Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

Koç, İbrahim; Akdemir, Hülya; Onay, Ahmet; Çiftçi, Yelda Özden

doi:10.1007/s11738-014-1610-0

Cited by 18 publications

(12 citation statements)

References 55 publications

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“…The best results have been observed when storage was performed in darkness for Malus (Orlikowska 1992), Pistacia (Koç et al 2014), Populus (Hausman et al 1994), and Quercus (Romano and Martins-Loução 1999). Similar results were reported for S. macrophyllus where light affected negatively culture viability, multiplication rate and rooting response.…”

Section: Discussionsupporting

confidence: 76%

“…Glass conducts heat better than polycarbonate, so perhaps plant material in the jars was more chilled than the material stored in Magenta boxes. Polycarbonate magenta boxes were also successfully used for cold storage of Solanum tuberosum, Prunus virginiana (Pruski et al 2000) and for Pistacia lentiscus (Koç et al 2014). Similar results were obtained by Kovalchuk et al (2009) for two varieties of apple, where shoots stored in semi-permeable plastic bags Fig.…”

Section: Discussionmentioning

confidence: 80%

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In vitro slow growth storage of Senecio macrophyllus shoots

2015

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Advances in biotechnology, especially in the field of in vitro culture techniques, led to the development of procedures that can be used as an excellent tool in plant conservation. The present study describes in vitro conservation of Senecio macrophyllus by slow-growth storage. Various sugar treatments, concentration of abscisic acid (ABA), light intensity and type of containers were tested. Viability and proliferation rate of shoots were evaluated 4 weeks after regrowth. The results obtained showed that polycarbonate boxes were a better type of containers for storage of S. macrophyllus shoots than glass vessels. Light negatively affected culture viability, multiplication rate and rooting response. In the case of Senecio macrophyllus the addition of ABA to storage medium stimulated survival and higher proliferation of shoots during regrowth in optimal conditions in comparison to shoots stored on medium without ABA. Shoots of S. macrophyllus were effectively stored for 6 months. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 80%

In vitro slow growth storage of Senecio macrophyllus shoots

2015

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“…The dendrogram of IRAP data was examined in order to evaluate phylogenetic relations and it was shown that 3 main groups and 1 genotype (LBB) were separated, and retrotransposon mobilities resulting in high polymorphism were affected by the geographical locations of the plant samples. The results of Kılınç et al (2014) and Koç et al (2014) on P. lentiscus based on IRAP and AFLP techniques were supported with the findings of amplification data obtained in our present study in relation to the LTR2 region. Kırdök & Çiftçi (2016) used iPBS, REMAP and IRAP techniques of retrotransposon markers for genetic diversity analysis of Pistacia genus.…”

Section: Discussionsupporting

confidence: 91%

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Serttaş¹,

Özcan²

2018

Trakya University Journal of Natural Sciences

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In this study, intra-specific variations in naturally growing and cultivated mastic tree (Pistacia lentiscus L.) samples obtained from western parts of Turkey were examined using ISSR and IRAP marker techniques. Samples from Crete and Chios were also included in the study. Morphological measurements of some leaf characteristics of the samples were performed and the measured data was evaluated statistically with a Pearson Correlation analysis to reveal the correlations between character pairs. ISSR primers produced 81 bands between 161-1884bp with 96.3% polymorphism and IRAP primers produced 72 bands between 124-2027bp with 91.67% polymorphism. Polymorphism information content (PIC) values were 0.458 and 0.418 for ISSR and IRAP, respectively. Genetic similarity matrix was examined with Jaccard's coefficient. Maximum similarity was found between the Cretan samples (LG2 and LG3) with the ISSR analysis (0.933) and between L25A (C1, Bodrum) and L29A (C1, Milas) with the IRAP analysis (0.593). Unweighted pair group method with arithmetic mean (UPGMA) dendrogram was divided into 12 and 4 groups by ISSR and IRAP methods, respectively. Specimens were segregated on 3 main different clusters by the Principal Component Analysis (PCA) based on the combined marker systems. The results showed that P. lentiscus has very high ratios of intraspecific variation. The present work is an original study in terms of large sampling including wild genotypes, cultivated specimen, Chios and Cretan varieties, use of ISSR and IRAP combination, determination of relations between culture and wild genotypes and the use of Bagy-1 retrotransposons in intraspecific polymorphism. This study may be considered as a reference study for studies on gene pools of P. lentiscus and phylogenetic relationships within the species and may contribute to species concept and agricultural breeding programs.Özet: Bu çalışmada, ISSR ve IRAP markör teknikleri kullanılarak Türkiye'nin batı kesiminde doğal olarak yetişen ve kültürü yapılan sakız ağacı (Pistacia lentiscus L.) örneklerindeki tür içi varyasyon analizi yapılmıştır. Girit ve Sakız Adası örnekleri de çalışmaya dahil edilmiştir. Bazı yaprak özelliklerinin morfolojik ölçümleri gerçekleştirilmiş ve aralarındaki korelasyon Pearson Korelasyon analizi ile belirlenmiştir. ISSR primerleri 161-1884bç arasında %96,3 polimorfizm ile 81 bant üretmiştir. IRAP primerleri ise 124-2027bç arasında %91,67 polimorfizm ile 72 bant üretmiştir. Polimorfizm bilgi içeriği (PIC) değerleri 0,458 (ISSR) ve 0,418 (IRAP) arasında bulunmuştur. Genetik benzerlik matriksleri Jaccard katsayısıyla oluşturulmuştur. ISSR sonuçlarında en yüksek benzerlik Girit örnekleri (LG2 ve LG3) arasında (0,933) ve IRAP sonuçlarında ise L25A (C1, Bodrum) ve L29A (C1, Milas) arasında bulunmuştur. UPGMA yöntemiyle kurulan dendrogramlarda sırasıyla ISSR için 12 grup ve IRAP için 4 grup ayrılmıştır. Her iki markör sistemi için ortak kurulan temel bileşen analizi (PCA) grafiğinde 3 farklı küme oluşmuştur. Sonuçlar göstermiştir ki, P. lentiscus yüksek oranlarda tü...

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“…However, slow growth storage resulted in a significant DNA methylation changes. In contrast, high propability of genetic instability was observed after 12 months of storage at 4° of Pistacia lentiscus (Koç et al 2014).…”

Section: Discussionmentioning

confidence: 80%

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

Kamińska

Gołębiewski

Tretyn

et al. 2017

Plant Cell Tiss Organ Cult

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The aim of this paper was to develop a protocol for efficient storage of artificial seeds of Taraxacum pieninicum, critically endangered Asteraceae species. Storage under reduced light conditions or in the darkness was tested on a basis of synthetic seeds ability to conversion and post-storage regrowth of shoot tips. The results indicated that synseeds obtained from shoot tips of T. pieninicum can be stored at 4 °C even for 12 months without subculture. The light is a stress factor during storage what was manifested by numerous necrosis and decreased shoots ability to proliferate in optimal growth conditions in 1st subculture. Additionally our results showed that the storage does not produce genetic variation at the resolution provided by the flow cytometry and RAPD analysis.

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Cold-induced genetic instability in micropropagated Pistacia lentiscus L. plantlets

Cited by 18 publications

References 55 publications

In vitro slow growth storage of Senecio macrophyllus shoots

In vitro slow growth storage of Senecio macrophyllus shoots

INTRASPECIFIC VARIATIONS STUDIED BY ISSR AND IRAP MARKERS IN MASTIC TREE (Pistacia lentiscus L.) FROM TURKEY

Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions

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