Coenzyme Q10 ameliorates inflammation, oxidative stress, and testicular histopathology in rats exposed to heat stress

Abstract

Objectives: Our work was aimed at investigating the impact and regulatory mechanism of coenzyme Q10 ( CoQ10) on exogenous scrotal heat stress (HS)-induced testicular injuries in rats. Methods: The rats ( n = 32) were assigned into four groups: control, HS control, HS+ CoQ10, and CoQ10. To induce HS, rats’ testicles were immersed in a water bath at 43°C for 20 min, every other day for 8 weeks. Moreover, treatment with CoQ10 (10 mg/kg) immediately started before inducing HS and continued for 8 weeks. Key findings: HS decreased the activity of the testicular antioxidant system, superoxide dismutase, glutathione peroxidase, and catalase, while the amount of lipid peroxidation (malondialdehyde) was increased. The index of apoptosis and mRNA expression of caspase 3 and Bax were increased, while the mRNA expression levels of Bcl-2, 3β-HSD, and 17β-HSD3 decreased after HS. Exposure to HS decreased the serum testosterone level but increased the activation of pro-inflammatory cytokines (interleukin 1 beta and tumor necrosis factor-alpha). Deleterious effects of HS on the mentioned parameters were reduced when the rats were treated with CoQ10. Conclusions: CoQ10 could suppress the degenerative effects following testicular hyperthermia via its antiapoptotic, anti-inflammation, antioxidative, and androgen synthesis effects.

The effect of Fumaria parviflora on the expression of sexual hormones along with their receptors in testicles of adult rats induced by varicocele

“…The right testicles of animals were harvested for the assessment of the expression rate of SF‐1, CatSper‐1, 3β‐HSD, CatSper‐2, LHCGR, and 17β‐HSD3 genes. The RNA contents of spermatozoa were extracted by the TRIzol reagent (Invitrogen, Paisley, UK) based on the protocol established by our previous research (Sigaroodi et al, 2019; Delkhosh et al, 2021). The DNase I enzyme (Invitrogen Life Technologies, Carlsbad, CA, USA) was utilized to eliminate possible DNA contamination in extracted RNA samples, according to the manufacturer's instructions.…”

Coenzyme Q10 Supplementation enhances testicular volume and hemodynamics, reproductive hormones, sperm quality, and seminal antioxidant capacity in goat bucks under summer hot humid conditions

“…CoQ10 was introduced into animal feeds for the treatment of various OS-induced organ dysfunctions, including cattle (Wafa and El-Nagar 2021 ), rabbits (Elokil et al 2019 ), and birds (Sharideh et al 2020 ), horses (Nemec Svete et al 2021 ), rats (Delkhosh et al 2021 ) and humans (Nadjarzadeh et al 2014 ). CoQ10 supplementation enhances testicular function and fertility in aged breeding roosters and heat-stressed rabbits by lowering OS, increasing total antioxidant capacity and testosterone levels, and upregulating testicular melatonin receptors (Elokil et al 2019 ; Sharideh et al 2020 ).…”

The combination of HT-ac and HBET improves the cognitive and learning abilities of heat-stressed mice by maintaining mitochondrial function through the PKA–CREB–BDNF pathway

“…Likewise, similar effects in differences of these factors were observed using in vitro cultured mouse neural stem cells. 12–16 These findings identified that these responses were related to the accumulation of oxidative damage and inflammation in cells following heat stress. Similarly, the expression of PKA, CREB and BDNF also changed after heat stress.…”