Coded aperture structured illumination digital holographic microscopy for superresolution imaging

Lai, Xin Ji; Tu, Han Yen; Lin, Yu Chih; Cheng, Chau Jern

doi:10.1364/ol.43.001143

Cited by 25 publications

(14 citation statements)

References 25 publications

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“…2(f) . Here an enlarged spatial frequency is obtained and its spatial resolution can achieve less than half of the wavelength, such spatial resolution can be referred in different names as, resolution enhancement, super-resolved, sub-diffraction limit or superresolution 11 , 18 , 19 , 37 . And Fig.…”

Section: Methodsmentioning

confidence: 99%

Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Vinoth

Lai

Lin

et al. 2018

Sci Rep

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Digital holographic microtomography is a promising technique for three-dimensional (3D) measurement of the refractive index (RI) profiles of biological specimens. Measurement of the RI distribution of a free-floating single living cell with an isotropic superresolution had not previously been accomplished. To the best of our knowledge, this is the first study focusing on the development of an integrated dual-tomographic (IDT) imaging system for RI measurement of an unlabelled free-floating single living cell with an isotropic superresolution by combining the spatial frequencies of full-angle specimen rotation with those of beam rotation. A novel ‘UFO’ (unidentified flying object) like shaped coherent transfer function is obtained. The IDT imaging system does not require any complex image-processing algorithm for 3D reconstruction. The working principle was successfully demonstrated and a 3D RI profile of a single living cell, Candida rugosa, was obtained with an isotropic superresolution. This technology is expected to set a benchmark for free-floating single live sample measurements without labeling or any special sample preparations for the experiments.

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Section: Methodsmentioning

confidence: 99%

Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Vinoth

Lai

Lin

et al. 2018

Sci Rep

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“…The illumination pattern is carefully controlled by Φ 1 and Φ 2 . Accordingly, the SI wavefront spectrum ( O SI ( u , v )) of the two overlapped passbands can be expressed as 18 .…”

Section: Working Principlementioning

confidence: 99%

“…The symbols A i and A d are the transfer functions corresponding to the illumination and detection 4F systems of the object illumination 18 as shown in Fig. 1(a), and are the recorded aberrations, and the cut-off frequency, , θ x , y is the diffracted angle along x and y axes.…”

Section: Working Principlementioning

confidence: 99%

“…From the recorded holograms, as explained in the second step, the overlapped passbands are separated and synthesized to obtain an enlarged spatial frequency coverage. Thus, the resulting spatial frequency coverage () of the SI-HT system can be expressed as 18 where, the symbol m , n denotes the number of passbands, and the symbol M , N denotes the number of hologram acquisitions. The calibrated measurement procedures of AWC SI-HT system showing each steps with its corresponding experimental results are described in Fig.…”

Section: Working Principlementioning

confidence: 99%

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Adaptive wavefront correction structured illumination holographic tomography

Vinoth

Lai

et al. 2019

Sci Rep

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In this study, a novel adaptive wavefront correction (AWC) technique is implemented on a compactly developed structured illumination holographic tomography (SI-HT) system. We propose a mechanical movement-free compact scanning architecture for SI-HT systems with AWC, implemented by designing and displaying a series of computer-generated holograms (CGH) composed of blazed grating with phase Fresnel lens on a phase-only spatial light modulator (SLM). In the proposed SI-HT, the aberrations of the optical system are sensed by digital holography and are used to design the CGH-based AWC to compensate the phase aberrations of the tomographic imaging system. The proposed method was validated using a standard Siemens star target, its potential application was demonstrated using a live candida rugosa sample, and its label-free three-dimensional refractive index profile was generated at its subcellular level. The experimental results obtained reveal the ability of the proposed method to enhance the imaging performance in both lateral and axial directions.

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“…Because the slit is replaced with Hadamard coding template, it has the advantage of multichannel detection, which could better solve the contradiction between spectral resolution and light flux. 2–6…”

Section: Introductionmentioning

confidence: 99%

Restoration Method of Hadamard Coding Spectral Imager

Tang

et al. 2020

Appl Spectrosc

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Hadamard coding spectral imaging technology is a computational spectral imaging technology, which modulates the target’s spectral information and recovers the original spectrum by inverse transformation. Because it has the advantage of multichannel detection, it is being studied by more researchers. For the engineering realization of push-broom coding spectral imaging instrument, it will inevitably be subjected to push-broom error, template error and detection noise, the redundant sampling problem caused by detector. Therefore, three restoration methods are presented in this paper: firstly, the one is the least squares solution, the two is the zero-filling inverse solution by extending the coding matrix in the redundant coding state to a complete higher order Hadamard matrix, the three is sparse method. Secondly, the numerical and principle analysis shows that the inverse solution of zero-compensation has better robustness and is more suitable for engineering application; its conditional number, error expectation and covariance are better and more stable because it directly uses Hadamard matrix, which has good generalized orthogonality. Then, a real-time spectral reconstruction method is presented, which is based on inverse solution of zero-compensation. Finally, simulation analysis shows that spectral data could be destructed relative accuracy in the error condition; however, the effect of template noise and push error on reconstruction is much greater than that of detection error. Therefore, in addition to reducing the detection noise as much as possible, lower template noise and more accurate push controlling should be guaranteed specifically in engineering realization.

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Coded aperture structured illumination digital holographic microscopy for superresolution imaging

Cited by 25 publications

References 25 publications

Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Adaptive wavefront correction structured illumination holographic tomography

Restoration Method of Hadamard Coding Spectral Imager

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