Cold plasma on full-thickness cutaneous wound accelerates healing through promoting inflammation, re-epithelialization and wound contraction

Abstract

We investigated cold plasma effects on acute wounds of mice. The mice were classified into experimental and control groups. In the former, wounds were treated using cold plasma once daily for 1 minute, and then covered with hydrocolloid dressing; wounds in the control were left to heal under hydrocolloid dressing. Daily evaluation was conducted for 15 days. General and specific staining was applied to evaluate re-epithelialization, neutrophil, macrophage, myofibroblast and transforming growth factor beta. It was found that cold plasma accelerated wound healing by one day. Plasma may promote the late phase of inflammation, accelerate reepithelialization and increase wound contraction.3

A Simple Technique to Improve Contractile Effect of Cold Plasma Jet on Acute Mouse Wound by Dropping Water

“…It has recently been shown that O 2 and ROS may trigger this mechanism . On the basis of histological study of a mouse model mimicking a clinical setting, it was reported that daily treatment with a cold plasma jet for 1 min on a full‐thickness wound accelerated its healing through the promotion of wound contraction, among others . In this previous study, wound size on Day 7 in the cold plasma‐treated group was macroscopically significantly smaller than that in the control group, but there was no statistically significant difference regarding the mean myofibroblast count.…”

“…Considering that wound healing is a complex living process consisting of orchestrated events communicated by collaborative biological substances and that cold plasma may be able to intervene at the microenvironmental level, it is not easy to identify and evaluate a single factor related to it. However, on the basis of macroscopic observation, it has been shown that the surface of the wound was drier after cold plasma treatment than that in a control …”

Improvement of cutaneous microcirculation by cold atmospheric plasma (CAP): Results of a controlled, prospective cohort study

“…Furthermore, immediate bacteria reduction in leg ulcers has been shown after a single use of CAP, but later an increase in bacteria density was observed (Ulrich et al, 2015). In mice repetitive treatments significantly increased the rate of wound closure (Ermolaeva et al, 2011;Nasruddin et al, 2014). Therefore, repeated applications have to be investigated in future studies to determine the impact on antimicrobial effects and wound healing in different types of human wounds, also regarding patients with a higher BMI.…”

“…Moreover, wound healing was accelerated in an in vivo model by an increase of IL-6, IL-8, MCP-1, TGF-b1/2, collagen type I, and alpha-SMA observed after a 2-min treatment (Arndt et al, 2013). Other animal models showed that CAP promoted wound healing in a repetitive application setting (Nasruddin et al, 2014;Ermolaeva et al, 2011). In studies evaluating CAP in humans, accelerated wound healing was observed with chronic wounds (Isbary et al, 2013;Brehmer et al, 2015) and at skin graft donor sites (Heinlin et al, 2013).…”

Plasma‐activated water to improve the stress tolerance of barley

“…In the beginning, studies focused on medical tools sterilization (bacteria, virus, and protein inactivation), applying different low‐pressure and also nonthermal high‐pressure discharge systems for different types of surfaces. The efficiency of atmospheric pressure plasmas in bacteria inactivation has turned attention towards therapeutical applications, such as wound‐cleaning related to the wound‐healing process, and further on to cancer therapy . In therapeutical applications, the plasma is in contact with body fluids, whereas in the cell‐culture experiments, with physiological solutions or cell‐culture media.…”