Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Vinoth, B. R.; Lai, Xin Ji; Lin, Yu Chih; Tu, Han Yen; Cheng, Chau Jern

doi:10.1038/s41598-018-24408-w

Cited by 49 publications

(9 citation statements)

References 35 publications

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“…For label-free RI tomographic image analysis, live candida rugosa (ATCC 14830) is used as a sample and is loaded into the microchannel (µ-Slide I 0.4 Luer from ibidi) for the AWC SI-HT measurement procedures. The tomographic images are reconstructed 45,46 , and the different slices correspond to xy and xz directions, as elucidated in Fig. 6.…”

Section: Experiments and Resultsmentioning

confidence: 99%

Adaptive wavefront correction structured illumination holographic tomography

Vinoth

Lai

et al. 2019

Sci Rep

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In this study, a novel adaptive wavefront correction (AWC) technique is implemented on a compactly developed structured illumination holographic tomography (SI-HT) system. We propose a mechanical movement-free compact scanning architecture for SI-HT systems with AWC, implemented by designing and displaying a series of computer-generated holograms (CGH) composed of blazed grating with phase Fresnel lens on a phase-only spatial light modulator (SLM). In the proposed SI-HT, the aberrations of the optical system are sensed by digital holography and are used to design the CGH-based AWC to compensate the phase aberrations of the tomographic imaging system. The proposed method was validated using a standard Siemens star target, its potential application was demonstrated using a live candida rugosa sample, and its label-free three-dimensional refractive index profile was generated at its subcellular level. The experimental results obtained reveal the ability of the proposed method to enhance the imaging performance in both lateral and axial directions.

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Section: Experiments and Resultsmentioning

confidence: 99%

Adaptive wavefront correction structured illumination holographic tomography

Vinoth

Lai

et al. 2019

Sci Rep

Self Cite

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“…A maximal handling velocity is also defined according to the number of traps, in order to assist the user and help the trapped objects’ retention. The velocity control mode can be enabled independently for translations and rotations, and is suitable for long displacements like sample chamber exploration or for continuous rotation of an object like micro-pump [27] or cell rotation for tomographic imaging [28].…”

Section: Methodsmentioning

confidence: 99%

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

et al. 2019

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Single-cell manipulation is considered a key technology in biomedical research. However, the lack of intuitive and effective systems makes this technology less accessible. We propose a new tele–robotic solution for dexterous cell manipulation through optical tweezers. A slave-device consists of a combination of robot-assisted stages and a high-speed multi-trap technique. It allows for the manipulation of more than 15 optical traps in a large workspace with nanometric resolution. A master-device (6+1 degree of freedom (DoF)) is employed to control the 3D position of optical traps in different arrangements for specific purposes. Precision and efficiency studies are carried out with trajectory control tasks. Three state-of-the-art experiments were performed to verify the efficiency of the proposed platform. First, the reliable 3D rotation of a cell is demonstrated. Secondly, a six-DoF teleoperated optical-robot is used to transport a cluster of cells. Finally, a single-cell is dexterously manipulated through an optical-robot with a fork end-effector. Results illustrate the capability to perform complex tasks in efficient and intuitive ways, opening possibilities for new biomedical applications.

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“… 13 Combination of both approaches can also be envisaged. 14 , 15 , 16 Using the angular variety of the acquisitions allows, under adequate assumptions, 17 to retrieve a 3D quantitative information about the complex refractive index map (refraction and absorption contributions) of the investigated sample, with improved resolution compared to conventional intensity‐only transmission microscopy. 18 , 19 , 20 Commercial microscopes have been made available on the market.…”

Section: Introductionmentioning

confidence: 99%

3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy

Verrier

Taddese

Abbessi

et al. 2022

Journal of Microscopy

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Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high-resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification.

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Integrated dual-tomography for refractive index analysis of free-floating single living cell with isotropic superresolution

Cited by 49 publications

References 35 publications

Adaptive wavefront correction structured illumination holographic tomography

Adaptive wavefront correction structured illumination holographic tomography

Tele–Robotic Platform for Dexterous Optical Single-Cell Manipulation

3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy

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