Coiled Bodies in the Meristematic Cells of the Root of Lupinus luteus L.

Wróbel, Bogdan; Smoliński, Dariusz Jan

doi:10.1023/a:1022850627155

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…The immunofluorescent and immunogold SmD localization experiments were performed using the mouse 7.13 antibody (1:10) (Testillano et al 1993 ). For Sm, we used a human αSm autoimmune serum against Sm that recognizes the plant Sm protein (1:200) (Pálfi et al 1989 ) according to a schedule established by Wróbel and Smoliński ( 2003 ). The mouse monoclonal antibody 4G3 (1:10) (Organon Teknika) recognizing the spliceosomal protein U2B″ was used (Habets et al 1989 ; Boudonck et al 1998 ).…”

Section: Methodsmentioning

confidence: 99%

Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

Smoliński

Wróbel

Noble

et al. 2011

Histochem Cell Biol

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Small nuclear ribonucleoproteins (snRNPs) play a fundamental role in pre-mRNA processing in the nucleus. The biogenesis of snRNPs involves a sequence of events that occurs in both the nucleus and cytoplasm. Despite the wealth of biochemical information about the cytoplasmic assembly of snRNPs, little is known about the spatial organization of snRNPs in the cytoplasm. In the cytoplasm of larch microsporocytes, a cyclic appearance of bodies containing small nuclear RNA (snRNA) and Sm proteins was observed during anther meiosis. We observed a correlation between the occurrence of cytoplasmic snRNP bodies, the levels of Sm proteins, and the dynamic formation of Cajal bodies. Larch microsporocytes were used for these studies. This model is characterized by natural fluctuations in the level of RNA metabolism, in which periods of high transcriptional activity are separated from periods of low transcriptional activity. In designing experiments, the authors considered the differences between the nuclear and cytoplasmic phases of snRNP maturation and generated a hypothesis about the direct participation of Sm proteins in a molecular switch triggering the formation of Cajal bodies.Electronic supplementary materialThe online version of this article (doi:10.1007/s00418-011-0861-8) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

Smoliński

Wróbel

Noble

et al. 2011

Histochem Cell Biol

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“…Grids were rinsed and contrasted according to Smoliński et al ( 2007 ). Immunogold labeling of Sm proteins and m3G snRNA was performed according to Wróbel and Smoliński ( 2003 ).…”

Section: Methodsmentioning

confidence: 99%

“…Despite the presence of splicing machinery elements, numerous transcription factors (Schul et al 1998 ), and even RNA polymerase II (Xie and Pombo 2006 ) in CBs, they are not considered to be a direct site of transcription and splicing. DNA (Moreno Díaz de la Espina et al 1982 ; Thiry 1994 ; Wróbel and Smoliński 2003 ), newly formed transcripts (Biggiogera and Fakan 1998 ; Kołowerzo et al 2009 ), and several crucial splicing factors, such as SC35 and U2AF (Gama-Carvalho et al 1997 ), were not observed in CBs. CBs are involved in tRNA maturation (Jarrous et al 2001 ), histone mRNA maturation (Wagner and Marzluff 2006 ), and telomere synthesis (Venteicher et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

“…Plant CBs are characterized as being similar to animal CBs. In plant cells, both free CBs and perinucleolar CBs were observed to accumulate splicing system elements, such as small nuclear RNAs (snRNAs) and Sm proteins (Chamberland and Lafontaine 1993 ; Beven et al 1995 ; Gulemetova et al 1998 ; Jennane et al 1999 ; Wróbel and Smoliński 2003 ; Kołowerzo et al 2009 ). The investigations of Boudonck et al ( 1999 ), using a U2B″–GFP fusion protein that illustrated the CB movement within the cell nucleus, demonstrated that nucleoplasmic CBs and nucleolar Cajal bodies presented different locations of the same structure.…”

