Cold stress-induced lipid peroxidation and non-enzymatic antioxidant defense in tissues of the common Indian toad, Bufo melanostictus

Sahoo, Deba Das; Kara, T.C.

doi:10.2298/abs1404303s

Cited by 13 publications

(6 citation statements)

References 42 publications

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“…In this study, the activities of SOD and GPx in liver were significantly elevated with an increase of mitochondrial ROS generation, indicating that these enzymes are involved in eliminating the extra ROS generation induced by cold and heat stress, which is consistent with the results in other poikilotherms such as the pufferfish Takifugu obscurus (Cheng et al, 2015(Cheng et al, , 2017, the predatory mite Neoseiulus cucumeris (Zhang et al, 2014) and the whiteleg shrimp Litopenaeus vannamei (Xu et al, 2018;Zhou et al, 2010). Remarkably, the activity of GPx showed a quicker response to cold stress than heat stress, which could be due to the fact that GPx is considered to be a key antioxidant enzyme to reduce lipid hydroperoxide under cold stress (Dawson and Storey, 2017;Feidantsis et al, 2013;Sahoo and Kara, 2014). Moreover, the activities of SOD and GPx subsequently returned to baseline levels, whereas the CAT activity significantly increased after 24 h under cold stress.…”

Section: Discussionsupporting

confidence: 89%

“…Owing to their special sensitivity to temperature changes, amphibians could serve as model organisms to investigate the effects of cold or heat stress. Previous studies on the effects of cold stress on amphibians have shown the upregulation of freezeresponsive molecules (McNally et al, 2002;Storey and Storey, 2017;Wu et al, 2008), regulation of redox balance Storey, 2016, 2017;Sahoo and Kara, 2014), reduction of lymphocyte (Greenspan et al, 2017;Maniero and Carey, 1997) and synthesis of the cryoprotectants urea and glucose (Costanzo and Lee, 2008;Costanzo et al, 2013Costanzo et al, , 2015Dieni et al, 2012), whereas heat stress upregulates the heat shock response (Heikkila, 2010;Young et al, 2009), antioxidant defence system and the susceptibility of amphibians to harmful substances Gaitanaki et al, 2007). Furthermore, thermal stresses also can effectively induce histological alterations in the liver of other ectotherms, such as fat droplet accumulation (Liu et al, 2016), mitochondrial swelling (Xu et al, 2018), hepatocyte degeneration and necrosis (Ates et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Liu

et al. 2018

Journal of Experimental Biology

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Ambient temperature-associated stress can affect normal physiological functions in ectotherms. To assess the effects of cold or heat stress on amphibians, giant spiny frogs () were acclimated at 22°C followed by exposure to 5°C or 30°C for 0, 3, 6, 12, 24 and 48 h, respectively. Histological alterations, apoptotic index, generation of mitochondrial reactive oxygen species (ROS), antioxidant activity indices and stress-response gene expression in frog livers were subsequently determined. Results showed that many fat droplets appeared after 12 h of heat stress and the percentage of melanomacrophage centres significantly changed after 48 h at both stress conditions. Furthermore, the mitochondrial ROS levels were elevated in a time-dependent manner up to 6 h and 12 h in the cold and heat stress groups, respectively. The activities of superoxide dismutase, glutathione peroxidase and catalase were successively increased with increasing periods of cold or heat exposure, and their gene expression levels showed similar changes in both stress conditions. Most tested heat shock protein (HSP) genes were sensitive to temperature exposure, and the expression profiles of most apoptosis-related genes was significantly upregulated at 3 and 48 h under cold and heat stress, respectively. Apoptotic index at 48 h under cold stress was significantly higher than that under heat stress. Notably, lipid droplets, , and might be suitable bioindicators of heat stress. The results of these alterations at physiological, biochemical and molecular levels might contribute to a better understanding of the stress response of, and perhaps amphibians more generally, under thermal stress.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Liu

et al. 2018

Journal of Experimental Biology

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“…Unlike TAC in those 3 tissues, renal TAC was not affected by cold exposure. This may be due to the detention of antioxidants produced in kidney, such as ascorbic acid or uric acid (Sahoo & Kara 2014;Chen et al 2015). Rewarming also decreased TAC in the brain, liver and spleen (Fig.…”

