Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold

Zhu, Xinzhou; Bührer, Christoph; Wellmann, Sven

doi:10.1007/s00018-016-2253-7

Cited by 175 publications

(197 citation statements)

References 210 publications

(301 reference statements)

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“…At lower temperatures, growth of the grp1D strain was affected even more strongly and it exhibited an altered colony morphology ( Fig EV1H). This was consistent with a potential function in cold stress response, similar to the plant and human orthologues [20,21]. Furthermore, colony growth of the grp1D strain was strongly reduced upon treatment with inhibitors of cell wall biosynthesis, such as calcofluor-white (CFW) or Congo red (CR) [23,24].…”

Section: Loss Of the Glycine-/glutamine-rich Protein Grp1 Affects Hypsupporting

confidence: 76%

“…We identified the potential RBP glycine-rich protein 1 (Grp1; UMAG_02412), which carries an Nterminal RNA recognition motif (RRM) domain followed by a short C-terminal region rich in glycine and glutamine (GQ-rich; Figs 1A and EV1B-D). The protein was similar to other small RRM proteins, such as human CIRBP or RBM3 and plant RBG7 (AtGRP7), all previously described as global stress regulators ( Fig 1A) [20,21].…”

Section: Loss Of the Glycine-/glutamine-rich Protein Grp1 Affects Hypsupporting

confidence: 66%

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The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs

et al. 2018

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RNA‐binding proteins (RBPs) determine spatiotemporal gene expression by mediating active transport and local translation of cargo mRNAs. Here, we cast a transcriptome‐wide view on the transported mRNAs and cognate RBP binding sites during endosomal messenger ribonucleoprotein (mRNP) transport in Ustilago maydis. Using individual‐nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP), we compare the key transport RBP Rrm4 and the newly identified endosomal mRNP component Grp1 that is crucial to coordinate hyphal growth. Both RBPs bind predominantly in the 3′ untranslated region of thousands of shared cargo mRNAs, often in close proximity. Intriguingly, Rrm4 precisely binds at stop codons, which constitute landmark sites of translation, suggesting an intimate connection of mRNA transport and translation. Towards uncovering the code of recognition, we identify UAUG as specific binding motif of Rrm4 that is bound by its third RRM domain. Altogether, we provide first insights into the positional organisation of co‐localising RBPs on individual cargo mRNAs.

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Section: Loss Of the Glycine-/glutamine-rich Protein Grp1 Affects Hypsupporting

confidence: 76%

Section: Loss Of the Glycine-/glutamine-rich Protein Grp1 Affects Hypsupporting

confidence: 66%

The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs

et al. 2018

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“…RBM3 might act as a chaperone to ensure basal protein translation under hypothermic conditions. Moreover, RBM3 seems to have a regulatory function in the expression of microRNAs, although its exact function remains unclear 38–40. It has been demonstrated that RBM3 is able to mitigate cell death due to neurodegenerative diseases 25,41.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotection via RNA-binding protein RBM3 expression is regulated by hypothermia but not by hypoxia in human SK-N-SH neurons

Rosenthal¹,

Tong²,

Walker³

et al. 2017

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ObjectiveTherapeutic hypothermia is an established treatment for perinatal asphyxia. Yet, many term infants continue to die or suffer from neurodevelopmental disability. Several experimental studies have demonstrated a beneficial effect of mild-to-moderate hypothermia after hypoxic injury, but the understanding of hypothermia-induced neuroprotection remains incomplete. In general, global protein synthesis is attenuated by hypothermia, but a small group of RNA-binding proteins including the RNA-binding motif 3 (RBM3) is upregulated in response to cooling. The aim of this study was to establish an in vitro model to investigate the effects of hypoxia and hypothermia on neuronal cell survival, as well as to examine the kinetics of concurrent cold-shock protein RBM3 gene expression.MethodsExperiments were performed by using human SK-N-SH neurons exposed to different oxygen concentrations (21%, 8%, or 0.2% O2) for 24 hours followed by moderate hypothermia (33.5°C) or normothermia for 24, 48, or 72 hours. Cell death was determined by quantification of lactate dehydrogenase and neuron-specific enolase releases into the cell cultured medium, and cell morphology was assessed by using immunofluorescence staining. The regulation of RBM3 gene expression was assessed by reverse transcriptase-quantitative polymerase chain reaction and Western blot analysis.ResultsExposure to hypoxia (0.2% O2) for 24 hours resulted in significantly increased cell death in SK-N-SH neurons, whereas exposure to 8% O2 had no significant impact on cell viability. Post-hypoxia treatment with moderate hypothermia for 48 or 72 hours rescued the neurons from hypoxia-induced cell death. Moreover, exposure to severe hypoxia led to observable cell swelling, which was also attenuated by moderate hypothermia. Finally, moderate hypothermia but not hypoxia led to the induction of RBM3 expression on both transcriptional and translational levels.ConclusionModerate hypothermia protects neurons from hypoxia-induced cell death. The expression of the cold-shock protein RBM3 is induced by moderate hypothermia and could be one possible mediator of hypothermia-induced neuroprotection.

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“…Although we cannot completely exclude that some of the differentially expressed genes were stimulated by hypothermia, preclinical evidence has shown that a reduction by 4–10°C for over 6 h was required to influence gene expression in animal models [28-32]. Moreover, none of the hypothermia-inducible genes were found to be significantly differently expressed between neonatal encephalopathy and control babies [33]. Given that hypothermia is now the standard therapy for neonatal encephalopathy in high-income countries, it is no longer possible to examine gene expression profiles in a matched noncooled encephalopathic cohort in these settings.…”

Section: Discussionmentioning

confidence: 99%

Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy

et al. 2018

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Background: Variable responses to hypothermic neuroprotection are related to the clinical heterogeneity of encephalopathic babies; hence better disease stratification may facilitate the development of individualized neuroprotective therapies. Objectives: We examined if whole blood gene expression analysis can identify specific transcriptome profiles in neonatal encephalopathy. Material and Methods: We performed next-generation sequencing on whole blood RNA from 12 babies with neonatal encephalopathy and 6 time-matched healthy term babies. Genes significantly differentially expressed between encephalopathic and control babies were identified. This set of genes was then compared to the host RNA response in septic neonates and subjected to pathway analysis. Results: We identified 950 statistically significant genes discriminating perfectly between healthy controls and neonatal encephalopathy. The major pathways in neonatal encephalopathy were axonal guidance signaling (p = 0.0009), granulocyte adhesion and diapedesis (p = 0.003), IL-12 signaling and production in macrophages (p = 0.003), and hypoxia-inducible factor 1α signaling (p = 0.004). There were only 137 genes in common between neonatal encephalopathy and bacterial sepsis sets. Conclusion: Babies with neonatal encephalopathy have striking differences in gene expression profiles compared with healthy control and septic babies. Gene expression profiles may be useful for disease stratification and for developing personalized neuroprotective therapies.

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Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold

Cited by 175 publications

References 210 publications

The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs

The key protein of endosomal mRNP transport Rrm4 binds translational landmark sites of cargo mRNAs

Neuroprotection via RNA-binding protein RBM3 expression is regulated by hypothermia but not by hypoxia in human SK-N-SH neurons

Whole Blood Gene Expression Reveals Specific Transcriptome Changes in Neonatal Encephalopathy

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