Cold‐Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge

Abstract

Antioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin-Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of d-tocopherol (0.87), squalene (0.67) and color CIE a* (-0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.

The Influence of Drying Process Conditions on the Physical Properties, Bioactive Compounds and Stability of Encapsulated Pumpkin Seed Oil

“…In the presented experiment, the content of squalene in the control sample reached 1192 mg/100 g of oil, which is a high value compared to literature data. According to Gorjanović et al (2011), squalene content in oils produced from seeds of C. pepo hulless pumpkin ranged from 549 mg/100 g of oil (cultivar BSB^-Serbia) to 788 mg/100 g of oil (cultivar BGleisdorfer Diamant^-Austria). The analysis of results obtained in our study enables concluding that squalene is a compound characterized by high lability.…”

“…The stability of control pumpkin seed oil expressed as the oxidation induction time was 6.1 h. Some discrepancies occur in literature data regarding the oxidative stability of pumpkin seed oil. For example, Gorjanović et al (2011) found that the induction time of oil obtained from seeds of hulless pumpkin ranged from 4.0 h (species C. pepo, cultivar Olianka) to 4.5 h (species C. pepo, cultivar Gleisdorfer Diamant). In turn, Rezig et al (2012) reported that the oxidative stability of oil pressed from seeds of C. maxima reached 18.61 h. Noteworthy is, however, the fact that Gorjanović et al (2011) were oxidizing the sample at a temperature of 100°C, Rezig et al (2012) at a temperature of 120°C.…”

“…Oil extracted from the seeds of different pumpkin species and from different growing regions significantly differs in terms of bioactive compound content. They may contain 647-971 mg/kg tocopherols (Fruhwirth et al 2003), 718-898 mg/100 g sterols (Rabrenović et al 2014), and 529-788 mg/100 g squalene (Gorjanović et al 2011). Apart from non-lipid bioactive components, the value of pumpkin seed oil is enhanced by a nutritionally beneficial composition of fatty acids.…”

Chemical Composition and Oxidative Stability of Roasted and Cold Pressed Pumpkin Seed Oils

“…Pumpkin seed oil is often noted for its high antioxidant activity and oxidative stability [34,35]. Considering that the production process of pumpkin seed oil includes roasting at high temperatures, such oxidative stability is not in accordance with the general notion that higher processing temperatures decrease oxidative stability.…”

Formulation and Characterization of Novel Nanostructured Lipid Carriers with Photoprotective Properties Made from Carnauba Wax, Beeswax, Pumpkin Seed Oil, and UV Filters

“…Furthermore, Lacatusu et al () and Badea et al () reported the presence of synergistic effect of plant seed oil with UV filters that can help to reduce the usage of synthetic UV filters in the production of sunscreen products. Rabrenovic et al (), Gorjanovic et al (), and Lacatusu et al () claimed PSO contains high amount of monounsaturated fatty acids, tocopherols, and phytosterols as well as squalene, which possess high potential in medical and nutritional purposes. Besides, the polyphenols present may benefit for photoprotection and inhibition of photocarcinogen (Afaq and Katiyar, ).…”