Section: Discussionmentioning

confidence: 91%

“…Kammer et al (2011) report that cold exposure increased antioxidant enzyme activities and the carbonyl protein level, which is a marker of oxidative damage, in the liver of three spine sticklebacks, Gasterosteus aculeatus L., 1758. Lipid peroxidation and non-enzymatic antioxidant defense increased in cold-stressed toad, Bufo melanostictus Schneider, 1799 (Sahoo & Kara 2014). These two reports indicate a potential production of excess ROS caused by cold stress.…”

Section: Introductionmentioning

confidence: 85%

Effects of acute cold exposure on oxidative balance and total antioxidant capacity in juvenile Chinese soft‐shelled turtle, Pelodiscus sinensis

Zhang

Niu

Jia

et al. 2017

Integrative Zoology

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Acute cold exposure may disturb the physiological homeostasis of the body in ectotherms. To date, there has been no information on the effects of cold exposure on homeostasis of reactive oxygen species (ROS) or antioxidant defense response in the Chinese soft-shelled turtle, Pelodiscus sinensis. In this study, P. sinensis juveniles were acclimated at 28 °C, transferred to 8 °C as cold exposure for 12 h, then moved back to 28 °C rewarming for 24 h. We measured the ROS level and total antioxidant capacity (TAC) in the brain, liver, kidney and spleen at 2 and 12 h cold exposure, and at the end of the rewarming period. Malonaldehyde (MDA) and carbonyl protein were used as markers of oxidative damage. Turtles being maintained simultaneously at 28 °C were used as the control group. Cold exposure did not disturb the ROS balance in all 4 tissues, while rewarming raised the ROS level in the brain and kidney of P. sinensis. Cold exposure and rewarming decreased the TAC in the brain, liver and spleen but did not change the TAC in the kidney. MDA and carbonyl protein levels did not increase during the treatment, indicating no oxidative damage in all 4 tissues of P. sinensis. Our results indicated that extreme cold exposure did not impact the inner oxidative balance of P. sinensis, but more ROS was produced during rewarming. P. sinensis showed good tolerance to the harsh temperature change through effective protection of its antioxidant defense system to oxidative damage. This study provides basic data on the stress biology of P. sinensis.

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“…ROS-mediated oxidation of amino acid residues, inparticular proline, arginine and lysine of protein in animal cells to their carbonyl derivatives, increases exponentially with exposure to stressed conditions [2,3]. Similarly, oxidative damage of lipids, resulting in a wide variety of lipid peroxidation products such as malondialdehyde, hexanol, and 4-hydroxyalkenals, has been reported to increase during stressed conditions in insects, rotifers, fishes, amphibians, reptiles and mammals [4][5][6]. Thus, lipid peroxidation and protein carbonylation are considered to be oxidative stress markers in a variety of living organisms [7][8].…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress markers and antioxidant defense in hibernating common Asian toads, Duttaphrynus melanostictus

Sahoo¹,

Patnaik

2020

ARCH BIOL SCI BELGRA

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To assess the oxidative assaults and antioxidant defense, oxidative stress markers, including lipid peroxidation level, protein carbonylation level, GSSG/GSH ratio and nonenzymatic antioxidants such as total glutathione, ascorbic acid and uric acid, in liver and brain tissues of hibernating common Asian toads, Duttaphrynus melanostictus, were compared with toads during active periods. Oxidative stress was found in both liver and brain tissues of hibernating common Asian toads in spite of depressed metabolism and low oxygen consumption. Significantly higher lipid peroxidation, protein carbonylation and an increased GSSG/GSH ratio were found in liver and brain tissues of hibernating toads, indicating oxidative stress. To counteract the stress, ascorbic acid was increased significantly in the liver and brain tissues of hibernating individuals in comparison to individuals during active periods. The uric acid level decreased in both the liver and brain tissues of hibernating toads, which may be due to its decreased rate of synthesis because of low xanthine oxidase activity at low body temperature and hypometabolism. The common Asian toad faced oxidative stress during hibernation, which was counteracted by augmented nonenzymatic antioxidant defense.

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Cold stress-induced lipid peroxidation and non-enzymatic antioxidant defense in tissues of the common Indian toad, Bufo melanostictus

Cited by 13 publications

References 42 publications

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Effects of both cold and heat stresses on the liver of giant spiny frog Quasipaa spinosa: stress response and histological changes

Effects of acute cold exposure on oxidative balance and total antioxidant capacity in juvenile Chinese soft‐shelled turtle, Pelodiscus sinensis

Oxidative stress markers and antioxidant defense in hibernating common Asian toads, Duttaphrynus melanostictus

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