Coherent anti-Stokes Raman scattering microscopy for quantitative characterization of mixing and flow in microfluidics

Schafer, Dawn; Müller, Markus; Bonn, Mischa; Marr, David W. M.; Maarseveen, Jan van; Squier, Jeff

doi:10.1364/ol.34.000211

Cited by 11 publications

(10 citation statements)

References 19 publications

(21 reference statements)

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“…Examples of such studies include the characterization of atherosclerotic plaques, 7 dynamic mapping of lipid phase transitions, 19 and the study of chemical reactions in microfluidic devices. 20,21 However, in many biomedical imaging studies of tissue specimens, rapid scanning is the preferred mode of operation as large areas (millimeters) need to be examined with high spatial resolution (sub-micrometer). For these particular studies, a loss of spectral information is compensated by a much higher information density in the spatial domain, and picosecond CARS microscopy is a sensible choice.…”

Section: Introductionmentioning

confidence: 99%

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

et al. 2011

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Abstract. The lipid distribution in the mouse meibomian gland was examined with picosecond spectral antiStokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH 2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Introductionmentioning

confidence: 99%

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

et al. 2011

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“…We have shown that it is possible to quantify the local concentrations of miscible fluids within a microfluidic device using CARS microscopy. In the case of nonreacting species, this information reports on the mass transport within the device and can potentially be used to determine unknown diffusion coefficients or fluid flow behavior in shear-sensitive media . In the case of reacting species, the local concentration images that are determined by quantitative CARS microscopy can be used to investigate fast reaction kinetics.…”

Section: Phase Extractionmentioning

confidence: 99%

“…In the case of nonreacting species, this information reports on the mass transport within the device and can potentially be used to determine unknown diffusion coefficients or fluid flow behavior in shear-sensitive media. 64 In the case of reacting species, the local concentration images that are determined by quantitative CARS microscopy can be used to investigate fast reaction kinetics. As an example, we studied the rapid proton transfer between acetic acid and pyridine.…”

Section: Feature Articlementioning

confidence: 99%

Quantitative Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

et al. 2011

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The ability to observe samples qualitatively at the microscopic scale has greatly enhanced our understanding of the physical and biological world throughout the 400 year history of microscopic imaging, but there are relatively few techniques that can truly claim the ability to quantify the local concentration and composition of a sample. We review coherent anti-Stokes Raman scattering (CARS) as a quantitative, chemically specific, and label-free microscopy. We discuss the complicating influence of the nonresonant response on the CARS signal and the various experimental and mathematical approaches that can be adopted to extract quantitative information from CARS. We also review the uses to which CARS has been employed as a quantitative microscopy to solve challenges in material and biological science.

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“…Examples for CNM are imaging techniques involving second harmonic generation (SHG) [6], third harmonic generation (THG) [7] or coherent anti-Stokes Raman scattering (CARS) [8]. While CARS microscopy exploits resonances in the vibronic level structure of the sample [9], SHG and THG microscopy typically apply off-resonant frequency conversion processes. In the case of strongly focussed laser beams (which provide large signals and large resolution) SHG and THG appear only at interfaces [7,10], while in the bulk medium the harmonics interfere destructivly due to the Gouy phase shift [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of laser polarization and interface orientation in harmonic generation microscopy

Petzold¹,

Büchel²,

Halfmann³

2012

Opt. Express

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We present systematic experimental investigations on the effects of laser polarization and interface orientation in second and third harmonic generation microscopy. We find that the laser polarization has no measurable effect on signal strength and resolution in third harmonic microscopy, while the second harmonic strongly depends upon the polarization direction of the driving laser. Moreover, we observe a strong effect of the interface orientation with respect to the laser beam direction-both in second and third harmonic generation. This affects the signal strength, as well as the obtained transversal and longitudinal resolution in microscopic imaging. As an (on the first glance) surprising feature, also surfaces parallel to the optical axis of the laser beam yield strong harmonic signal. This enables applications of harmonic microscopy in specific geometries. As an example we monitor the flow of immiscible microfluids in lateral cut by third harmonic microscopy.

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Coherent anti-Stokes Raman scattering microscopy for quantitative characterization of mixing and flow in microfluidics

Cited by 11 publications

References 19 publications

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

Quantitative Coherent Anti-Stokes Raman Scattering (CARS) Microscopy

Effects of laser polarization and interface orientation in harmonic generation microscopy

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