Section: Introductionmentioning

confidence: 99%

“…The investigations of Boudonck et al ( 1999 ), using a U2B″–GFP fusion protein that illustrated the CB movement within the cell nucleus, demonstrated that nucleoplasmic CBs and nucleolar Cajal bodies presented different locations of the same structure. Plant CBs were shown to contain the rRNA processing machinery components, U3 snoRNA and fibrillarin (Olmedilla et al 1997 ; Boudonck et al 1999 ; Acevedo et al 2002 ; Wróbel and Smoliński 2003 ), but are devoid of rDNA (Lafontaine and Chamberland 1995 ) and rRNA (Wróbel and Smoliński 2003 ). Visualization of CBs in living plant cells revealed also that plant CBs exhibit dynamic movement, fusing together and splitting apart within the nucleus (Boudonck et al 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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mRNA accumulation in the Cajal bodies of the diplotene larch microsporocyte

Smoliński

Kolowerzo

2011

Chromosoma

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In microsporocytes of the European larch, we demonstrated the presence of several mRNAs in spherical nuclear bodies. In the nuclei of microsporocytes, we observed up to 12 bodies, ranging from 0.5 to 6 μm in diameter, during the prophase of the first meiotic division. Our previous studies revealed the presence of polyadenylated RNA (poly(A) RNA) in these bodies, but did not confirm the presence of nascent transcripts or splicing factors of the SR family. The lack of these molecules precludes the bodies from being the sites of synthesis and early maturation of primary transcripts (Kołowerzo et al., Protoplasma 236:13–19, 2009). However, the bodies serve as sites for the accumulation of splicing machinery, including the Sm proteins and small nuclear RNAs. Characteristic ultrastructures and the molecular composition of the nuclear bodies, which contain poly(A) RNA, are indicative of Cajal bodies (CBs). Here, we demonstrated the presence of several housekeeping gene transcripts—α-tubulin, pectin methylesterase, peroxidase and catalase, ATPase, and inositol-3-phosphate synthase—in CBs. Additionally, we observed transcripts of the RNA polymerase II subunits RPB2 and RPB10 RNA pol II and the core spliceosome proteins mRNA SmD1, SmD2, and SmE. The co-localization of nascent transcripts and mRNAs indicates that mRNA accumulation/storage, particularly in CBs, occurs in the nucleus of microsporocytes.Electronic supplementary materialThe online version of this article (doi:10.1007/s00412-011-0339-4) contains supplementary material, which is available to authorized users.

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Changes in poly(A) RNA and TMG snRNA distribution in the embryo sac of Hyacinthus orientalis L. before and after fertilization

et al. 2008

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The content and distribution of the poly(A) RNA and splicing machinery element--TMG snRNA in cells of the Hyacinthus orientalis L. mature embryo sac, during the progamic phase and after fertilization, were investigated. Using fluorescence in situ hybridization and immunofluorescence methods we showed that in the mature unfertilized embryo sac strong signal indicating poly(A) RNA and TMG snRNA appeared only in synergids and antipodal cells. In synergids accumulation of poly(A) RNA was shown in cytoplasm around the filiform apparatus. In the egg cell and central cell of the embryo sac accumulation of poly(A) RNA was very low and only low pool of TMG snRNA was observed in the nuclei of these cells. During the progamic phase, dramatic changes in the accumulation and distribution of poly(A) RNA and TMG snRNA were observed in the synergids. In these cells a considerable decrease in accumulation of TMG snRNA in the nucleus and poly(A) RNA in the region of the filiform apparatus occurred. In the egg cell and central cell the content of polyadenylated transcripts and TMG snRNA was still low. After fertilization a drastic increase in polyadenylated transcripts and TMG snRNA content was observed in the cells which undergo a fusion with the sperm cells (the zygote and the fertilized central cells). In contrast, a progressive decrease in poly(A) RNA and splicing snRNAs accumulation was observed in degenerating antipodal cells and synergids.

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Coiled Bodies in the Meristematic Cells of the Root of Lupinus luteus L.

Cited by 7 publications

References 28 publications

Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

mRNA accumulation in the Cajal bodies of the diplotene larch microsporocyte

Changes in poly(A) RNA and TMG snRNA distribution in the embryo sac of Hyacinthus orientalis L. before and after fertilization

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