Cold acclimation of wheat (<i>Triticum aestivum</i>): Properties of enzymes involved in proline metabolism

Charest, Christiane; Ton Phan, Chon

doi:10.1111/j.1399-3054.1990.tb04391.x

Cited by 159 publications

(62 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4A and B). Apart from its pivotal role in regulating the amino acid metabolism with relation to osmotic adjustments, proline is also known as a possible free-radical scavenger with important implications in defence mechanisms against cold-induced oxidative stress [21,22]. A concomitant increase in proline and soluble sugars was detected after cold-exposure of wheat leaves with the maximum accumulation value reached after 21 days of treatment.…”

Section: Biochemical Parametersmentioning

confidence: 96%

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

et al. 2011

View full text Add to dashboard Cite

Cold represents one of the major abiotic factors influencing plant growth and development worldwide. We analysed the long-term responsiveness of an Iranian spring wheat (cv. Kohdasht) to cold from a proteomic point of view, in order to unravel the molecular mechanisms helping a cold-sensitive cultivar to survive exposure to suboptimal temperatures. Plants were grown at 20 or 4°C until entering the reproductive stage and a cross-comparison on the leaf proteomes was performed. Quantitative analyses on protein alterations occurring upon low-temperature exposure showed a reinforcement in ascorbate recycling (dehydroascorbate reductase, ascorbate peroxidase) and protein processing (proteasome subunit, cysteine proteinase), as well as the accumulation of the enzyme devoted to tetrapyrrole resynthesis (glutamate semialdehyde aminomutase). In contrast, among proteins down-regulated after cold stress, we could identify some key Krebs cycle enzymes (isocitrate dehydrogenase, malate dehydrogenase), together with many photosynthesis-related proteins (oxygen-evolving complex proteins, ATP synthase subunits, ferredoxin NADPH oxidoreductase and some Calvin cycle enzymes). Physiological and biochemical parameters (such as shoot apex dissection, chlorophyll, proline and sugar content determination) sustained proteomics findings allowing the present research to contribute to the current knowledge on these long-term responses, which may be crucial to stress adaptation under field conditions.

show abstract

Section: Biochemical Parametersmentioning

confidence: 96%

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The decrease in absorbance was recorded at 340 nm after starting the reaction by the addition of the enzyme extract. PDH activity was assayed by the reduction of NAD + at 340 nm (Charest and Phan 1990). The reaction mixture contained 0.15 M Na 2 CO 3 -HCl buffer (pH 10.3) containing 2.67 mM L-proline and 10 mM NAD + .…”

Section: Parametersmentioning

confidence: 99%

“…The homogenate was filtered and centrifuged at 12 000 g for 20 min (4 ºC) (Charest and Phan 1990). P5CS activity was measured as describe by Charest and Phan (1990). The reaction mixture (3 cm 3 ) contained: 100 mM Tris-HCl (pH 7.2), 25 mM MgCl 2 , 75 mM sodium glutamate, 5 mM ATP and the enzyme extract.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

See 1 more Smart Citation

Role of nitric oxide under saline stress: implications on proline metabolism

et al. 2008

View full text Add to dashboard Cite

The present work is focused on the possible relationship between nitric oxide and the induction of proline in response to salt stress. The plants were subjected to 100 mM NaCl and sodium nitroprusside (SNP; the donor of NO) at different concentrations. The plants showed lower NaCl-induced oxidative stress and proline accumulation after application of low concentrations of SNP together with the NaCl treatment. The reduction in the proline content was related to increased activity of proline dehydrogenase. These results suggest that the NO could be capable of mitigating damage associated with salt stress.Additional key words: ornithine-δ-aminotransferase, proline dehydrogenase, ∆ 1 -pyrroline-5-carboxylate synthetase, sodium nitroprusside.

show abstract

“…A positive correlation was found between leaf proline content and leaf Na content in the current investigation. Accumulation of proline in plant tissues as a response to salt stress has been attributed to enhanced activities of the enzyme involved in proline biosynthesis (Charest et al, 1990) and to inhibition of proline oxidase catabolizing enzyme (Yoshiba et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Evaluation for Salt Stress Tolerance in Two Strawberry Cultivars

Al-Shorafa¹

2014

American Journal of Agricultural and Biological Sciences

View full text Add to dashboard Cite

Salt stress conditions have been found to destroy the vital physiological process in plant where slower cell division and cell enlargement or both and limit mineral nutrient uptake with clearly nutritional imbalance. A greenhouse pot experiment was carried out at Agricultural Research Station, Mu'tah University, Jordan to evaluate growth responses and mineral composition of two strawberry cultivars to salt stress by different NaCl levels. Six NaCl levels (0, 25, 50, 75, 100 and 150 mM) and two strawberry cultivars, Camarosa and Albino, were used. Nutrient elements were added at the 0.5-strength Hoagland nutrient concentration. Distilled water supplemented with half strength Hoagland solution was used as control. Number of leaves and runners of both strawberry cultivars were significantly reduced by increasing NaCl level. Growth parameters (shoot, root and total dry weights and root: Shoot ratios) were significantly reduced with each increase in salinity level. Camarosa had higher value for all these parameters compared with Albino. Leaf chlorophyll content of both strawberry cultivars was significantly decreased when 25 mM NaCl or higher was applied. Regardless of NaCl level, Camarosa cultivar had significantly higher chlorophyll content compared with Albino. Elevated salinity level significantly increased leaf proline content of both cultivars. Albino leaves accumulated higher proline compared with Camarosa at salinized and non-salinized treatments. Both strawberry cultivars had significantly higher leaf and root Na and Cl contents under salinized conditions compared with non-salinized conditions. Each increase in NaCl level resulted in an increase in leaf and root Na and Cl contents of both cultivars. On contrast, leaf K content was decreased with salinity, root content of this mineral was significantly increased with salinity. It could be recommended to avoid growing 'Camarosa' and 'Albino' strawberry using irrigation water of 25 mM NaCl or more.

show abstract

Cold acclimation of wheat (Triticum aestivum): Properties of enzymes involved in proline metabolism

Cited by 159 publications

References 27 publications

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress

Role of nitric oxide under saline stress: implications on proline metabolism

Evaluation for Salt Stress Tolerance in Two Strawberry Cultivars

Contact Info

Product

Resources

About