Cold Storage of Milt from Four Species of Flatfish

Jeong, Chang Yun; Jin, Chang Young; Kyu, Lim Han; Kwan, Lee Jong; Je, Park Young

doi:10.5657/fas.2002.5.1.064

Cited by 2 publications

(3 citation statements)

References 13 publications

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“…In this study, Stein's solution, was the most effective extender for the cold storage of spotted halibut sperm. Indeed, similar findings have been reported for olive flounder (Paralichthys olivaceus) [5], brown sole (Pleuronectes herzensteini) [6], marbled sole (Limanda yokohamae), and starry flounder (Platichthyes stellatus) [7]. In this study, MFRS, 300 mM sucrose, and 300 mM glucose reduced sperm quality.…”

Section: Discussionsupporting

confidence: 90%

“…In practice, cold storage of sperm promotes egg production in hatcheries because it is a low-cost and easy means of overcoming the insufficient number of males. The effect of short-term storage of fish sperm has been evaluated in marine fish species such as olive flounder (Paralichthys olivaceus) [5], marbled sole (Limanda yokohamae), brown sole (Limanda herzensteini) [6], starry flounder (Platichthys stellatus) [7], yellow croaker (Larimichthys polyactis) [8], and giant grouper (Epinephelus lanceolatus) [9]. However, to our knowledge, no study has evaluated the influence of short-term preservation of spotted halibut sperm on sperm function.…”

Section: Introductionmentioning

confidence: 99%

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Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of >43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Intermediate-Term Storage of Spotted Halibut (Verasper variegatus) Sperm: Effects of Storage Methods, Extenders Supplemented with Antibiotics and Antioxidants on Sperm Quality

et al. 2023

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show abstract

“…Both cold storage and cryopreservation can be used for milt preservation in aquaculture (Migaud et al, 2013). Cold storage requires storing milt diluted in extenders at low temperatures (typically 4 °C, (Gallego and Asturiano., 2019)) to reduce spermatozoa metabolism allowing them to be stored from 4 days in turbot, Scophthalmus maximus (Chereguini et al, 1997) to 56 days in cod, Gadus morhua (Degraaf and Berlinsky, 2004) without significant changes in milt quality (Chang, 2002). For longer term storage, between spawning seasons (Scott and Baynes, 1980) or for creating genetic storage banks (Gausen, 1993), cryopreservation is the only effective method, keeping milt diluted in a cryoprotectant solution at ultralow temperatures between -79 and -196 °C in liquid nitrogen.…”

Section: Introductionmentioning

confidence: 99%