Fertility and Sterility

2008

DOI: 10.1016/j.fertnstert.2007.10.054

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Coculture with cumulus cells improves maturation of mouse oocytes denuded of the cumulus oophorus: observations of nuclear and cytoplasmic events

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Effects Of Heat Stress On Oocyte Maturation1

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Supporting the Growth Capacitation And Maturation Of The O1

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Cited by 47 publications

(39 citation statements)

References 47 publications

(62 reference statements)

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“…Nishi et al (2003) found that the rate of mitochondrial congregation was significantly higher in the in vivo than in vitro matured mouse oocytes. Miki et al (2006) and Ge et al (2008) reported that mouse oocytes matured under optimized conditions essentially resembled in vivo matured oocytes in mitochondrial distribution. According to Sun & Schatten (2006), while peripheral cortical granule migration in oocytes is controlled by microfilaments, mitochondria movement is mediated by microtubules.…”

Section: Discussionmentioning

confidence: 97%

“…The barrelshaped spindle has been considered less normal than the tine-pole spindle and it occurred more frequently in the in vitro matured than in vivo matured oocytes (Sanfins et al 2003). Ge et al (2008) found that while the percentage of oocytes with tine-pole spindles was lower, that of oocytes with even-pole spindles was higher significantly following maturation with cumulus denuded than with cumulus intact. Roth & Hansen (2005) noticed that a subset of bovine oocytes possessed misshapen metaphase-I spindles with disorganized microtubules and unaligned chromosomes after heatshock during in vitro maturation.…”

Section: Effects Of Heat Stress On Oocyte Maturationmentioning

confidence: 99%

See 1 more Smart Citation

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Wang¹,

Sui²,

et al. 2009

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The objectives of this study were to investigate the effect of heat stress during in vitro maturation on the developmental potential of mouse oocytes and to determine whether the deleterious effect was on the nuclear or cytoplasmic component. While rates of oocyte nuclear maturation (development to the metaphase II stage) did not differ from 37 to 40 8C, rates for blastocyst formation decreased significantly as maturation temperature increased from 38.5 to 39 8C. Chromosome spindle exchange showed that while blastocyst formation did not differ when spindles matured in vivo or in vitro at 37, 40 or 40.7 8C were transplanted into in vivo matured cytoplasts, no blastocyst formation was observed when in vivo spindles were transferred into the 40 8C cytoplasts. While oocytes reconstructed between 37 8C ooplasts and 37 or 40 8C karyoplasts developed into 4-cell embryos at a similar rate, no oocytes reconstituted between 40 8C ooplasts and 37 8C spindles developed to the 4-cell stage. Immunofluorescence microscopy revealed impaired migration of cortical granules and mitochondria in oocytes matured at 40 8C compared with oocytes matured at 37 8C. A decreased glutathione/GSSG ratio was also observed in oocytes matured at 40 8C. While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 8C, spindle assembling was affected and MAD2 was activated in some of the oocytes matured at 40.7 8C. It is concluded that 1) oocyte cytoplasmic maturation is more susceptible to heat stress than nuclear maturation, and 2) cytoplasmic rather than nuclear components determine the pre-implantation developmental capacity of an oocyte.

“…Nishi et al (2003) found that the rate of mitochondrial congregation was significantly higher in the in vivo than in vitro matured mouse oocytes. Miki et al (2006) and Ge et al (2008) reported that mouse oocytes matured under optimized conditions essentially resembled in vivo matured oocytes in mitochondrial distribution. According to Sun & Schatten (2006), while peripheral cortical granule migration in oocytes is controlled by microfilaments, mitochondria movement is mediated by microtubules.…”

Section: Discussionmentioning

confidence: 97%

“…The barrelshaped spindle has been considered less normal than the tine-pole spindle and it occurred more frequently in the in vitro matured than in vivo matured oocytes (Sanfins et al 2003). Ge et al (2008) found that while the percentage of oocytes with tine-pole spindles was lower, that of oocytes with even-pole spindles was higher significantly following maturation with cumulus denuded than with cumulus intact. Roth & Hansen (2005) noticed that a subset of bovine oocytes possessed misshapen metaphase-I spindles with disorganized microtubules and unaligned chromosomes after heatshock during in vitro maturation.…”

