Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of  Solanum commersonii ssp.

Tomé, Lívia Gracielle Oliveira; Silva, Adriano Bortolotti da; Pinto, César Augusto Brasil Pereira; Davide, Lisete Chamma; Pereira, Dalvana Sousa; Carvalho, Carlos Roberto Ribeiro

doi:10.1590/0103-8478cr20150477

Cited by 6 publications

(5 citation statements)

References 10 publications

(21 reference statements)

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“…Chromosome doubling can be induced by using colchicine, and it has been applied to various plants such as anise hyssop [ 31 ], banana [ 6 ], cassava [ 40 ], hot pepper [ 14 ], licorice [ 20 ], and potato [ 32 ]. The chromosome doubling that occurs in the plant cells is known as polyploidy.…”

Section: Introductionmentioning

confidence: 99%

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Tammu

Nuringtyas

Daryono

2021

Journal of Genetic Engineering and Biotechnology

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Background Productivity and quality of crops can be increased through polyploidy plants induced by colchicine. The use of colchicine has never been applied to Katokkon pepper, a local red pepper variety from North Toraja Indonesia. This pepper is characterized by its unique shape like the small-bell pod type of paprika and its strong spicy taste. Therefore, this study aimed to determine the effects of colchicine treatment on the ploidy level and morphological characters of Katokkon pepper. Results Flow cytometer analysis showed that all colchicine concentration treatments ranging from 0.025 to 0.1% with 24 h immersion time generated two plant groups based on their ploidy level; 50% of the total treatment plants was diploid while the rest plants were mixoploid. All colchicine treatment plants were significantly different in their width of stomata guard cells from the control plant. The effect of colchicine was also significantly on the plant height, thickness of fruit flesh, and the number of fruits per plant. Conclusions The results of this study showed that colchicine treatment had a significant effect on the ploidy level and several morphological characters of the Katokkon pepper. The colchicine treatment increased the number of fruits per plant and thicker flesh of fruits but reduced the size and weight of Katokkon pepper. Our findings provide essential information to obtain tetraploid Katokkon plants through colchicine treatment in further research. This study benefits as a preliminary step for increasing the productivity and quality of the local red peppers in Indonesia.

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Section: Introductionmentioning

confidence: 99%

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Tammu

Nuringtyas

Daryono

2021

Journal of Genetic Engineering and Biotechnology

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“…Ploidy manipulation methods were established to introgress genetic diversity from diploids to tetraploids ( Hermundstad and Peloquin, 1985a ; Peloquin et al., 1989a ; Jansky et al., 1990 ; Werner and Peloquin, 1991a ; Carputo et al., 2003 ). Breeders usually use di-haploids (2x 2EBN) derived from crossing tetraploid S. tuberosum (Group Tuberosum or Group Phureja) with haploid inducers to enable intercrossing with wild diploid species ( Hermundstad and Peloquin, 1985a ; Peloquin et al., 1989a ; Carputo et al., 2003 ), or anti-mitotic substances, such as colchicine and oryzalin, to increase the ploidy level of diploid species ( Tomé et al., 2016 ). Diploid F1 offspring that produces 2n gametes are valuable genetic material for capturing allelic diversity from most of the diploid wild potatoes ( Hermundstad and Peloquin, 1985a ; Peloquin et al., 1989a ; Jansky et al., 1990 ; Jansky and Peloquin, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

