Cold-induced stress increases the intensity of Chlamydia genital infection in mice

Belay, Tesfaye; Woart, Anthony

doi:10.1016/j.jmii.2012.06.002

Cited by 21 publications

(40 citation statements)

References 22 publications

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“…As shown in Table 1, there are greater numbers of chlamydia inclusion counts in the swabs of stressed mice compared to non-stressed mice, indicating that cold-induced stress increases the intensity of C. muridarum genital infection in mice. Our results are consistent with our previous findings of increased C. muridarum shedding in regions of the genital tract of stressed mice compared to non-stressed mice [59,60].…”

Section: Cold Water-induced Stress Increases the Intensity Of Chlamydsupporting

confidence: 93%

“…Application of cold water as a stressor in animal models, including mice, has resulted in changes in immune responses that correlate with the activity of the neuroendocrine system of corticosteroids and catecholamines [55][56][57][58][59][60]. Although stress is implicated as a risk factor for various infections, its effect on chlamydia genital infection is unknown.…”

Section: Trachomatismentioning

confidence: 99%

“…Although stress is implicated as a risk factor for various infections, its effect on chlamydia genital infection is unknown. We have previously shown that cold-water stress induces the production of catecholamines, which may play a critical role in the modulation of the immune system leading to increased intensity of C. muridarum genital infection [59,60]. Furthermore, we have shown that supplement of NE to in vitro exerts an immunosuppressive effect on splenic T cell production of cytokines, decreased C. muridarum shedding in the genital tract of β1Adr/β2Adr KO mice, and severe pathology with a higher rate of infertility in C. muridarum infected mice [60].…”

Section: Trachomatismentioning

confidence: 99%

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Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Belay

Martin

Brown

et al. 2019

Preprint

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Running Title: Stress and Chlamydia muridarum genital infection ABSTRACTA mouse model to study the effect of cold-induced stress on Chlamydia muridarum genital infection and immune response has been developed in our laboratory. Our previous results show that cold-induced stress increases the intensity of chlamydia genital infection, but little is known about the effect of cold-induced stress on differentiations and activities of T cell subpopulations and bone marrow derived dendritic cells (BMDCs). The factors that regulate the production of T helper 1 (Th1) or T helper 2 (Th2) cytokines is not clear. The objective of this study was to examine whether cold-induced stress modulates the expression of transcription factors and hallmark cytokines of Th1 and Th2 or differentiation of BMDCs during C. muridarum genital infection in mice. Our results show that mRNA level of beta2-adrenergic receptor (2-AR) compared to 1-AR and 3-AR was high in mixed population of CD4+ T cells and BMDCs.Further, decreased expression of T-bet and low level of interferon-gamma (IFN-) production and increased expression of GATA-3 and interleukin-4 (IL-4) production in CD4+ T of stressed mice was observed. Exposure of BMDCs to feroterol (2-AR agonist) or ICI,118551 ( 2-AR antagonist), respectively, revealed significant stimulation or inhibition of 2-AR in stressed mice. Moreover, co-culturing of mature BMDC and naïve CD4+ T cells resulted in increased production of IL-4, IL-10, and IL-17 in culture supernatants, suggesting that stimulation of 2-AR leads to the increased production of Th2 cytokines. Overall, our results show for the first time that cold-induced stress is able to modulate the pattern of Th1 and Th2 cytokine environment, suggesting that it promotes the differentiation to Th2 rather than Th1 by the overexpression of GATA-3 correlated with elevated production of IL-4, IL-10, in contrast to a low expression of T-bet correlated with less IFN- secretion in the mouse model.

