Cold atmospheric plasma cancer treatment, direct <i>versus</i> indirect approaches

Malyavko, Alisa; Yan, Dayun; Wang, Qihui; Klein, Andrea; Patel, Khyati C.; Sherman, Jonathan H.

doi:10.1039/d0ma00329h

Cited by 40 publications

(33 citation statements)

References 131 publications

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“…Other significant benefit of plasma-based techniques for cancer therapy is their ability to be applied by two different approaches. Generally, in plasma medicine two treatment approaches are introduced using direct and indirect plasma sources [99,106]. In the former, the target is electrically grounded and thus any plasma current induced by charged plasma species can flow through it.…”

Section: Low Temperature Plasma Sources For Medical Applicationsmentioning

confidence: 99%

Recent Advances in Plasma-Based Cancer Treatments: Approaching Clinical Translation through an Intracellular View

Alizadeh

Ptasińska

2021

Biophysica

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Plasma medicine is a multidisciplinary field of research which is combining plasma physics and chemistry with biology and clinical medicine to launch a new cancer treatment modality. It mainly relies on utilizing low temperature plasmas in atmospheric pressure to generate and instill a cocktail of reactive species to selectively target malignant cells for inhibition the cell proliferation and tumor progression. Following a summarized review of primary in vitro and in vivo studies on the antitumor effects of low temperature plasmas, this article briefly outlines the plasma sources which have been developed for cancer therapeutic purposes. Intracellular mechanisms of action and significant pathways behind the anticancer effects of plasma and selectivity toward cancer cells are comprehensively discussed. A thorough understanding of involved mechanisms helps investigators to explicate many disputes including optimal plasma parameters to control the reactive species combination and concentration, transferring plasma to the tumors located in deep, and determining the optimal dose of plasma for specific outcomes in clinical translation. As a novel strategy for cancer therapy in clinical trials, designing low temperature plasma sources which meet the technical requirements of medical devices still needs to improve in efficacy and safety.

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Section: Low Temperature Plasma Sources For Medical Applicationsmentioning

confidence: 99%

Recent Advances in Plasma-Based Cancer Treatments: Approaching Clinical Translation through an Intracellular View

Alizadeh

Ptasińska

2021

Biophysica

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“…In addition, the selectivity of the CAPP-activated media was that the cancerous cell lines were more vulnerable to the oxidative stress caused by RONS than the normal cell lines, which were not so sensitive to this oxidative stress [ 30 ]. What is more, in the case of the application of the CAPP-activated media towards the cancerous cell lines, there was no physical effect on the cell lines associated with the damages caused by a gas flux (CAPP discharge gas) and the electromagnetic field (from a CAPP source) [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Cold Atmospheric Pressure Plasma-Treated Media on the Cell Viability, Motility, and Induction of Apoptosis in Human Non-Metastatic (MCF7) and Metastatic (MDA-MB-231) Breast Cancer Cell Lines

Terefinko

Dzimitrowicz

Bielawska‐Pohl

et al. 2021

IJMS

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Breast cancer remains the most common type of cancer, occurring in middle-aged women, and often leads to patients’ death. In this work, we applied a cold atmospheric pressure plasma (CAPP)-based reaction-discharge system, one that is unique in its class, for the production of CAPP-activated media (DMEM and Opti-MEM); it is intended for further uses in breast cancer treatment. To reach this aim, different volumes of DMEM or Opti-MEM were treated by CAPP. Prepared media were exposed to the CAPP treatment at seven different time intervals and examined in respect of their impact on cell viability and motility, and the induction of the apoptosis in human non-metastatic (MCF7) and metastatic (MDA-MB-231) breast cancer cell lines. As a control, the influence of CAPP-activated media on the viability and motility, and the type of the cell death of the non-cancerous human normal MCF10A cell line, was estimated. Additionally, qualitative and quantitative analyses of the reactive oxygen and nitrogen species (RONS), generated during the CAPP operation in contact with analyzed media, were performed. Based on the conducted research, it was found that 180 s (media activation time by CAPP) should be considered as the minimal toxic dose, which significantly decreases the cell viability and the migration of MDA-MB-231 cells, and also disturbs life processes of MCF7 cells. Finally, CAPP-activated media led to the apoptosis of analyzed cell lines, especially of the metastatic MDA-MB-231 cell line. Therefore, the application of the CAPP system may be potentially applied as a therapeutic strategy for the management of highly metastatic human breast cancer.

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“…On the other hand, directly CAP-treated cancer cells enter the activation state and become very sensitive to the cytotoxicity of long-lived reactive species like H 2 O 2 and NO 2 - [ 171 , 172 ]. These two cellular responses do not appear in indirect CAP treatment [ 173 ]. Whether bacteria also take part in these two cellular responses to CAP treatment is still unknown and requires dedicated studies.…”

Section: Cross-species Similaritiesmentioning

confidence: 99%

Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism

et al. 2021

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Cold atmospheric plasma (CAP) is a near-room-temperature, partially ionized gas composed of reactive neutral and charged species. CAP also generates physical factors, including ultraviolet (UV) radiation and thermal and electromagnetic (EM) effects. Studies over the past decade demonstrated that CAP could effectively induce death in a wide range of cell types, from mammalian to bacterial cells. Viruses can also be inactivated by a CAP treatment. The CAP-triggered cell-death types mainly include apoptosis, necrosis, and autophagy-associated cell death. Cell death and virus inactivation triggered by CAP are the foundation of the emerging medical applications of CAP, including cancer therapy, sterilization, and wound healing. Here, we systematically analyze the entire picture of multi-modal biological destruction by CAP treatment and their underlying mechanisms based on the latest discoveries particularly the physical effects on cancer cells.

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Cold atmospheric plasma cancer treatment, direct versus indirect approaches

Abstract: Cold atmospheric plasma (CAP), an ionized gas with near room temperature, shows a wide application in medicine. CAP is a tunable source of complex chemical components including many reactive species,...

Cited by 40 publications

References 131 publications

Recent Advances in Plasma-Based Cancer Treatments: Approaching Clinical Translation through an Intracellular View

Recent Advances in Plasma-Based Cancer Treatments: Approaching Clinical Translation through an Intracellular View

The Influence of Cold Atmospheric Pressure Plasma-Treated Media on the Cell Viability, Motility, and Induction of Apoptosis in Human Non-Metastatic (MCF7) and Metastatic (MDA-MB-231) Breast Cancer Cell Lines

Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism

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