Cold-adapted tubulins in the glacier ice worm, Mesenchytraeus solifugus

Tartaglia, Lawrence J.; Shain, Daniel H.

doi:10.1016/j.gene.2008.07.025

Cited by 13 publications

(9 citation statements)

References 44 publications

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“…Their behaviors in winter seasons are unknown. Their physiology and phylogeny have also been investigated (9, 14, 18, 38); however, limited information is available on their ecological roles in glacial environments. In our previous study, the bacterial community structures physically associated with the ice worm were analyzed by cloning bacterial 16S rRNA genes (27).…”

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confidence: 99%

Bacterial Microbiota Associated with the Glacier Ice Worm Is Dominated by Both Worm-Specific and Glacier-Derived Facultative Lineages

et al. 2017

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The community structure of bacteria associated with the glacier ice worm Mesenchytraeus solifugus was analyzed by amplicon sequencing of 16S rRNA genes and their transcripts. Ice worms were collected from two distinct glaciers in Alaska, Harding Icefield and Byron Glacier, and glacier surfaces were also sampled for comparison. Marked differences were observed in bacterial community structures between the ice worm and glacier surface samples. Several bacterial phylotypes were detected almost exclusively in the ice worms, and these bacteria were phylogenetically affiliated with either animal-associated lineages or, interestingly, clades mostly consisting of glacier-indigenous species. The former included bacteria that belong to Mollicutes, Chlamydiae, Rickettsiales, and Lachnospiraceae, while the latter included Arcicella and Herminiimonas phylotypes. Among these bacteria enriched in ice worm samples, Mollicutes, Arcicella, and Herminiimonas phylotypes were abundantly and consistently detected in the ice worm samples; these phylotypes constituted the core microbiota associated with the ice worm. A fluorescence in situ hybridization analysis showed that Arcicella cells specifically colonized the epidermis of the ice worms. Other bacterial phylotypes detected in the ice worm samples were also abundantly recovered from the respective habitat glaciers; these bacteria may be food for ice worms to digest or temporary residents. Nevertheless, some were overrepresented in the ice worm RNA samples; they may also function as facultative gut bacteria. Our results indicate that the community structure of bacteria associated with ice worms is distinct from that in the associated glacier and includes worm-specific and facultative, glacier-indigenous lineages.

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confidence: 99%

Bacterial Microbiota Associated with the Glacier Ice Worm Is Dominated by Both Worm-Specific and Glacier-Derived Facultative Lineages

et al. 2017

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“…Also the microtubules associated with polysaccharides in neuronal cells are similarly stable (Tani and Ametani 1970). Another reason why the microtubules of O. umbellatum lipotubuloids are so stable may be the fact that this plant starts producing inflorescence early in a spring when the temperature oscillates around 0 o C. It is known that the cold treatment results in the appearance of stable microtubules due to cold adaptation (Detrich et al 2000, Tartaglia andShain 2008).…”

Section: Discussionmentioning

confidence: 99%

“Elaioplasts” of Haemanthus albiflos are true lipotubuloids: cytoplasmic domains rich in lipid bodies entwined by microtubules

et al. 2010

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In the cells of Haemanthus albiflos leaf epidermis there are structures containing lipids analogous to ''elaioplasts'' (Wakker in Jahrb F Wiss Bot 19:423-496, 1888). Ultrastructural analysis has shown that they are cytoplasmic domains-lipotubuloids, since they exhibit all the features of Ornithogalum umbellatum lipotubuloids. They are composed of numerous lipid bodies surrounded by microtubules, ER cisternae and vesicles, some mitochondria, Golgi structures, and microbodies. In the center of some lipotubuloids there are also autolytic vacuoles. Microtubules adjacent to H. albiflos lipid bodies were revealed only when taxol preincubation was used before fixing the epidermis in the mixture of glutaraldehyde and OsO 4 . The presence of tubulin in H. albiflos and O. umbellatum lipotubuloids was confirmed with use of the immunogold method involving antibodies against tubulin a. It is possible that the association of microtubules with lipid bodies may be more common than originally thought, but it is difficult to reveal due to the methodological problems.

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“…[1][2][3][4][5] Indeed, organisms have adapted to and perhaps even thrive in these environments, which are perceived as extreme from a human perspective; an adaptive mechanism organisms use to survive a stressful or changing environment is to reversibly enter into a hypometabolic state. 6 In a hypometabolic state, key biological processes such as development, cell division, metabolism and gene expression are either slowed or arrested.…”

Section: Hypometabolism and Arrestmentioning

confidence: 99%

Suspended animation, diapause and quiescence

Padilla

Ladage

2012

Cell Cycle

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Developing organisms require nutrients to support cell division vital for growth and development. An adaptation to stress, used by many organisms, is to reversibly enter an arrested state by reducing energy-requiring processes, such as development and cell division. This "wait it out" approach to survive stress until the environment is conductive for growth and development is used by many metazoans. Much is known about the molecular regulation of cell division, metazoan development and responses to environmental stress. However, how these biological processes intersect is less understood. Here, we review studies conducted in Caenorhabditis elegans that investigate how stresses such as oxygen deprivation (hypoxia and anoxia), exogenous chemicals or starvation affect cellular processes in the embryo, larvae or adult germline. Using C. elegans to identify how stress signals biological arrest can help in our understanding of evolutionary pressures as well as human health-related issues.

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Cold-adapted tubulins in the glacier ice worm, Mesenchytraeus solifugus

Cited by 13 publications

References 44 publications

Bacterial Microbiota Associated with the Glacier Ice Worm Is Dominated by Both Worm-Specific and Glacier-Derived Facultative Lineages

Bacterial Microbiota Associated with the Glacier Ice Worm Is Dominated by Both Worm-Specific and Glacier-Derived Facultative Lineages

“Elaioplasts” of Haemanthus albiflos are true lipotubuloids: cytoplasmic domains rich in lipid bodies entwined by microtubules

Suspended animation, diapause and quiescence

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