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Section: Cold Water-induced Stress Increases the Intensity Of Chlamydsupporting

confidence: 93%

Section: Trachomatismentioning

confidence: 99%

Section: Trachomatismentioning

confidence: 99%

See 1 more Smart Citation

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Belay

Martin

Brown

et al. 2019

Preprint

Self Cite

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show abstract

“…The increase in Firmicutes bacteria during cold exposure is likely due to decreased immunity. Cold stress is known to (a) reduce both innate and adaptive immunity by decreasing activity and proliferation of immune cells (Cao and Lawrence, 2002;Cheng et al, 1990;Karp, 2012;Shephard and Shek, 1998) and (b) increase several stress hormones, including glucocorticoids (Belay and Woart, 2013;Karp, 2012). Three-week cold stress in rats leads to significantly reduced intestinal epithelial cell proliferation and promotes intestinal inflammation (Kaushik and Kaur, 2005).…”

Section: Ucp1 Independent Thermogenesis a Cost-inefficient Means To mentioning

confidence: 99%

Immunity-Based Evolutionary Interpretation of Diet-Induced Thermogenesis

2016

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Diet-induced thermogenesis (DIT) has often been argued to be a physiological defense against obesity, but no empirical proof of its effectiveness in limiting human body weight gain is available. We here propose an immune explanation of DIT-i.e., that it results from the coevolution of host and gut microbiota (especially Firmicutes) that ferment ingested food and proliferate, causing periodic, vagally mediated increases in thermogenesis aimed at curtailing their expansion. Because of this evolutionary adaptive significance related to the immune system, DIT is not effective as an "adaptation" to maintain a certain body mass. Were DIT an effective adaptation to prevent obesity, the current obesity epidemic might not have occurred.

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“…For example, cold stress impaired humoral immunity in mice (Cichoné t al., 2002), deer mice (Peromyscus maniculatus) and grey-sided voles (Clethrionomys rufocanus) (Kusumoto and Saitoh, 2008). It also increased the intensity of genital infection in mice (Belay and Woart, 2013). Nevertheless, cold stress did not affect cellular immunity in deer mice (Demas and Nelson, 1998), or the proliferation of lymphocytes and the ability of macrophages to bind bacterial lipopolysaccharide in goldenmantled ground squirrels (Spermophilus lateralis) (Maniero, 2002(Maniero, , 2005.…”

Section: Introductionmentioning

confidence: 99%

Effect of temperature and food restriction on immune function in striped hamsters (Cricetulus barabensis)

Tian

2017

Journal of Experimental Biology

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Small mammals in temperate areas face seasonal fluctuations of temperature and food availability, both of which may influence their immune responses, which are critical to survival. In the present study, we tested the hypothesis that low temperature and food restriction suppress immune function in striped hamsters (Cricetulus barabensis). Thirty-seven adult male hamsters were randomly assigned to warm (23±1°C) and cold (5±1°C) treatment groups, which were further divided into fed and food-restricted groups. Body mass was not affected by cold stress, food restriction or the interaction cold stress×food restriction. Cold stress decreased total body fat mass, haematological parameters including white blood cells, lymphocytes and neutrophilic granulocytes, and immunoglobin (Ig) M titres 5 days after injecting keyhole limpet haemocyanin (KLH). However, cold temperature increased bacterial killing capacity, indicative of innate immunity, and did not affect the mass of the thymus and spleen, intermediate granulocytes, the phytohaemagglutinin (PHA) response and the levels of blood glucose and serum leptin. Corticosterone concentration was affected significantly by the interaction cold stress×food restriction but not by cold stress or food restriction alone. Food restriction reduced thymus mass, but other immunological parameters including body fat mass, spleen mass, haematological parameters, innate immunity, PHA response, the titres of IgM and IgG, and the levels of blood glucose and serum leptin were all not affected by food restriction or the interaction cold stress×food restriction. Innate immunity was positively correlated with leptin levels, whereas no significant correlations were observed in the levels of blood glucose, serum leptin, corticosterone and all the detected immune parameters. Our results show that cold stress suppressed humoral immunity but enhanced innate immunity and did not affect cellular immunity in striped hamsters. Most immunological indices were not influenced by food restriction. Blood glucose, leptin and corticosterone could not explain the changes of innate, cellular and humoral immunity upon cold stress or food restriction in striped hamsters.

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Cold-induced stress increases the intensity of Chlamydia genital infection in mice

Cited by 21 publications

References 22 publications

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Immunity-Based Evolutionary Interpretation of Diet-Induced Thermogenesis

Effect of temperature and food restriction on immune function in striped hamsters (Cricetulus barabensis)

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