COL1A2 gene polymorphisms (<i>Pvu</i> II and <i>Rsa</i> I), serum calciotropic hormone levels, and dental fluorosis

Huang, Hui; Ba, Yue; Cui, Liuxin; Cheng, Xuemin; Zhu, Jingyuan; Zhang, Yuling; Yan, Pingping; Zhu, Cairong; Kilfoy, Briseis A.; Zhang, Yawei

doi:10.1111/j.1600-0528.2007.00424.x

Cited by 46 publications

(52 citation statements)

References 23 publications

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“…Rajasthan is severely affected by fluororsis problem. Huang et al, 2008 had reported that children with homozygous P allele of COL1A2 PvuII had about five times the risk of dental flouorosis compared to children with homozygous p allele after adjusting for age and gender [13]. COL1A2 gene polymorphism study is undertaken and study area is Jaipur District of Rajasthan, India.…”

Section: Resultsmentioning

confidence: 99%

“…This association between the COL1A2 gene and dental fluorosis was only observed for children who came from endemic fluoride villages. It is possible that this polymorphism may be in linkage disequilibrium with a causal mutation in the same gene [13]. A casecontrol study was conducted in two blocks of Jaipur district of Rajasthan, India to investigate the COL1A2 gene polymorphisms with relation to dental fluorosis in Indian children.…”

Section: Introductionmentioning

confidence: 99%

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Association of COL1A2 gene polymorphism with dental fluorosis in children of Jaipur district of Rajasthan, India

Meena¹,

Toteja²,

Bala³

et al. 2016

APJHS

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In India major districts are affected through dental fluorosis. In the Rajasthan, almost all the districts are affected from the fluorosis due to excess ingestion of fluoride through drinking water. Fluoride content calculated in the drinking water and blood through fluoride ion specific electrode method. A case-control study was conducted in two blocks (Jamwaramgarh, Amber) of Jaipur district of Rajasthan, India to investigate the COL1A2 gene polymorphisms with relation to dental fluorosis in Indian children (6-14 years). COL1A2 gene polymorphisms were genotyped using polymerase chain reaction-restriction fragment length polymorphism analysis. Two sites (rs2621208, rs414408) were analyzed for the polymorphism. PCR performed for respective amplicon size for RsaI (865bp) and PvuII (401bp).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association of COL1A2 gene polymorphism with dental fluorosis in children of Jaipur district of Rajasthan, India

Meena¹,

Toteja²,

Bala³

et al. 2016

APJHS

View full text Add to dashboard Cite

show abstract

“…However, the observed associations were not related to fluoride exposure (Wang et al, 2012). Huang et al (2008) found that homozygosity for the P allele of the COL1A2 PvuII (coding for the pro-alpha2 (I) chain of collagen) was associated with an increased risk for dental fluorosis compared to children carrying the homozygous genotype pp from the same fluoride-rich area (OR 4.85, 95 % CI 1.22-19.32), but the risk was not elevated when the control population was recruited from low-fluoride areas.…”

