COI-based species delimitation in Indochinese Tetraserica chafers reveal hybridisation despite strong divergence in male copulation organs

Dalstein, Vivian; Eberle, Jonas; Fabrizi, Silvia; Etzbauer, Claudia; Ahrens, Dirk

doi:10.1007/s13127-019-00398-z

Cited by 8 publications

(20 citation statements)

References 70 publications

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“…DNA-based species delimitation approaches may oversplit morphological entities (Ahrens et al, 2016), while at the same time the opposite may be also the case (Dalstein et al, 2019), even in the same taxon (as demonstrated here for the tribe Sericini). This particularly proved to be true in presence of incomplete lineage sorting and hybridization and if geographic bias is not excluded (match ratio <0.5; Dalstein et al, 2019). Extreme oversplitting has been reported for both mtDNA and nDNA, when sex-biased dispersal occurs, which also limits general dispersal (Eberle et al, 2019).…”

Section: Discussionmentioning

confidence: 71%

“…Sericini chafers proved to be a valuable model system, because of robust morphospecies assignments that were facilitated by highly dissimilar and morphologically complex male genitalia that serve as accurate species diagnostic trait (Dalstein et al, 2019;Eberle et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Specimens were presorted to morphospecies using the complex shape of their copulation organ, that is, aedeagus, which has been proven to be the best suited trait system to robustly infer species entities for this group (Dalstein et al, 2019;Eberle et al, 2016). For this purpose, male genitalia of all specimens were dissected.…”

Section: Sampling Was Conducted During 4 Weeks In April 2014 By Carolusmentioning

confidence: 99%

“…In order to infer in more detail the empirical behavior of species delimitation approaches that are currently in use in combination with particular genetic markers, it would be desirable to test commonly used methods on a dataset without geographic bias that still provides a sufficient number of closely related (i.e., congeneric) taxa that provide a sufficiently wide hypothesis space to test. Since low dispersal capacity might be a highly relevant factor for the outcome of such an investigation, we specifically investigate a soil-dwelling group of beetles (Sericini chafers) which have short emergence periods, little-specific host preferences, and a high degree of endemism (e.g., Ahrens, 2004Ahrens, , 2005Dalstein et al, 2019;.…”

Section: Introductionmentioning

confidence: 99%

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Excluding spatial sampling bias does not eliminate oversplitting in DNA‐based species delimitation analyses

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Sampling Was Conducted During 4 Weeks In April 2014 By Carolusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Excluding spatial sampling bias does not eliminate oversplitting in DNA‐based species delimitation analyses

LUKIC

Eberle

Thormann

et al. 2021

Ecology and Evolution

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“…Several molecular phylogenetic analyses confirmed the monophyly of Tetraserica (Ahrens & Vogler, 2008; Liu et al 2015; Eberle et al 2016). Based on a thorough examination of all type material of Asian mainland Sericini and an assessment of DNA-informed species boundaries (Dalstein et al 2019) we revise here the taxonomy of the Tetraserica species so far known from Indochina. We found 88 new species, which are described herein.…”

Section: Introductionmentioning

confidence: 99%

A monograph on the genus Tetraserica from the Indochinese region (Coleoptera, Scarabaeidae, Sericini)