Section: Effects Of Heat Stress On Oocyte Maturationmentioning

confidence: 99%

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Wang¹,

Sui²,

et al. 2009

Self Cite

View full text Add to dashboard Cite

The objectives of this study were to investigate the effect of heat stress during in vitro maturation on the developmental potential of mouse oocytes and to determine whether the deleterious effect was on the nuclear or cytoplasmic component. While rates of oocyte nuclear maturation (development to the metaphase II stage) did not differ from 37 to 40 8C, rates for blastocyst formation decreased significantly as maturation temperature increased from 38.5 to 39 8C. Chromosome spindle exchange showed that while blastocyst formation did not differ when spindles matured in vivo or in vitro at 37, 40 or 40.7 8C were transplanted into in vivo matured cytoplasts, no blastocyst formation was observed when in vivo spindles were transferred into the 40 8C cytoplasts. While oocytes reconstructed between 37 8C ooplasts and 37 or 40 8C karyoplasts developed into 4-cell embryos at a similar rate, no oocytes reconstituted between 40 8C ooplasts and 37 8C spindles developed to the 4-cell stage. Immunofluorescence microscopy revealed impaired migration of cortical granules and mitochondria in oocytes matured at 40 8C compared with oocytes matured at 37 8C. A decreased glutathione/GSSG ratio was also observed in oocytes matured at 40 8C. While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 8C, spindle assembling was affected and MAD2 was activated in some of the oocytes matured at 40.7 8C. It is concluded that 1) oocyte cytoplasmic maturation is more susceptible to heat stress than nuclear maturation, and 2) cytoplasmic rather than nuclear components determine the pre-implantation developmental capacity of an oocyte.

“…The intracellular content of GSH, the level of glutathione, was measured as described by Ge et al[9]. After postmortem carcass preservation, GV oocytes were collected and washed three times in phosphate buffered solution (PBS).…”

Section: Methodsmentioning

confidence: 99%

Effects of Postmortem Interval on Mouse Ovary Oocyte Survival and Maturation

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et al. 2014

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To study the time- and temperature-dependent survival of ovarian oocytes collected from postmortem carcass, ICR mice were killed and placed for different periods (0, 1, 2, 4, 6, 8 and 10 h) at different temperatures (25°C, 4°C and 37°C). After preservation, oocyte morphology, germinal vesicle (GV) oocyte number, oocyte meiotic maturation percentage, mitochondrial distribution and intracellular glutathione (GSH) level were evaluated. The results showed no surviving oocytes could be collected by 2h, 6h, and 12 h after carcass preservation at 37°C, 25°C and 4°C, respectively. The number of collected GV oocytes in the ovary deceased as the preservation time lasted at the same temperature. Meanwhile at the same point in time, the ratio of germinal vesicle breakdown (GVBD) and the first polar body emission (PBE) gradually reduced as preservation temperature increased. In addition, the percentage of abnormal mitochondrial distribution in the preserved oocytes was obviously higher than that in the control oocytes, while GSH level was not altered in collected oocytes. Unexpectedly, neither chromosome arrangement nor spindle organization was affected as long as the oocytes from preserved carcasses could complete maturation. These data are helpful for proper use of ovary oocytes from postmortem carcass of valuable individuals.

“…This is believed to contribute to the protein stores of the oocyte in anticipation of embryonic development. Experiments comparing the development of denuded oocytes with that of intact COCs have reported that spindle formation, mitochondrial congregation, and cortical granule distribution are impaired in denuded oocytes (Liu et al, 2005;Ge et al, 2008b). Furthermore, this defect can be partially corrected if denuded oocytes are matured with a cumulus cell monolayer in co-culture (Ge et al, 2008b).…”

Section: Supporting the Growth Capacitation And Maturation Of The Omentioning

confidence: 99%

The antral follicle: a microenvironment for oocyte differentiation

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2012

Int. J. Dev. Biol.

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Mammalian reproduction hinges upon the timely ovulation of a fully differentiated oocyte. This event is the culmination of a complex and dynamic developmental relationship between the oocyte and the antral follicle housing it; the antral follicle constitutes a specialized microenvironment or niche, uniquely suited to the needs of the oocyte as it approaches ovulation. During this time, the oocyte must complete its final growth, capacitation, and nuclear and cytoplasmic maturation. Its microenvironment-the antral follicle-is in turn responsible for the integrity of these processes and the production of a high quality oocyte. Components of the antral follicle, including three distinct somatic cell types (theca, granulosa and cumulus), the basal lamina, and follicular fluid, each have active and regulatory roles in oocyte differentiation. Several milestones in antral folliculogenesis also have an influence on oocyte development. This review will discuss the antral follicle microenvironment with specific attention to its importance in oocyte differentiation. As assisted reproductive technologies (ART) often require stages of oocyte differentiation to occur in vitro rather than in vivo, current knowledge of the antral follicle microenvironment will also be discussed with respect to its clinical applications.

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