Section: Effects Of Genotypesmentioning

confidence: 99%

Scientific Opinion on Dietary Reference Values for fluoride

Products¹

2013

EFSA Journal

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Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for fluoride, which are provided as Adequate Intake (AI) from all sources, including non-dietary sources. Fluoride is not an essential nutrient. Therefore, no Average Requirement for the performance of essential physiological functions can be defined. Nevertheless, the Panel considered that the setting of an AI is appropriate because of the beneficial effects of dietary fluoride on prevention of dental caries. The AI is based on epidemiological studies (performed before the 1970s) showing an inverse relationship between the fluoride concentration of water and caries prevalence. As the basis for defining the AI, estimates of mean fluoride intakes of children via diet and drinking water with fluoride concentrations at which the caries preventive effect approached its maximum whilst the risk of dental fluorosis approached its minimum were chosen. Except for one confirmatory longitudinal study in US children, more recent studies were not taken into account as they did not provide information on total dietary fluoride intake, were potentially confounded by the use of fluoride-containing dental hygiene products, and did not permit a conclusion to be drawn on a dose-response relationship between fluoride intake and caries risk. The AI of fluoride from all sources (including non-dietary sources) is 0.05 mg/kg body weight per day for both children and adults, including pregnant and lactating women. For pregnant and lactating women, the AI is based on the body weight before pregnancy and lactation. Reliable and representative data on the total fluoride intake of the European population are not available. Fluoride has no known essential function in human growth and development and no signs of fluoride deficiency have been identified. Though fluoride is not essential for tooth development, exposure to fluoride leads to incorporation into the hydroxyapatite of the developing tooth enamel and dentin. The resulting fluorohydroxyapatite is more resistant to acids than hydroxyapatite. Thus, teeth which contain fluoroapatite are less likely to develop caries. Apart from incorporation of fluoride into the dentin and enamel of teeth before eruption, dietary fluoride exerts an anticaries effect on erupted teeth through contact with enamel during consumption, excretion into saliva and uptake into biofilms on teeth. In addition, fluoride interferes with the metabolism of oral microbial cells, by directly inhibiting, for example, glycolytic enzymes and cell membrane-associated H + ATPases in microbial cells after entry of hydrofluoric acid into their cytoplasm. © EuropeanIn bone, the partial substitution of fluoride for hydroxyl groups of apatite alters the mineral structure of the bone. Depending on the dose, fluoride can delay mineralisation. There is evidence from animal studies for a biphasic effect of fluoride on bone strength, with increases in both bone strength and bone ...

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“…Similarly, Dawson 5 and Huang et al, 4 concluded that some polymorphisms of the COL1A2 gene are involved in the development of dental fluorosis. However, Escobar-García et al, 6 and Pragya & Vandana 8 argue that there is no evidence of association between polymorphisms of the COL1A2 gene and dental fluorosis in populations exposed to high concentrations of fluoride.…”

Section: Referencesmentioning

confidence: 99%

“…Procollagen molecules organize themselves into long, thin fibrils that intertwine in the spaces around the cells, resulting in the formation of mature and strong Type I collagen fibers. 4 Due to its important role in bone formation and architecture, mutations in the COL1A2 gene have been associated with a broad spectrum of diseases of bone, cartilage and blood vessels. Likewise, the…”

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confidence: 99%

Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis

2018

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Objective: To compare the gene expression levels of collagen type I alpha 2 (COL1A2) in children with and without dental fluorosis. Methods: Cross-sectional study involving 92 children between 5 and 12 years of age. Socio-demographic characteristics, the presence of dental fluorosis by means of the Thylstrup-Fejerskov index, and gene expression analysis of COL1A2 in peripheral blood samples by reverse transcription polymerase chain reaction (RT-PCR) assays, were described. For the descriptive analysis, measures of central tendency, dispersion and proportions were used. Differences between the groups (p<0.05) were established by the Student t-test. Results: Mean age was 8.6 (SD=1.9) years, 51.1% were female; 54 children were diagnosed with fluorosis and 38 without fluorosis; prevalence of dental fluorosis was 58.7% (95% CI: 48.4% -68.9%). Gene expression of COL1A2 was statistically significantly lower (p<0.05) in the participants with dental fluorosis. Conclusion: There are differences in the expression levels of the COL1A2 gene among the population under study. Therefore, COL1A2 may be potentially involved in the development of dental fluorosis.Keywords: dental fluorosis; dental enamel; gene expression; collagen. Conflict of interests:The authors declare no conflicts of interest. Acknowledgements:The authors would like to thank Daniela Garrido, David Franco, Leydi Mendoza, Carlos Leal, Ana Anaya and the COO-SALUD Foundation for their great contribution and support in health day sessions, during which samples were collected. The authors would also like to thank the community of El Pozón for their participation and interest in this research.

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COL1A2 gene polymorphisms (Pvu II and Rsa I), serum calciotropic hormone levels, and dental fluorosis

Abstract: This study provides the first evidence of an association between polymorphisms in the COL1A2 gene with dental fluorosis in high fluoride exposed populations. Future studies are needed to confirm the association.

Cited by 46 publications

References 23 publications

Association of COL1A2 gene polymorphism with dental fluorosis in children of Jaipur district of Rajasthan, India

Association of COL1A2 gene polymorphism with dental fluorosis in children of Jaipur district of Rajasthan, India

Scientific Opinion on Dietary Reference Values for fluoride

Gene expression of collagen type I alpha 2 and its relationship with dental fluorosis

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