Coffee attenuates induction of insulin resistance in high sucrose-diet fed rats

Abstract

Several epidemiological evidence indicate that consumption of coffee is associated with a lower risk of type 2 diabetes mellitus; however, there is dearth of experimental data to support these observations. Given that associations do not necessarily infer causality; the present study investigate the impact of coffee (300 mg/kg BW) by oral gavage on normal and high sucrose-diet (HSD) fed (30 % w/v) rats. HSD rats were insulin resistant and had significantly elevated levels of insulin, MDA, triglyceride, LDL while HDL level, SOD and GSH activities were significantly reduced. In contrast, coffee consumption significantly up-regulate glucose tolerance, insulin sensitivity, HDL level, SOD and GSH activities while MDA, triglyceride, LDL levels were reduced. These findings suggest that coffee attenuates glucose intolerance, insulin insensitivity, lipid disorders, peroxidation and oxidative stress in sucrose-induced insulin resistant rat.Coffee is probably the most frequently ingested beverage worldwide. The numerous beneficial health effects of coffee consumption have received considerable scientific attention (Ranheim and Halvorsen, 2005). Epidemiological evidence indicates that consumption of coffee is consistently associated with a lower risk of T2DM (Bidel et al 2006). Since associations do not necessarily infer causality; experimental study using a representative diabetic animal model is importantly meaningful. A small number of these studies have been performed (Yamacuchi et al, 2010; Matsuda et al, 2011). No report of its effects on diet-induced diabetic model and diabeticinduced oxidative stress. In the present study, we employed sucrose-induced diabetic rats to investigate the effects of coffee on glucose regulation, T2DM, and its antioxidant potential under diabetic condition.Male Sprague-Dawley rats (120-150g) were used.• Group 1: normal diet (ND).• Group 2: ND supplemented with coffee, 300mg/kg BW (ND+COF).• Group 3: high sucrose-diet (HSD).• Group 4: HSD supplemented with coffee, 300mg/kg BW (HSD+COF).