Coexpression network analysis of the genes regulated by two types of resistance responses to powdery mildew in wheat

Zhang, Juncheng; Zheng, H.; Li, Yiwen; Li, Hongjie; Liu, Xin; Qin, Huanju; Dong, Lingli; Wang, Daowen

doi:10.1038/srep23805

Cited by 31 publications

(29 citation statements)

References 88 publications

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“…Triticum urartu has been shown to be a good source for powdery mildew resistance genes, and contains more nucleotide‐binding and leucine‐rich repeat domain (NB‐LRR) genes than rice, maize or sorghum (Ling et al ., ). Recently, three candidate NB‐LRR genes associated with Bgt resistance were identified based on expression analyses (Zhang et al ., ). However, the expression of Pm60 was not induced by Bgt E09 infection; therefore, this gene could not be identified by genomic strategies, such as RNA‐seq analysis.…”

Section: Discussionmentioning

confidence: 97%

The NB‐LRR gene Pm60 confers powdery mildew resistance in wheat

et al. 2017

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Powdery mildew is one of the most devastating diseases of wheat. To date, few powdery mildew resistance genes have been cloned from wheat due to the size and complexity of the wheat genome. Triticum urartu is the progenitor of the A genome of wheat and is an important source for powdery mildew resistance genes. Using molecular markers designed from scaffolds of the sequenced T. urartu accession and standard map-based cloning, a powdery mildew resistance locus was mapped to a 356-kb region, which contains two nucleotide-binding and leucine-rich repeat domain (NB-LRR) protein-encoding genes. Virus-induced gene silencing, single-cell transient expression, and stable transformation assays demonstrated that one of these two genes, designated Pm60, confers resistance to powdery mildew. Overexpression of full-length Pm60 and two allelic variants in Nicotiana benthamiana leaves induced hypersensitive cell death response, but expression of the coiled-coil domain alone was insufficient to induce hypersensitive response. Yeast two-hybrid, bimolecular fluorescence complementation and luciferase complementation imaging assays showed that Pm60 protein interacts with its neighboring NB-containing protein, suggesting that they might be functionally related. The identification and cloning of this novel wheat powdery mildew resistance gene will facilitate breeding for disease resistance in wheat.

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Section: Discussionmentioning

confidence: 97%

The NB‐LRR gene Pm60 confers powdery mildew resistance in wheat

et al. 2017

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“…Exploring the gene pools of wheat wild relatives for new and durable Bgt-resistance could lead to the development of wheat cultivars with robust powdery mildew resistance. However, mapping, characterization and finally cloning R-genes derived from hexaploid wheat, which has three closely-related and independent genomes (A, B and D), is challenging due a huge genome size estimated at ~17 Gb [32,43,44]. This effort is further complicated if the genes are originating from wheat wild relatives as a result of low wheat-alien homoeologous recombination that is strictly controlled by Ph genes in hexaploid wheat genetic backgrounds.…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptome sequencing or RNA-seq can provide extensive data pre and post pathogen infection for the analysis and has been widely used to discover R genes and reveal the transcriptional expression pattern of genes involved in pathogen-defense responsive pathways in crops including hexaploid wheat [28,32,[43][44][45][46]. For example, Stpk-V, a putative serine/threonine-protein kinase gene derived from D. villosum against powdery mildew was successfully cloned and characterized based on integrating microarray analysis of transcriptomes before and after Bgt-inoculation with physical mapping of chromosomal segment locating Pm21 [45].…”

Section: Introductionmentioning

confidence: 99%

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

Zhang

Tian

et al. 2019

Preprint

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Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% of 2Ss#1 segment on the long arm of chromosome 2Ss#1. In this study, we performed RNA-Seq on three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines with Bgt-isolates inoculation at 0, 12, 24, and 48 hours after inoculation. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homoelogous (Ph) genes. Analysis of the 46 ph1b-induced 2Ss#1L recombinants with different Bgt-responses using 28 2Ss#1L-specific molecular markers in the interval FL0.72-0.87 where Pm57 is located, and the flanking intervals, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese spring (T. aestivum L.) with other model species we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for further cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

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“…tauschii Coss. (2n = 2x = 14, DD), together with advances in RNA sequencing (RNA-seq) and bulked segregant RNA-seq (BSR-seq) have greatly enhanced the pace of conventional map-based cloning of R-genes from wheat relatives [28][29][30][31][32][33][34][35]. For example, up till now at least five cataloged Pm genes including Pm2, Pm3b, Pm8, Pm21, Pm60 have been cloned.…”

Section: Introductionmentioning

confidence: 99%

“…Transcriptome sequencing or RNA-seq can provide extensive data pre and post-pathogen infection for the analysis and has been widely used to discover R-genes and reveal the transcriptional expression pattern of genes involved in pathogen-defense responsive pathways in crops including hexaploid wheat [28,32,[42][43][44][45]. For example, Stpk-V, a putative serine/threonine-protein kinase gene derived from D. villosum against powdery mildew was successfully cloned and characterized based on integrating microarray analysis of transcriptomes before and after Bgt-inoculation with physical mapping of chromosomal segment locating Pm21 [44].…”

Section: Introductionmentioning

confidence: 99%

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

Zhang

Tian

et al. 2020

IJMS

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Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75–0.87, which represents 12% segment of the long arm of chromosome 2Ss#1. In this study, we performed RNA-seq using RNA extracted from leaf samples of three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines at 0, 12, 24, and 48 h after inoculation with Bgt isolates. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homologous (Ph) genes. After analyzing the 46 ph1b-induced 2Ss#1L recombinants in the region where Pm57 is located with different Bgt-responses, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese Spring (T. aestivum L.) with other model species, we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

show abstract

Coexpression network analysis of the genes regulated by two types of resistance responses to powdery mildew in wheat

Cited by 31 publications

References 88 publications

The NB‐LRR gene Pm60 confers powdery mildew resistance in wheat

The NB‐LRR gene Pm60 confers powdery mildew resistance in wheat

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

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