

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice

Bhuvaneswari

Velmurugan

Abraham

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

Chemoprotection by dietary agents is a promising strategy for cancer prevention. The aim of the present study was to evaluate the combined effect of tomato and garlic against 7,12-dimethylbenz-[a]anthracene (DMBA)-induced genetic damage and oxidative stress in 12-14-week-old male Swiss albino mice. The animals were randomized into experimental and control groups and divided into eight groups of five animals each. Group 1 animals were injected intraperitoneally with 35 mg/kg body weight DMBA suspended in peanut oil as a single dose. Groups 2-4 animals received tomato (500 mg/kg body weight), garlic (125 mg/kg body weight) and a combination of tomato and garlic for 5 days by gavage, respectively, followed by DMBA 1.5 h after the final feeding. The doses of tomato and garlic correspond to the average human daily consumption. Animals in groups 5, 6 and 7 received tomato alone, garlic alone and tomato + garlic combination, respectively, for 5 days. Group 8 animals received the same volume of water and served as control. The incidence of bone marrow micronuclei and the extent of lipid peroxidation and the concentrations of antioxidants glutathione, glutathione peroxidase and glutathione-S-transferase were measured in the liver, 48 h after DMBA exposure. Increased frequency of micronuclei and enhanced lipid peroxidation accompanied by compromised antioxidant defenses were observed in DMBA-treated animals. Although pretreatment with tomato or garlic significantly reduced the frequency of DMBAinduced bone marrow micronuclei, the combination of tomato and garlic exhibited more profound effect in inhibiting DMBA-induced genotoxicity and oxidative stress. We suggest that a broad spectrum of antimutagenic and anticlastogenic effects can be achieved through an effective combination of functional foods such as tomato and garlic.

show abstract

Section: Bhuvaneswari Et Almentioning

confidence: 99%

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice

Bhuvaneswari

Velmurugan

Abraham

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…Although these tissues are not targeted in the routine MN assay, they have already been studied for MN induction by CSGMT/MMS/JEMS, and the results have been published previously [4][5][6][7][8]. In this new collaborative study, we evaluated the revised version of the MN assay system targeting the liver and GI tract after repeated-dose treatments without any other treatment for the animals; therefore, the genotoxic endpoints can be incorporated into repeated-dose general toxicological studies.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the repeated-dose liver and gastrointestinal tract micronucleus assays with 22 chemicals using young adult rats: Summary of the collaborative study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/The Japanese Environmental Mutagen Society (JEMS) – Mammalian Mutagenicity Study Group (MMS)

Hamada

Ohyama

Takashima³

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

Self Cite

View full text Add to dashboard Cite

The repeated-dose liver micronucleus (RDLMN) assay using young adult rats has the potential to detect hepatocarcinogens. We conducted a collaborative study to assess the performance of this assay and to evaluate the possibility of integrating it into general toxicological studies. Twenty-four testing laboratories belonging to the Mammalian Mutagenicity Study Group, a subgroup of the Japanese Environmental Mutagen Society, participated in this trial. Twenty-two model chemicals, including some hepatocarcinogens, were tested in 14- and/or 28-day RDLMN assays. As a result, 14 out of the 16 hepatocarcinogens were positive, including 9 genotoxic hepatocarcinogens, which were reported negative in the bone marrow/peripheral blood micronucleus (MN) assay by a single treatment. These outcomes show the high sensitivity of the RDLMN assay to hepatocarcinogens. Regarding the specificity, 4 out of the 6 non-liver targeted genotoxic carcinogens gave negative responses. This shows the high organ specificity of the RDLMN assay. In addition to the RDLMN assay, we simultaneously conducted gastrointestinal tract MN assays using 6 of the above carcinogens as an optional trial of the collaborative study. The MN assay using the glandular stomach, which is the first contact site of the test chemical when administered by oral gavage, was able to detect chromosomal aberrations with 3 test chemicals including a stomach-targeted carcinogen. The treatment regime was the 14- and/or 28-day repeated-dose, and the regime is sufficiently promising to incorporate these methods into repeated-dose toxicological studies. The outcomes of our collaborative study indicated that the new techniques to detect chromosomal aberrations in vivo in several tissues worked successfully.

show abstract

“…Short-term tests such as the mouse bone marrow micronucleus assay, chromosomal aberration analysis, as well as the assessment of antioxidant status and reactive oxygen species (ROS)-induced lipid peroxidation (LPO), are widely used in the detection and evaluation not only of environmental carcinogens but also of presumptive antimutagens and anticarcinogens [Locigno and Castronovo, 2001;Ohyama et al, 2002]. Therefore, the present investigation was undertaken to evaluate the activity of Mentha piperita leaf extract on benzo[a]pyrene (BP)-induced lung carcinogenicity, genotoxicity, and oxidative stress in Swiss albino mice.…”

Section: Introductionmentioning

confidence: 99%

Modulatory effects of Mentha piperita on lung tumor incidence, genotoxicity, and oxidative stress in benzo[a]pyrene‐treated Swiss albino mice

Samarth

Panwar

Kumar

2005

Environ and Mol Mutagen

View full text Add to dashboard Cite

Mentha piperita or peppermint is currently used for alleviating nausea, flatulence, and vomiting. In the present investigation, we evaluated the chemopreventive, antigenotoxic, and antioxidative effects of an aqueous extract of Mentha piperita leaves. One-day-old Swiss albino mice were treated with a single subcutaneous injection of 0.5 mg benzo[a]pyrene (BP) and then given either water or a Mentha extract (ME; 1 g/kg body weight) by gavage starting at 3 weeks of age (weaning). The mice were killed at 9 weeks of age and tested for lung tumor incidence (chemoprevention); bone marrow micronucleus and chromosome aberration frequency (antigenotoxicity); and levels of liver and lung sulfhydral groups, superoxide dismutase (SOD) and catalase (CAT) activity, and lipid peroxidation (LPO) (antioxidative properties). The ME treatment resulted in a significant reduction in the number of lung adenomas from an incidence of 67.92% in animals given only BP to 26.31%, an inhibition of 61.26%. Tumor multiplicity was 1.22 in the BP-alone group and 1.15 in the BP + ME group. In addition, compared with the animals in the BP-alone group, ME reduced the frequency of chromosomal aberrations and micronuclei in bone marrow cells and decreased the levels of LPO and increased reduced glutathione content, and SOD and CAT activities in liver as well as lung. The results of this study indicate that ME is chemopreventive and antigenotoxic when given subsequent to an initiating dose of BP in newborn Swiss albino mice. The chemopreventive action and antigenotoxic effects observed in the present study may be due to the antioxidative properties of ME.

show abstract

Collaborative validation study of the in vivo micronucleus test using mouse colonic epithelial cells

Cited by 31 publications

References 13 publications

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice

Tomato and garlic by gavage modulate 7,12-dimethylbenz[a]anthracene-induced genotoxicity and oxidative stress in mice

Modulatory effects of Mentha piperita on lung tumor incidence, genotoxicity, and oxidative stress in benzo[a]pyrene‐treated Swiss albino mice

Contact Info

Product

Resources

About