“…After many cycles of selection for adaptation and desired agronomic traits, superior diploid hybrids can be crossed with meiotic mutants producing 2n gametes to generate 4x progenies ( Peloquin et al., 1989a ; Peloquin et al., 1989b ; Werner and Peloquin, 1991a ; Masuelli et al., 1992 ; Carputo et al., 2003 ). Nodal segments of diploid S. malmeanum when treated with oryzalin (10-50µM for 24h) successfully induced tetraploid plants ( Tomé et al., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Solanum malmeanum, a promising wild relative for potato breeding

et al. 2023

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Crop wild relatives are gaining increasing attention. Their use in plant breeding is essential to broaden the genetic basis of crops as well as to meet industrial demands, for global food security and sustainable production. Solanum malmeanum (Solanum sect. Petota, Solanaceae) is a wild relative of potatoes (S. tuberosum) from Southern South America, occurring in Argentina, Brazil, Paraguay and Uruguay. This wild potato has been largely mistaken for or historically considered as conspecific with S. commersonii. Recently, it was reinstated at the species level. Retrieving information on its traits and applied uses is challenging, because the species name has not always been applied correctly and also because species circumscriptions and morphological criteria applied to recognize it have not been consistent. To overcome these difficulties, we performed a thorough literature reference survey, herbaria specimens’ identification revision and genebank database queries to review and update the information available on this potato wild relative, contributing to an increase in research on it to fully understand and explore its potential for potato breeding. Scarce studies have been carried out concerning its reproductive biology, resistance against pests and diseases as well as tolerance to abiotic stresses and evaluation of quality traits. The scattered information available makes it less represented in genebanks and genetic studies are missing. We compile, update and present available information for S. malmeanum on taxonomy, geographical distribution, ecology, reproductive biology, relationship with its closest relatives, biotic and abiotic stresses resistance and quality traits and discuss ways to overcome sexual barriers of hybridization and future perspectives for its use in potato breeding. As a final remark, we highlight that this species’ potential uses have been neglected and must be unlocked. Thus, further studies on morphological and genetic variability with molecular tools are fundamental for an efficient conservation and applied use of this promising genetic resource.

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“…Morphological and anatomical traits are associated with polyploid plants because they have larger cells and organs and are usually more robust than diploid ones (COSTA et al, 2011;JESUS et al, 2016;MANDAIL et al, 2022). Parameters such as leaf blade thickness, which can be indirectly measured by leaf disc mass, cell size, and stomata size and density (GANGA; CHEZHIYAN, 2002;FOSCHI et al, 2013;TOMÉ et al, 2016), can be used to considerably reduce the number of plants to be analyzed for ploidy determination, facilitating polyploidization research.…”

Section: Introductionmentioning

confidence: 99%

Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction

Borges,

Silveira,

Costa

et al. 2023

Rev. Caatinga

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This study aimed to evaluate the efficiency of the parameters fresh leaf mass and stomatal density in the estimation of ploidy and pre-selection of putative banana autotetraploids. Young plants of the diploid cultivar Ouro (AA), previously subjected to in vitro polyploidization with the antimitotic amiprofos-methyl - APM (0, 10, 20, 30, 40, and 60 µM L-1) and colchicine (2.5 mM L-1) were evaluated for survival, height, number of leaves, pseudostem diameter, leaf disc fresh mass, and stomatal density. Ploidy was determined by flow cytometry in a random sample of 200 plants to find the relationship between the genomic content and the analyzed variables. Spearman’s correlation analysis showed a strong correlation (0.84**) between leaf disc fresh mass and tetraploid plants, as well as an absence of significant correlation between stomatal density and ploidy levels. Thus, the pre-selection of plants was carried out using a reference value of leaf disc fresh mass and resulted in 688 plants pre-selected as putative tetraploids. Among them, 318 were confirmed as tetraploids, 291 as diploids, and 79 as mixoploids after flow cytometry analysis. The pre-selection efficiency was 46.2% of tetraploid plants from the total pre-selected. The results indicate that the use of leaf disc fresh mass is a simple, practical, and promising method to estimate ploidy when a high number of plants is obtained in in vitro banana polyploidization studies, reducing the total number of plants to have confirmed ploidy.

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Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp.

Abstract: Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp.Ciência Rural, v.46, n.11, nov, 2016. 1973Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp.

Cited by 6 publications

References 10 publications

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia

Solanum malmeanum, a promising wild relative for potato breeding

Ploidy estimation and pre-selection of banana autotetraploids after in vitro polyploidy induction

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