Coherent Anti-Stokes Raman Scattering Microscopy and Its Applications

Li, Shaowei; Li, Yanping; Yi, Rongxing; Liu, Liwei; Qu, Junle

doi:10.3389/fphy.2020.598420

Cited by 51 publications

(31 citation statements)

References 93 publications

(114 reference statements)

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“…In addition, several studies have shown that lipid metabolism in the brain of patients with AD is closely related to the formation of Aβ plaques [9,10]. Coherent anti-Stokes Raman scattering (CARS) microscopy is an emerging label-free vibrational imaging with chemical selection that has been widely applied in biology and medical research [11][12][13][14][15][16][17]. Because CARS can obtain spectral information while achieving unlabeled imaging, it is highly suitable for studying lipid metabolism and lipid distribution in the brain as well as for the identification of abnormal accumulation of Aβ in AD.…”

Section: Introductionmentioning

confidence: 99%

Distinguishing Amyloid β-Protein in a Mouse Model of Alzheimer’s Disease by Label-Free Vibrational Imaging

Luo

Zhang

et al. 2021

Biosensors

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Due to the increase in the average age of humans, Alzheimer’s disease (AD) has become one of the disorders with the highest incidence worldwide. Abnormal amyloid β protein (Aβ) accumulation is believed to be the most common cause of AD. Therefore, distinguishing the lesion areas can provide clues for AD diagnosis. Here, we present an optical spectroscopy and imaging approach based on coherent anti-Stokes Raman scattering (CARS). Label-free vibrational imaging of Aβ in a mouse model of AD was performed to distinguish the lesion areas by studying the spectra of regions with and without Aβ plaques. Raman spectra in Aβ and non-Aβ regions exhibited a specific difference in the intensity ratio of the wave peaks detected at 2850 and 2930 cm–1. In the non-Aβ region, the ratio of the peak intensity at 2850 cm–1 to that at 2930 cm–1 was approximately 1, whereas that in the Aβ region was 0.8. This label-free vibrational imaging may provide a new method for the clinical diagnosis and basic research of AD.

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Section: Introductionmentioning

confidence: 99%

Distinguishing Amyloid β-Protein in a Mouse Model of Alzheimer’s Disease by Label-Free Vibrational Imaging

Luo

Zhang

et al. 2021

Biosensors

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“…Bocklitz et al conducted a comparative study of hyperspectral SRS and CARS of human tissues, finding that cluster distributions in SRS and CARS are based on different spectral features, such that the combination of CARS and SRS data does not improve structure characterisation [134]. For further reading, Li et al published a recent comprehensive review of CARS that illustrated the range of applications in cell imaging, tissue imaging, biomedicine and material science [146].…”

Section: Coherent Anti-stokes Raman Scatteringmentioning

confidence: 99%

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Hilzenrat

Gill

McArthur

2022

Journal of Biophotonics

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The past decade has seen an increasing demand for more complex, reproducible and physiologically relevant tissue cultures that can mimic the structural and biological features of living tissues. Monitoring the viability, development and responses of such tissues in real‐time are challenging due to the complexities of cell culture physical characteristics and the environments in which these cultures need to be maintained in. Significant developments in optics, such as optical manipulation, improved detection and data analysis, have made optical imaging a preferred choice for many three‐dimensional (3D) cell culture monitoring applications. The aim of this review is to discuss the challenges associated with imaging and monitoring 3D tissues and cell culture, and highlight topical label‐free imaging tools that enable bioengineers and biophysicists to non‐invasively characterise engineered living tissues.

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“…In addition, the backscattered CARS signal is generally much weaker than forward-directed ones. As a result, NIVI is gradually becoming a successful microscopic technique that has been used in studies of animal tumor models and skin diseases [ 63 , 64 , 65 , 66 ]. In the future, the development of supercontinuum sources and epi-detection techniques may eventually lead to broader applicability of NIVI-based OCT to address in vivo biological studies and medical applications.…”

Section: Nonlinear Interferometric Vibrational Imaging and Its Applic...mentioning

confidence: 99%

Molecular Contrast Optical Coherence Tomography and Its Applications in Medicine

Wang

Gao

et al. 2022

IJMS

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The growing need to understand the molecular mechanisms of diseases has prompted the revolution in molecular imaging techniques along with nanomedicine development. Conventional optical coherence tomography (OCT) is a low-cost in vivo imaging modality that provides unique high spatial and temporal resolution anatomic images but little molecular information. However, given the widespread adoption of OCT in research and clinical practice, its robust molecular imaging extensions are strongly desired to combine with anatomical images. A range of relevant approaches has been reported already. In this article, we review the recent advances of molecular contrast OCT imaging techniques, the corresponding contrast agents, especially the nanoparticle-based ones, and their applications. We also summarize the properties, design criteria, merit, and demerit of those contrast agents. In the end, the prospects and challenges for further research and development in this field are outlined.

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Coherent Anti-Stokes Raman Scattering Microscopy and Its Applications

Cited by 51 publications

References 93 publications

Distinguishing Amyloid β-Protein in a Mouse Model of Alzheimer’s Disease by Label-Free Vibrational Imaging

Distinguishing Amyloid β-Protein in a Mouse Model of Alzheimer’s Disease by Label-Free Vibrational Imaging

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Molecular Contrast Optical Coherence Tomography and Its Applications in Medicine

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