Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

Muzi, Carlo; Camoni, Lorenzo; Visconti, Sabina; Aducci, Patrizia

doi:10.1016/j.plaphy.2016.07.027

Cited by 56 publications

(34 citation statements)

References 57 publications

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“…4). This suggested that ATP hydrolysis in RIL82 plants were adversely inhibited by cold stress, and thus the lower GSH synthesis because of the higher unavailable ATP (Puhakainen et al 1999;Mendoza et al 2000;Deng et al 2015;Muzi et al 2016). This hypothesis was confirmed by the present results that the PARP inhibitor (3-ab) only enhanced the cold tolerance and GSH content in ZZ39 plants under cold stress ( Fig.…”

Section: The Role Of Atp Hydrolysis In Gsh Synthesis In Rice Plants Usupporting

confidence: 84%

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Jiang

et al. 2020

Rice

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Background: Glutathione (GSH) is important for plants to resist abiotic stress, and a large amount of energy is required in the process. However, it is not clear how the energy status affects the accumulation of GSH in plants under cold stress. Results: Two rice pure lines, Zhongzao39 (ZZ39) and its recombinant inbred line 82 (RIL82) were subjected to cold stress for 48 h. Under cold stress, RIL82 suffered more damages than ZZ39 plants, in which higher increases in APX activity and GSH content were showed in the latter than the former compared with their respective controls. This indicated that GSH was mainly responsible for the different cold tolerance between these two rice plants. Interestingly, under cold stress, greater increases in contents of carbohydrate, NAD(H), NADP(H) and ATP as well as the expression levels of GSH1 and GSH2 were showed in RIL82 than ZZ39 plants. In contrast, ATPase content in RIL82 plants was adversely inhibited by cold stress while it increased significantly in ZZ39 plants. This indicated that cold stress reduced the accumulation of GSH in RIL82 plants mainly due to the inhibition on ATP hydrolysis rather than energy deficit. Conclusion: We inferred that the energy status determined by ATP hydrolysis involved in regulating the cold tolerance of plants by controlling GSH synthesis.

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Section: The Role Of Atp Hydrolysis In Gsh Synthesis In Rice Plants Usupporting

confidence: 84%

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Jiang

et al. 2020

Rice

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“…() as well as Muzi et al . () for examples) and not at the restrictive, low temperatures that we note can affect ATP synthase function.…”

Section: Discussionmentioning

confidence: 79%

“…Thus, when plants are returned to higher, more moderate temperatures, ATP synthases or hydrolysis is enhanced. This phenomenon may explain why some studies report significant increases in ATP synthase or ATP lyase activity in the cold, as such experiments only involved measurements of the enzyme at 25°C or above (see Schulze et al (2012) as well as Muzi et al (2016) for examples) and not at the restrictive, low temperatures that we note can affect ATP synthase function.…”

Section: Researchmentioning

confidence: 96%

Cold sensitivity of mitochondrial ATP synthase restricts oxidative phosphorylation in Arabidopsis thaliana

2018

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Summary The combined action of the electron transport chain (ETC) and ATP synthase is essential in determining energy efficiency in plants, and so is important for cellular biosynthesis, growth and development. Owing to the sessile nature of plants, mitochondria must operate over a wide temperature range in the environment, necessitating a broad temperature tolerance of their biochemical reactions. We investigated the temperature response of mitochondrial respiratory processes in isolated mitochondria and intact plants of Arabidopsis thaliana and considered the effect of instantaneous responses to temperature and acclimation responses to low temperatures. We show that at 4°C the plant mitochondrial ATP synthase is differentially inhibited compared with other elements of the respiratory pathway, leading to decreased ADP : oxygen ratios and a limitation to the rate of ATP synthesis. This effect persists in vivo and cannot be overcome by cold‐temperature acclimation of plants. This mechanism adds a new element to the respiratory acclimation model and provides a direct means of temperature perception by plant mitochondria. This also provides an alternative explanation for non‐phosphorylating ETC bypass mechanisms, like the alternative oxidase to maintain respiratory rates, albeit at lower ATP synthesis efficiency, in response to the sensitivity of ATP synthase to the prevailing temperature.

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“…Moreover, PLD-derived PA may activate proton-pumps, like vacuolar H + -ATPase and H + -PPase, which confer a proton gradient for the Na + /H + exchange to enhance salt tolerance (Zhang et al, 2006; Shen et al, 2011). A new study also suggested that there was a functional correlation between the activities of PLD and the H + -ATPase mediated by PA release (Muzi et al, 2016). In this study, we monitor the expression levels of genes related to ion exchange, including NtNHX1 , NtNKT1 and NtHAK1 , and genes related to H + -ATPase, such as NtNHA1 and NtVAG1 , under short-term NaCl stress.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Cucumber Phospholipase D alpha Gene (CsPLDα) in Tobacco Enhanced Salinity Stress Tolerance by Regulating Na+–K+ Balance and Lipid Peroxidation

Huang

et al. 2017

Front. Plant Sci.

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Plant phospholipase D (PLD), which can hydrolyze membrane phospholipids to produce phosphatidic acid (PA), a secondary signaling molecule, has been proposed to function in diverse plant stress responses. In this research, we characterized the roles of the cucumber phospholipase D alpha gene (PLDα, GenBank accession number EF363796) in growth and tolerance to short- and long-term salt stress in transgenic tobacco (Nicotiana tabacum). Fresh and dry weights of roots, PLD activity and content, mitogen activated protein kinase (MAPK) gene expression, Na+–K+ homeostasis, expression of genes encoding ion exchange, reactive oxygen species (ROS) metabolism and osmotic adjustment substances were investigated in wild type (WT) and CsPLDα-overexpression tobacco lines grown under short- and long-term high salt (250 mM) stress. Under short-term stress (5 h), in both overexpression lines, the PA content, and the expression levels of MAPK and several genes related to ion exchange (NtNHX1, NtNKT1, NtHAK1, NtNHA1, NtVAG1), were promoted by high PLD activity. Meanwhile, the Na+/K+ ratio decreased. Under long-term stress (16 days), ROS scavenging systems (superoxide dismutase, peroxidase, catalase, ascorbate peroxidase activities) in leaves of transgenic lines were more active than those in WT plants. Meanwhile, the contents of proline, soluble sugar, and soluble protein significantly increased. In contrast, the contents of O2•− and H2O2, the electrolytic leakage and the accumulation of malondialdehyde in leaves significantly decreased. The root fresh and dry weights of the overexpression lines increased significantly. Na+–K+ homeostasis had the same trend as under the short-term treatment. These findings suggested that CsPLDα-produced PA can activate the downstream signals’ adaptive response to alleviate the damage of salt stress, and the main strategies for adaptation to salt stress are the accumulation of osmoprotective compounds, maintaining Na+–K+ homeostasis and the scavenging of ROS, which function in the osmotic balancing and structural stabilization of membranes.

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Cold stress affects H + -ATPase and phospholipase D activity in Arabidopsis

Cited by 56 publications

References 57 publications

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Cold sensitivity of mitochondrial ATP synthase restricts oxidative phosphorylation in Arabidopsis thaliana

Overexpression of Cucumber Phospholipase D alpha Gene (CsPLDα) in Tobacco Enhanced Salinity Stress Tolerance by Regulating Na+–K+ Balance and Lipid Peroxidation

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