Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Menon, Akshay; Funnekotter, Bryn; Kaczmarczyk, Anja; Bunn, Eric; Turner, Shane R.; Mancera, Ricardo L.

doi:10.1007/s11240-014-0538-9

Cited by 11 publications

(5 citation statements)

References 45 publications

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“…As recommended by Normah et al [135], once tissue has been established in vitro, the effect on survival and regrowth of pretreatments, loading solutions, cryoprotectants, exposure to liquid nitrogen, rewarming, and unloading solutions, will need to be investigated sequentially. Characterizing changes in sugar content and cell membrane lipid composition [136,137], the activity of anti-oxidant enzymes [138], changes in oxidative status [139], and changes in metabolic rates [140] and mitochondrial function [141] in response to the application of pre-treatments and cryoprotectants may aid in identifying the most appropriate protocol for a given species. Tools such as differential scanning calorimetry [142,143] could be utilized to optimize tissue desiccation strategies such as vacuum infiltration vitrification, cryo-mesh or flash drying [144,145].…”

Section: Discussionmentioning

confidence: 99%

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Hardstaff

Sommerville

Funnekotter

et al. 2022

Plants

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The Myrtaceae is a very large and diverse family containing a number of economically and ecologically valuable species. In Australia, the family contains approximately 1700 species from 70 genera and is structurally and floristically dominant in many diverse ecosystems. In addition to threats from habitat fragmentation and increasing rates of natural disasters, infection by myrtle rust caused by Austropuccinia psidii is of significant concern to Australian Myrtaceae species. Repeated infections of new growth have caused host death and suppressed host populations by preventing seed set. Although most Myrtaceae species demonstrate orthodox seed storage behavior, exceptional species such as those with desiccation sensitive seed or from myrtle rust-suppressed populations require alternate conservation strategies such as those offered by cryobiotechnology. Targeting seven key Australian genera, we reviewed the available literature for examples of cryobiotechnology utilized for conservation of Myrtaceae. While there were only limited examples of successful cryopreservation for a few genera in this family, successful cryopreservation of both shoot tips and embryonic axes suggest that cryobiotechnology provides a viable alternative for the conservation of exceptional species and a potential safe storage method for the many Myrtaceae species under threat from A. psidii.

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Section: Discussionmentioning

confidence: 99%

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Hardstaff

Sommerville

Funnekotter

et al. 2022

Plants

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“…Another alternative to improve tolerance to freezing among temperate and subtropical species is the conditioning of plants to low temperatures, known as cold acclimation and cold-hardening (Reed & Uchendu, 2008;Sinha et al, 2015). Cold acclimation likely activates genes that promote membrane stability (Kaczmarczyk et al, 2012;Menon et al, 2014). This technique could be used in the cryopreservation process of Araucaria angustifolia EA as it is a subtropical species.…”

Section: Discussionmentioning

confidence: 99%

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

Frizzo

Quoirin

2018

Pesq. flor. bras.

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Araucaria angustifolia (Bertol.) O. Kuntze é uma das espécies nativas do Brasil com maior importância na Região Sul. No entanto, suas sementes são recalcitrantes, constituindo um obstáculo para a sua conservação em longo prazo, sendo a criopreservação uma alternativa viável para o armazenamento de seu germoplasma. Os eixos embrionários (EE) excisados das sementes de araucária foram encapsulados, desidratados e submetidos a dois métodos de criopreservação: o resfriamento rápido, congelando em nitrogênio líquido (NL) durante 2 h, e o pré-resfriamento a - 40 °C, seguido do congelamento em nitrogênio líquido durante 2 h. Posteriormente, os EE foram descongelados rapidamente e avaliados quanto à integridade do DNA pelo teste bioquímico do tetrazólio, germinação in vitro e ocorrência de oxidação. Tanto o DNA dos EE que não foram criopreservados quanto o daqueles que foram criopreservados mantiveram sua integridade. Os resultados do teste de tetrazólio indicaram que a maioria dos EE desidratados pelo congelamento rápido permaneceu viável. Após 15 dias de cultivo in vitro, os EE não germinaram e apresentaram sinais de oxidação. Os métodos de encapsulamento-desidratação seguidos de congelamento rápido são promissores para a criopreservação de EE de araucária, como demonstrado pelo teste de tetrazólio e da manutenção da integridade total do DNA.

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“…Preconditioning is the method of exposing plants to an abiotic stress before cryopreservation to alter its cellular properties (Kaczmarczyk et al 2012). Temperature preconditioning is commonly used as a method to increase cell membrane stability at low temperatures through increased levels of unsaturated acyl chains (Menon et al 2014). Studies on two Australian species (Grevillea scapigera and Loxocarya cinerea) exposed to 20/10 C alternating temperature preconditioning resulted in a decrease in common acyl chains (16 : 0, 18 : 0, 18 : 1 and 18 : 2) with an increase in a-linoleyl tails (18 : 3) (Funnekotter et al 2013).…”

Section: The Role Of Cell Membranes In Cryopreservationmentioning

confidence: 99%

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

et al. 2019

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An alarming proportion of Australia’s unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries regardless of best intentions. Ex situ conservation is currently limited to botanic garden living collections and seed banking, with in vitro and cryopreservation technologies still being developed to address ex situ conservation of species not amenable to conventional storage. Cryopreservation (storage in liquid nitrogen) has been used successfully for long-term biosecure storage of shoot tips of several species of threatened Australian plants. We present a case for building on this research and fostering further development and utilisation of cryopreservation as the best means of capturing critical germplasm collections of Australian species with special storage requirements (e.g. recalcitrant-seeded taxa and species with short-lived seeds) that currently cannot be preserved effectively by other means. This review highlights the major issues in cryopreservation that can limit survival including ice crystal damage and desiccation, toxicity of cryoprotective agents, membrane damage, oxidative stress and mitochondrial function. Progress in understanding and mitigating these stresses is vital for advancing cryopreservation for conservation purposes.

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Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Cited by 11 publications

References 45 publications

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Myrtaceae in Australia: Use of Cryobiotechnologies for the Conservation of a Significant Plant Family under Threat

Viability of embryonic axes of Araucaria angustifolia after freezing using two cryopreservation methods

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

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