Oxidative stress markers and antioxidant defense in hibernating common Asian toads, Duttaphrynus melanostictus

Abstract

To assess the oxidative assaults and antioxidant defense, oxidative stress markers, including lipid peroxidation level, protein carbonylation level, GSSG/GSH ratio and nonenzymatic antioxidants such as total glutathione, ascorbic acid and uric acid, in liver and brain tissues of hibernating common Asian toads, Duttaphrynus melanostictus, were compared with toads during active periods. Oxidative stress was found in both liver and brain tissues of hibernating common Asian toads in spite of depressed metabolism and low oxygen consumption. Significantly higher lipid peroxidation, protein carbonylation and an increased GSSG/GSH ratio were found in liver and brain tissues of hibernating toads, indicating oxidative stress. To counteract the stress, ascorbic acid was increased significantly in the liver and brain tissues of hibernating individuals in comparison to individuals during active periods. The uric acid level decreased in both the liver and brain tissues of hibernating toads, which may be due to its decreased rate of synthesis because of low xanthine oxidase activity at low body temperature and hypometabolism. The common Asian toad faced oxidative stress during hibernation, which was counteracted by augmented nonenzymatic antioxidant defense.

Variations in oxidative stress and antioxidant defense level during different phases of hibernation in common Asian toad,Duttaphrynus melanostictus

“…In response to increased ROS generation and oxidative stress, adequate antioxidant defense comprising both antioxidant enzymes and non-enzymatic antioxidants are required for the maintenance of redox homeostasis during different phases of hibernation. In our previous work ( Sahoo and Patnaik, 2020 ), elevated nonenzymatic antioxidant status during hibernation were reported in the common Asian toad, Duttaphrynus melanostictus. Increased oxidative stress and antioxidant activities have also been reported during hibernation in endothermic mammals ( Okamoto et al, 2006 ; Yin et al, 2016 ; Wei et al, 2018 ).…”