Cold atmospheric plasma inhibits the growth of Candida albicans by affecting ergosterol biosynthesis and suppresses the fungal virulence factors in vitro

Rahimi-Verki, Neda; Shapoorzadeh, Atena; Razzaghi-Abyaneh, Mehdi; Atyabi, Seyed-Mohammad; Shams-Ghahfarokhi, Masoomeh; Jahanshiri, Zahra; Gholami-Shabani, Mohammadhassan

doi:10.1016/j.pdpdt.2015.12.007

Cited by 32 publications

(26 citation statements)

References 27 publications

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“…This study showed that the He-CAPPJ jet did not influence protease and phospholipase activity of C. albicans SC 5314. Conversely, Rahimi-Verki et al [42] observed a reduction of enzymatic activity in C. albicans ATCC 10231 after exposure to a He/O 2 plasma jet for 90 s. The contrast in the findings may be associated to significant differences in the plasma sources, C. albicans strains and volume of testing suspension between the studies. To our knowledge, there is no other study describing the effect of plasma jets on C. albicans enzymatic activity that could be used for further comparative analyses.…”

Section: Discussionmentioning

confidence: 91%

“…The effect of cold atmospheric pressure plasma jets on C. albicans has been previously described [20,29,30]. However, the effects of C-APPJ on C. albicans virulence factors have not been fully evaluated with only sparse reports on the reduction of exoenzymes production [42]. Considering that the occurrence of the disease depends directly on the expression of fungal virulence factors, the interference on these traits may contribute significantly to the infection control.…”

Section: Discussionmentioning

confidence: 99%

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Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Borges

Nishime

Kostov

et al. 2017

Clinical Plasma Medicine

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The goal of this study was to analyze the effects of cold atmospheric pressure plasma jet operated with Helium (He-CAPPJ) on C. albicans virulence traits and biofilms. The study also aimed to evaluate the cytotoxicity of He-CAPPJ to fibroblasts. Methods: The effective operational parameters of He-CAPPJ against C. albicans SC 5314 were determined. Subinhibitory times of exposure were used to evaluate the effect of He-CAPPJ on morphogenesis (yeast-hyphae transition), adherence to epithelial cells, and exoenzymes production. Cytotoxicity to Vero cells was also assessed by MTT assay. Results: A promising effect of He-CAPPJ on morphogenesis was observed, with an almost 40 times reduction of the filamentation rate comparing to the non-exposed group. He-CAPPJ was able to reduce C. albicans adherence and to decrease fungal biofilm viability. No effect on exoenzymes production was observed. Conclusion: He-CAPPJ was able to attenuate fungal adherence and morphogenesis with low cytotoxicity to fibroblasts. Therefore, He-CAPPJ has potential to be applied as an adjuvant in the treatment of C. albicans superficial infections. Additionally, the ability of C. albicans to develop resistance against antifungals is remarkable [1,2]. Although azoles and polyenes are drugs of choice for the treatment of these infections, they are not always effective [7,8]. Besides, other adverse effects may occur, such as toxicity and interactions with other drugs [9], limiting the number of therapeutic alternatives. For this reason, the search for new antifungal therapies is of utmost importance. Cold atmospheric pressure plasma (CAPP) has emerged as a promising technology in recent years. Due to their low operational cost, absence of residual and toxic emissions and operation at room temperature, cold plasmas have drawn much attention for biomedical applications [10-12]. Among different atmospheric plasma sources, special interest has been driven to cold atmospheric pressure plasma jets (CAPPJs), since plasma plumes can be obtained in open space instead of confined volumes [13]. The plasma plume propagates into the surrounding environment and interacts with air molecules creating

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Borges

Nishime

Kostov

et al. 2017

Clinical Plasma Medicine

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“…However, this period of exposure is much lower than the period of 300 seconds, usually needed to affect the viability of C . albicans biofilms [ 22 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study

et al. 2018

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The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity.

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“…In recent years, the incidence of fungal infections especially Candida -related disorders termed generally as “candidiasis” has increased dramatically in many countries [ 8 ]. On the other hand, it is known that disseminated infections with C. albicans cause significant morbidity and mortality among immunocompromised individuals, such as Human Immunodeficiency Virus (HIV) patients, transplant recipients, and cancer patients [ 8 , 9 , 10 , 11 ]. Furthermore, progression of the yeast with increasing the resistance of therapeutic conventional medicine increased the need for the most effective treatment [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Candida Activity of Bursera morelensis Ramirez Essential Oil and Two Compounds, α-Pinene and γ-Terpinene—An In Vitro Study

et al. 2017

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The candidiasis caused by C. albicans is a public health problem. The abuse of antifungals has contributed to the development of resistance. B. morelensis has demonstrated antibacterial and antifungal activities. In this work the activity of the essential oil of B. morelensis was evaluated and for its two pure compounds with analysis of the different mechanisms of pathogenesis important for C. albicans. The essential oil was obtained by the hydro-distillation method and analyzed using GC–MS. The anti-Candida activity was compared between to essential oil, α-Pinene and γ-Terpinene. GC–MS of the essential oil demonstrated the presence of 13 compounds. The essential oil showed antifungal activity against four C. albicans strains. The most sensitive strain was C. albicans 14065 (MFC 2.0 mg/mL and MIC50 0.125 mg/mL) with α-Pinene and γ-Terpinene having MFCs of 4.0 and 16.0 mg/mL respectively. The essential oil inhibited the growth of the germ tube in 87.94% (8.0 mg/mL). Furthermore, it was observed that the essential oil diminishes the transcription of the gene INT1. This work provides evidence that confirms the anti-Candida activity of the B. morelensis essential oil and its effect on the growth of the germ tube and transcription of the gene INT1.

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Cold atmospheric plasma inhibits the growth of Candida albicans by affecting ergosterol biosynthesis and suppresses the fungal virulence factors in vitro

Cited by 32 publications

References 27 publications

Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Cold atmospheric pressure plasma jet modulates Candida albicans virulence traits

Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study

Anti-Candida Activity of Bursera morelensis Ramirez Essential Oil and Two Compounds, α-Pinene and γ-Terpinene—An In Vitro Study

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