Fabrizi¹,

Dalstein²,

Ahrens³

2019

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In this monograph on the Indochinese species of Tetraserica Ahrens, 2004 all species distributed in Thailand, Laos, Vietnam, Cambodia, Myanmar, and mainland Malaysia are covered as well as those of the Indian province Mizoram. From this revision, the following new combinations result: Tetrasericagressitti (Frey, 1972), comb. n., T.laotica (Frey, 1972), comb. n., T.satura (Brenske, 1898), comb. n., T.sejugata (Brenske, 1898), comb. n., T.siantarensis (Moser, 1922), comb. n., T.spinicrus (Frey, 1972), comb. n., T.vietnamensis (Frey, 1969), comb. n., and T.wapiensis (Frey, 1972), comb. n. Two new synonyms were found: Tetrasericamidoriae Kobayashi, 2017 (syn. n.) = T.laotica (Frey, 1972); T.graciliforceps Liu et al. 2014 (syn. n.) = T.satura (Brenske, 1898). The lectotypes of Tetrasericagestroi (Brenske, 1898), T.miniatula (Moser, 1915), and T.siantarensis (Moser, 1922) are designated. 116 Tetraserica species were recorded from Indochina, among which 88 new species are described: Tetrasericaallochangshouensissp. n., T.allomengeanasp. n., T.allosejugatasp. n., T.angkorthomensissp. n., T.angkorwatensissp. n., T.appendiculatasp. n., T.auriculatasp. n., T.bachmaensissp. n., T.banhuaipoensissp. n., T.bansanpakianasp. n., T.bolavensensissp. n., T.breviforcepssp. n., T.cattienensissp. n., T.champassakanasp. n., T.constantisp. n., T.cucphongensissp. n., T.curviforcepssp. n., T.desalvazzaisp. n., T.doiphukhaensissp. n., T.doipuiensissp. n., T.doisuthepensissp. n., T.dongnaiensissp. n., T.falciforcepssp. n., T.falciformissp. n., T.feresiantarensissp. n., T.filiforcepssp. n., T.fullerisp. n., T.phukradungensissp. n., T.geiseraesp. n., T.giulianaesp. n., T.infidasp. n., T.jaklisp. n., T.khaosoidaoensissp. n., T.kiriromensissp. n., T.koisp. n., T.kollaesp. n., T.konchurangensissp. n., T.kontumensissp. n., T.loeiensissp. n., T.lucaisp. n., T.microfurcatasp. n., T.microspinosasp. n., T.multiangulatasp. n., T.nahaeoensissp. n., T.nakaiensissp. n., T.namnaoensissp. n., T.neouncinatasp. n., T.nonglomensissp. n., T.nussisp. n., T.olegisp. n., T.pahinngamensissp. n., T.pailinensissp. n., T.parasetuliforcepssp. n., T.paratonkinensissp. n., T.petrpacholatkoisp. n., T.phatoensissp. n., T.phoupaneensissp. n., T.pluriuncinatasp. n., T.pseudoliangheensissp. n., T.pseudoruiliensissp. n., T.pseudouncinatasp. n., T.quadriforcepssp. n., T.quadrifurcatasp. n., T.rihaisp. n., T.romaesp. n., T.rubrithoraxsp. n., T.sapanasp. n., T.semidamadiensissp. n., T.semipingjiangensissp. n., T.semiruiliensissp. n., T.semishanensissp. n., T.setuliforcepssp. n., T.shanensissp. n., T.smetsisp. n., T.margheritaesp. n., T.sopponganasp. n., T.spanglerorumsp. n., T.spinotibialissp. n., T.subrotundatasp. n., T.tanahrataensissp. n., T.thainguyensissp. n., T.trilobiforcepssp. n., T.ululalatensissp. n., T.umphangensissp. n., T.varisp. n., T.veliformissp. n., T.vientianeensissp. n., and T.xiengkhouangensissp. n. A key to the Indochinese Tetraserica species is given and distributions as well as the habitus and male genitalia of all species are illustrated.

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Multiple species delimitation approaches with COI barcodes poorly fit each other and morphospecies – An integrative taxonomy case of Sri Lankan Sericini chafers (Coleoptera: Scarabaeidae)

Ranasinghe

Eberle

Thormann

et al. 2022

Ecology and Evolution

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DNA taxonomy including barcoding and metabarcoding is widely used to explore the diversity in biodiversity hotspots. In most of these hotspot areas, chafers are represented by a multitude of species, which are well defined by the complex shape of male genitalia. Here, we explore how well COI barcode data reflect morphological species entities and thus their usability for accelerated species inventorization. We conducted dedicated field surveys in Sri Lanka to collect the species-rich and highly endemic Sericini chafers (Coleoptera: Scarabaeidae). Congruence among results of a series of protocols for de novo species delimitation and with morphology-based species identifications was investigated. Different delimitation methods, such as the Poisson tree processes (PTP) model, Statistical Parsimony Analysis (TCS), Automatic Barcode Gap Discovery (ABGD), Assemble Species by Automatic Partitioning (ASAP), and Barcode Index Number (BIN) assignments, resulted in different numbers of molecular operational taxonomic units (MOTUs). All methods showed both over-splitting and lumping of morphologically identified species. Only 18 of the observed 45 morphospecies perfectly matched MOTUs from all methods. The congruence of delimitation between MOTUs and morphospecies expressed by the match ratio was low, ranging from 0.57 to 0.67. TCS and multirate PTP (mPTP) showed the highest match ratio, while (BIN) assignment resulted in the lowest match ratio and most splitting events. mPTP lumped more species than any other method. Principal coordinate analysis (PCoA) on a match ratio-based distance matrix revealed incongruent outcomes of multiple DNA delimitation methods, although applied to the same data. Our results confirm that COI barcode data alone are unlikely to correctly delimit all species, in particular, when using only a single delimitation approach. We encourage the integration of various approaches and data, particularly morphology, to validate species boundaries.

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COI-based species delimitation in Indochinese Tetraserica chafers reveal hybridisation despite strong divergence in male copulation organs

Cited by 8 publications

References 70 publications

Excluding spatial sampling bias does not eliminate oversplitting in DNA‐based species delimitation analyses

Excluding spatial sampling bias does not eliminate oversplitting in DNA‐based species delimitation analyses

A monograph on the genus Tetraserica from the Indochinese region (Coleoptera, Scarabaeidae, Sericini)

Multiple species delimitation approaches with COI barcodes poorly fit each other and morphospecies – An integrative taxonomy case of Sri Lankan Sericini chafers (Coleoptera: Scarabaeidae)

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