Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism

Yan, Dayun; Malyavko, Alisa; Wang, Qihui; Ostrikov, Kostya; Sherman, Jonathan H.; Keidar, Michael

doi:10.3390/biomedicines9091259

Cited by 26 publications

(28 citation statements)

References 173 publications

(325 reference statements)

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“…LTP produces oxidative stress. RONS, charged particles, ultraviolet radiation and electromagnetic fields, all of which can play a role and produce synergy in the therapy of cancer [41]. Among them, RONS is the most important element of LTP to kill tumors.…”

Section: The Oxidative Stress Toxicity Of Ltpmentioning

confidence: 99%

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Research Advances and Application Prospect of Low-Temperature Plasma in Tumor Immunotherapy

2021

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As an emerging technology, low-temperature plasma (LTP) is widely used in medical fields such as sterilization, wound healing, stomatology, and cancer treatment. Great achievements have been made in tumor therapy. In vitro and in vivo studies have shown that LTP has anti-tumor effects, and LTP is selective to tumor cells. Studies in recent years have found that LTP can activate dendritic cells (DC), macrophages, T cells, and other immune cells to achieve anti-tumor effects. This paper reviews the current status of tumor immunotherapy, the application of LTP in antitumor therapy, the activation of antitumor immunity by LTP, the possible mechanism of LTP in antitumor immunity, and meanwhile analyses the prospect of applying LTP in tumor immunotherapy.

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Section: The Oxidative Stress Toxicity Of Ltpmentioning

confidence: 99%

“…The uptake of tumor antigens by dendritic cells was increased. Then, DCs presented tumor antigens to anti-tumor T cells [41] (Figure 3). Lin [49]…”

Section: Ltp Induces Immunogenic Cell Deathmentioning

confidence: 99%

Research Advances and Application Prospect of Low-Temperature Plasma in Tumor Immunotherapy

2021

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“…Therapies targeting TRAIL (TNF (tumor necrosis factor)-related apoptosis-inducing ligand) 8 and cyclin-dependent kinases (CDK) or cell cycle regulators 9 have been used with limited success. In recent years Cold atmospheric plasma (CAP) technology that utilizes ionized gas to selectively induce apoptosis in cancer cells has shown very encouraging results 10 – 13 . Preclinical In vivo studies in mouse models for various cancers, CAP treatment have demonstrated to effectively reduce tumor growth rate and induce cancer cell death.…”

Section: Introductionmentioning

confidence: 99%

BCL2A1 regulates Canady Helios Cold Plasma-induced cell death in triple-negative breast cancer

Murthy¹,

Cheng²,

Zhuang³

et al. 2022

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Breast cancer is the leading cause of cancer death among women. Triple-negative breast cancer (TNBC) has a poor prognosis and frequently relapses early compared with other subtypes. The Cold Atmospheric Plasma (CAP) is a promising therapy for prognostically poor breast cancer such as TNBC. The Canady Helios Cold Plasma (CHCP) induces cell death in the TNBC cell line without thermal damage, however, the mechanism of cell death by CAP treatment is ambiguous and the mechanism of resistance to cell death in some subset of cells has not been addressed. We investigate the expression profile of 48 apoptotic and 35 oxidative gene markers after CHCP treatment in six different types of breast cancer cell lines including luminal A (ER+ PR+/−HER2−), luminal B (ER+PR+/−HER2+), (ER−PR−HER2+), basal-like: ER−PR−HER2− cells were tested with CHCP at different power settings and at 4 different incubation time. The expression levels of the gene markers were determined at 4 different intervals after the treatment. The protein expression of BCL2A1 was only induced after CHCP treatment in TNBC cell lines (p < 0.01), whereas the HER2-positive and ER, PR positive cell lines showed little or no expression of BCL2A1. The BCL2A1 and TNF-alpha expression levels showed a significant correlation within TNBC cell lines (p < 0.01). Silencing BCL2A1 mRNA by siRNA increased the potency of the CHCP treatment. A Combination of CHCP and CPI203, a BET bromodomain inhibitor, and a BCL2A1 antagonist increased the CHCP-induced cell death (p < 0.05). Our results revealed that BCL2A1 is a key gene for resistance during CHCP induced cell death. This resistance in TNBCs could be reversed with a combination of siRNA or BCL2A1 antagonist-CHCP therapy.

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“…Over the past decade, low temperature plasma has been demonstrated to be effective as a therapeutic tool for the treatment of infections [ 8 , 9 ] and wound healing [ 10 , 11 , 12 ]. Since they are known to efficiently produce atomic and molecular species in both the ground electronic state (such as NO, the hydroxyl radical OH, and atomic oxygen) and vibrationally- or electronically-excited states (with internal energies ranging from 0.1 eV to beyond 10 eV), low temperature plasmas are capable of driving chemistry far from thermal equilibrium.…”

Section: Introductionmentioning

confidence: 99%

“…Since they are known to efficiently produce atomic and molecular species in both the ground electronic state (such as NO, the hydroxyl radical OH, and atomic oxygen) and vibrationally- or electronically-excited states (with internal energies ranging from 0.1 eV to beyond 10 eV), low temperature plasmas are capable of driving chemistry far from thermal equilibrium. Consequently, plasma has been applied successfully to wound healing [ 13 , 14 ], tissue regeneration [ 15 , 16 ], bacterial inactivation [ 8 , 17 ], and cancer treatment [ 18 , 19 ]. In addition, this occurs in the absence of the significant heating of tissue or organs.…”

Section: Introductionmentioning

confidence: 99%

Treatment of Fungal-Infected Diabetic Wounds with Low Temperature Plasma

et al. 2021

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Diabetes mellitus renders patients susceptible to chronic wounds and various infections. Regarding the latter, fungal infections are of particular concern since, although they are the source of significant morbidity and mortality in immunocompromised patients, they are generally resistant to conventional treatment and a definite treatment strategy has not yet been established. Herein, we report the treatment of skin wounds in a diabetic rat model, infected by Candida albicans, with low temperature helium plasma generated in a hand-held atmospheric jet device. A fungal infection was induced on two dorsal skin wounds of the diabetic rats, and one wound was treated with the plasma jet whereas the other served as a control. Histological analysis revealed accelerated skin wound healing and decreased evidence of fungal infection in the plasma-treated group, as compared to the control group. Regeneration of the epidermis and dermis, collagen deposition, and neovascularization were all observed as a result of plasma treatment, but without wound contraction, scar formation or any evidence of thermal damage to the tissue. These findings demonstrate that the He plasma jet is remarkably effective in diabetic skin wounds infected by Candida albicans, thereby providing a promising medical treatment option for diabetes mellitus patients with skin wound and fungal infections.

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Multi-Modal Biological Destruction by Cold Atmospheric Plasma: Capability and Mechanism

Cited by 26 publications

References 173 publications

Research Advances and Application Prospect of Low-Temperature Plasma in Tumor Immunotherapy

Research Advances and Application Prospect of Low-Temperature Plasma in Tumor Immunotherapy

BCL2A1 regulates Canady Helios Cold Plasma-induced cell death in triple-negative breast cancer

Treatment of Fungal-Infected Diabetic Wounds with Low Temperature Plasma

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