Collaborative Study on a Method for Detection of Aflatoxin B1 in Green Coffee Beans

Levi, Colette P

doi:10.1093/jaoac/52.6.1300

Cited by 1 publication

(3 citation statements)

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“…The proposed method is a one-step minicolumn cleanup procedure that uses the unique property of Florisil to adsorb aflatoxins and a limited number of other compounds from highly polar solvents such as MeOH and H 2 O. Such a technique was overlooked in previous studies (4,(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) that considered Florisil to be a traditional chromatographic adsorbent; conventional elution patterns used large quantities of toxic organic solvents.…”

Section: Resultsmentioning

confidence: 99%

“…Aflatoxins can be eluted from the Florisil column only with acetone (18) and acetone/H 2 O mixtures (22). Optimization of the elution solvent was required on the basis of the fact that aflatoxins can be eluted from the Florisil column only with large volumes of acetone (18), acetone/MeOH (27,29), and acetone/ H 2 O mixtures (4,22,24,25). The most reasonable proportions of acetone-based solvents were tested.…”

Section: Resultsmentioning

confidence: 99%

“…A Florisil minicolumn for the adsorption, detection, and estimation of aflatoxin concentrations was introduced by Velasco (19) in 1972; a modified version of the minicolumn was accepted as an official AOAC method (20). Since then, several cleanup methods using Florisil were offered and tested (4,(21)(22)(23)(24)(25)(26)(27)(28)(29); however, all of them had significant disadvantages. The major drawback was that the methods were time-consuming and used significant quantities of toxic organic solvents both for extraction and for column cleanup.…”

Section: Introductionmentioning

confidence: 99%

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Simple, Rapid, and Inexpensive Cleanup Method for Quantitation of Aflatoxins in Important Agricultural Products by HPLC

Соболев

2007

J. Agric. Food Chem.

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A chemical cleanup procedure for low-level quantitative determination of aflatoxins in major economically important agricultural commodities using HPLC has been developed. Aflatoxins were extracted from a ground sample with MeOH/H2O (80:20, v/v), and after a cleanup step on a minicolumn packed with Florisil, aflatoxins were quantified by HPLC equipped with a C18 column, a photochemical reactor, and a fluorescence detector. Water/MeOH (63:37, v/v) served as the mobile phase. Recoveries of aflatoxins B1, B2, G1, and G2 from peanuts spiked at 5, 1.7, 5, and 1.7 ng/g were 89.5+/-2.2, 94.7+/-2.5, 90.4+/-1.0, and 98.2+/-1.1, respectively (mean+/-SD, %, n=3). Similar recoveries, precision, and accuracy were achieved for corn, brown and white rice, cottonseed, almonds, Brazil nuts, pistachios, walnuts, and hazelnuts. The quantitation limits for aflatoxins in peanuts were 50 pg/g for aflatoxin B1 and 17 pg/g for aflatoxin B2. The minimal cost of the minicolumn allows for substantial savings compared with available commercial aflatoxin cleanup devices.

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