Cold active lipases – an update

Kavitha, M.

doi:10.1080/21553769.2016.1209134

Cited by 52 publications

(28 citation statements)

References 128 publications

(108 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Specific findings have relevance to bioremediation of pollutants from the petroleum industry, increasingly active in polar seas, and to biomanufacturing of cold-adapted enzymes. elevated amounts of unsaturated fatty acids in the membrane (keeping it from rigidifying in the cold), an overall higher content of polar amino acids (affecting protein thermolability), enzymes with high specific activity at low temperatures (10,19,25), and antifreeze molecules (18,22). However, to our knowledge, a comprehensive metabolic characterization of a psychrophilic extremophile is not available.…”

Section: Significancementioning

confidence: 99%

“…Able to grow at temperatures as low as −12°C (19), 34H has been used to study cold-adapted proteins (20) and enzymes (21), extracellular polysaccharide substances (18,22), and motility and chemohalotaxis at subzero temperatures (23,24). Recently, investigation of cold-adapted enzymes has increased in importance due to the potential economic and ecological advantages they can provide over their higher-temperature-requiring counterparts in industrial processes (25). Previous studies of cold-adapted microbes have revealed a variety of molecular adaptations that allow their activity and survival under extreme conditions, including…”

mentioning

confidence: 99%

See 1 more Smart Citation

Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H

Czajka

Abernathy

Benites

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Colwellia psychrerythraea 34H is a model psychrophilic bacterium found in the cold ocean—polar sediments, sea ice, and the deep sea. Although the genomes of such psychrophiles have been sequenced, their metabolic strategies at low temperature have not been quantified. We measured the metabolic fluxes and gene expression of 34H at 4 °C (the mean global-ocean temperature and a normal-growth temperature for 34H), making comparative analyses at room temperature (above its upper-growth temperature of 18 °C) and with mesophilic Escherichia coli. When grown at 4 °C, 34H utilized multiple carbon substrates without catabolite repression or overflow byproducts; its anaplerotic pathways increased flux network flexibility and enabled CO2 fixation. In glucose-only medium, the Entner–Doudoroff (ED) pathway was the primary glycolytic route; in lactate-only medium, gluconeogenesis and the glyoxylate shunt became active. In comparison, E. coli, cold stressed at 4 °C, had rapid glycolytic fluxes but no biomass synthesis. At their respective normal-growth temperatures, intracellular concentrations of TCA cycle metabolites (α-ketoglutarate, succinate, malate) were 4–17 times higher in 34H than in E. coli, while levels of energy molecules (ATP, NADH, NADPH) were 10- to 100-fold lower. Experiments with E. coli mutants supported the thermodynamic advantage of the ED pathway at cold temperature. Heat-stressed 34H at room temperature (2 hours) revealed significant down-regulation of genes associated with glycolytic enzymes and flagella, while 24 hours at room temperature caused irreversible cellular damage. We suggest that marine heterotrophic bacteria in general may rely upon simplified metabolic strategies to overcome thermodynamic constraints and thrive in the cold ocean.

show abstract

Section: Significancementioning

confidence: 99%

mentioning

confidence: 99%

Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H

Czajka

Abernathy

Benites

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Potential to produce laccase enzyme from the cold-adapted microorganisms of glacial sites and the hot springs of Himalaya have been reported through culture-dependent and culture-independent methods [91,92]. The cold-adapted microorganisms of Himalayan soils have also been reported as an abundant source of diverse cold-active lipases [93,94] that have importance in bioremediation, food industries, and cleaning industries [95]. The Himalayan soils are found to possess high diversity of bacteria that produce carbonic anhydrase, which is an important candidate for investigations related to carbon sequestration [96].…”

Section: Bioprospection Of Cold-tolerant Microbes: Versatile and Prommentioning

confidence: 99%

Microbial Ecology from the Himalayan Cryosphere Perspective

Dhakar

Pandey

2020

Microorganisms

View full text Add to dashboard Cite

Cold-adapted microorganisms represent a large fraction of biomass on Earth because of the dominance of low-temperature environments. Extreme cold environments are mainly dependent on microbial activities because this climate restricts higher plants and animals. Himalaya is one of the most important cold environments on Earth as it shares climatic similarities with the polar regions. It includes a wide range of ecosystems, from temperate to extreme cold, distributed along the higher altitudes. These regions are characterized as stressful environments because of the heavy exposure to harmful rays, scarcity of nutrition, and freezing conditions. The microorganisms that colonize these regions are recognized as cold-tolerant (psychrotolerants) or/and cold-loving (psychrophiles) microorganisms. These microorganisms possess several structural and functional adaptations in order to perform normal life processes under the stressful low-temperature environments. Their biological activities maintain the nutrient flux in the environment and contribute to the global biogeochemical cycles. Limited culture-dependent and culture-independent studies have revealed their diversity in community structure and functional potential. Apart from the ecological importance, these microorganisms have been recognized as source of cold-active enzymes and novel bioactive compounds of industrial and biotechnological importance. Being an important part of the cryosphere, Himalaya needs to be explored at different dimensions related to the life of the inhabiting extremophiles. The present review discusses the distinct facts associated with microbial ecology from the Himalayan cryosphere perspective.

show abstract

“…This rules out G. pullulans lipase as a coldactive enzyme, but it shows its greater thermostability compared to the cold-active lipase of Pichia lynferdii Y-7723 (5-30°C) (Bae et al, 2014) and with Candida rugosa lipase (35-40°C) (Vakhlu and Kour, 2006). Commonly, cold-active lipases exhibit high activity at low temperatures (0-20°C), but reduced thermostability (Bae et al, 2014;Kavitha, 2016). On the other hand, highly thermostable lipases show optimal temperature values of 50-75°C (Gonzalez et al, 2010;Sharma et al, 2011).…”

Section: Thermal Stability and Ph Effectmentioning

confidence: 99%

“…Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are hydrolases which constitute the third most important category of enzymes, after carbohydrases and proteases (Kavitha, 2016). They are ubiquitous enzymes, produced by plants, animals and microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Production, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulans

Demera¹,

Barahona²,

Barriga³

2019

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

The production of extracellular lipases from the Antarctic yeast Guehomyces pullulans is induced using an olive oil medium as an inductor substrate and a first characterization of its enzyme, using the protein extract obtained from the medium, is described. For this, the effect of pH and temperature on the lipase activity are evaluated and the enzyme kinetic for the lipase is determined. Lipase production was 0.27 U/mL, a high value compared to lipolytic activities in non-optimized media. However, this value can be increased by optimizing the culture medium. The lipase of G. pullulans has maximum activity at pH 8.0 and 40°C (thermal stability 40-50°C). Regarding the kinetic parameters, a K M = 3.7×10 −4 M was obtained, a value located in the range of industrial lipases. In addition, its kinetics presented the phenomenon of interfacial activation. The results presented in this work show the biotechnological potential of the lipase due its biochemical properties and are useful for later work directed to study other factors that affect the enzyme activity and potential biotechnological applications of the Guehomyces pullulans lipase.

show abstract

Cold active lipases – an update

Cited by 52 publications

References 128 publications

Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H

Model metabolic strategy for heterotrophic bacteria in the cold ocean based on Colwellia psychrerythraea 34H

Microbial Ecology from the Himalayan Cryosphere Perspective

Production, Extraction and Characterization of Lipases from the Antarctic Yeast Guehomyces pullulans

Contact Info

Product

Resources

About