Cold acclimation or grapeseed oil feeding affects phospholipid composition and mitochondrial function in duckling skeletal muscle

Chainier, François; Roussel, Damien; Georges, Béatrice; Meister, Roger; Rouanet, Jean‐Louis; Duchamp, Claude; Barré, Hervé

doi:10.1007/s11745-000-0625-8

Cited by 13 publications

(11 citation statements)

References 40 publications

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“…The cold-induced reduction in growth despite increased food intake is in agreement with previous studies [48] and indicates a high energy expenditure for regulatory thermogenesis. This is reinforced by a cold-induced increase in muscle mitochondria content (+38%, Table 1) that may help improve cellular ATP generation.…”

Section: Discussionsupporting

confidence: 92%

Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria

et al. 2010

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BackgroundAlthough identified in several bird species, the biological role of the avian homolog of mammalian uncoupling proteins (avUCP) remains extensively debated. In the present study, the functional properties of isolated mitochondria were examined in physiological or pharmacological situations that induce large changes in avUCP expression in duckling skeletal muscle.ResultsThe abundance of avUCP mRNA, as detected by RT-PCR in gastrocnemius muscle but not in the liver, was markedly increased by cold acclimation (CA) or pharmacological hyperthyroidism but was down-regulated by hypothyroidism. Activators of UCPs, such as superoxide with low doses of fatty acids, stimulated a GDP-sensitive proton conductance across the inner membrane of muscle mitochondria from CA or hyperthyroid ducklings. The stimulation was much weaker in controls and not observed in hypothyroid ducklings or in any liver mitochondrial preparations. The production of endogenous mitochondrial reactive oxygen species (ROS) was much lower in muscle mitochondria from CA and hyperthyroid ducklings than in the control or hypothyroid groups. The addition of GDP markedly increased the mitochondrial ROS production of CA or hyperthyroid birds up to, or above, the level of control or hypothyroid ducklings. Differences in ROS production among groups could not be attributed to changes in antioxidant enzyme activities (superoxide dismutase or glutathione peroxidase).ConclusionThis work provides the first functional in vitro evidence that avian UCP regulates mitochondrial ROS production in situations of enhanced metabolic activity.

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Section: Discussionsupporting

confidence: 92%

Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria

et al. 2010

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“…The yeast-based meal was higher in monounsaturated fatty acids than corn-based meal. The high 18:2(n-6) content of corn-based meal is typical of plant lipids and has been reported previously [30][31][32] .…”

Section: Effect Of Dietary Fatty Acid Composition On Mitochondrial Phsupporting

confidence: 61%

“…Dietary fatty acid manipulation is a wellestablished method known to alter membrane phospholipid fatty acyl profiles in several organisms. The effect of dietary fatty acids on cellular and sub-cellular membrane lipid fatty acid composition has been widely reported in cell cultures 16 , bacteria 35 , yeast 25 , insects 36 , birds 30 and mammals 32 . Dietary lipid modulates membrane lipid composition by two main mechanisms: (i) By providing the fatty acids available to be incorporated into membrane phospholipids, or (ii) By providing the precursors for other membrane lipid fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in <i>Drosophila melanogaster</i>

Ocloo¹

2010

Biokemistri

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Two groups of fruit flies (Drosophila melanogaster) were maintained on different diets.Mitochondria were isolated, proton leak was measured and phospholipid fatty acid composition determined. Mitochondria from flies fed on corn-base meal (containing high amounts of polyunsaturated fatty acids, 18:2(n-6) and 18:3(n-3) and low amounts of monounsaturated fatty acid, 18:1(n-9)) contained more polyunsaturated fatty acids in their membranes than mitochondria from flies fed on yeast-base meal (containing less amount of polyunsaturates and high amount of monounsaturates). The more polyunsaturated mitochondria were leakier to protons than the more monounsaturated mitochondria.

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“…Long-term cold exposure slowed the growth rate of ducklings (Fig.1) despite an increased food intake in the cold (Benistant et al, 1998;Chaînier et al, 2000). From day 17, body mass of cold-acclimated (CA) ducklings was significantly less (P<0.05) than that of TN ducklings, and the difference between CA and TN ducklings increased with increasing age.…”

Section: Body Mass and Growth Ratementioning

confidence: 97%

Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

Teulier

Rouanet

Letexier

et al. 2010

Journal of Experimental Biology

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) were obtained from a commercial stockbreeder (Eclosion Grimaud-lacorbière, Roussay, France). Birds were fed ad libitum with commercial mash (Moulin Guenard, 645000MI, Vonnas, France) and had free access to water. From the age of 10 days, ducklings were caged for Accepted 7 April 2010 SUMMARY Despite their lack of brown adipose tissue, some bird species develop regulatory non-shivering thermogenesis (NST) of skeletal muscle origin in response to cold acclimation. Mechanisms involved in avian NST are still unclear but may involve reduced energetic coupling in skeletal muscle mitochondria through the expression of an avian homologue of mammalian uncoupling proteins. The aim of this work was to investigate whether the expression of avian uncoupling protein (avUCP) would correlate with the capacity for cold-induced muscle NST. Various levels of cold acclimation were obtained by rearing 1-week-old ducklings (Cairina moschata) for 4 weeks at three different ambient temperatures (25°C, 11°C or 4°C). Muscle NST was measured by simultaneous recordings of metabolic rate and electromyographic activity (gastrocnemius muscle) at ambient temperatures (T a ) ranging from 27°C to -5°C. The expression of avUCP gene and mitochondrial bioenergetics were also determined in gastrocnemius muscle. Results showed that muscle NST capacity depends on the T a at which ducklings were acclimated, i.e. the lower the rearing temperature, the higher the capacity for NST. This increased metabolic heat production occurred in parallel with an upregulation of avUCP, which was not associated with a change in mitochondrial membrane conductance. The intensity of mitochondrial oxidative phosphorylation also increased in proportion with the harshness of cold, while the efficiency of ATP generation was equally effective in all three acclimation temperatures. In the absence of mitochondrial uncoupling, these data indicate a clear link between avUCP expression and the capacity of ducklings to adjust their muscular aerobic activity to cold exposure.

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Cold acclimation or grapeseed oil feeding affects phospholipid composition and mitochondrial function in duckling skeletal muscle

Cited by 13 publications

References 40 publications

Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria

Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria

Dietary fatty acids alter mitochondrial phospholipid fatty acyl composition and proton leak in <i>Drosophila melanogaster</i>

Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency

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